FastQCFastQC Report
Sat 30 Sept 2023
SRR18220723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18220723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125008745
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC18066911.4452516901917543Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA9808210.7846019092504288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA9573260.7658072241266001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG8325050.665957409619623No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG7639150.6110892481961963No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC6948580.5558475129079969Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC6943460.5554379415616083No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA6606030.5284454299577201No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG6557990.5246024988091833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT6017890.4813975214294008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5933580.47465319326259936No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA5522170.4417426956810102No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC4941050.39525634786590325No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4934150.3947043864811218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC4714070.377099218138699No Hit
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC4711010.376854435263709TruSeq Adapter, Index 6 (100% over 35bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG4655210.37239074754330187No Hit
AAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC3890370.3112078279003601TruSeq Adapter, Index 6 (100% over 36bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC3857380.3085688125258757No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3811710.3049154681138508No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3365730.26923956400010257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3282060.2625464322515997No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC3067580.24538923256928946No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3065330.24520924516120854No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCT2948200.2358395006685332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAACT2884390.23073505777535802Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2843790.22748728498954213No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT2839660.22715690810270914No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC2777620.22219405530389094No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA2760630.22083495038687095No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2641600.21131321652737176No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC2630370.21041487937503894No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA2562380.20497605987485115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2553290.20424891074620422No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2413180.19304089485899567Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2397100.19175458484924393Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC2311910.18493986160728196No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2307270.18456868757461728No Hit
TAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAAC2257240.1805665675629333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2247600.17979542151231098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2232700.17860350489879728TruSeq Adapter, Index 6 (100% over 35bp)
ACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCTG2219290.17753077994663494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2215690.17724280009370544Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2212870.17701721587557734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA2198200.17584369797488966No Hit
GAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCT2078540.16627156764112783TruSeq Adapter, Index 6 (100% over 50bp)
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC2003640.16027998681212263No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA1940420.15522274061706642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1937400.15498115751822Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA1891450.15130541467318948No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC1885130.15079985004249102Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1847940.14782485817292224No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1789740.1431691838838955No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT1769970.14158769452489103No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1768280.14145250398282136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA1746880.13974062374596274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT1742670.1394038473068424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTC1735920.13886388508259961TruSeq Adapter, Index 6 (98% over 50bp)
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC1702890.13622166993197155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTG1690430.13522493966322116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1680850.13445859327681434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTATTAGAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1642450.1313868081788998TruSeq Adapter, Index 6 (100% over 35bp)
TAGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTG1636480.13090924158945838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA1630750.13045087365687896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCT1621030.12967332805396936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1620550.12963493074024543No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACG1584520.12675273237884277No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT1551440.12410651750803514No Hit
ACCTCTAGCATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1537470.12298899568986153TruSeq Adapter, Index 6 (100% over 35bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA1530270.12241303598400255No Hit
CTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC1519650.12156349541786056Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1519090.1215186985518493TruSeq Adapter, Index 6 (100% over 49bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1438650.11508394872694708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1435110.11480076853823307No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1428730.11429040424331914No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1417010.11335286983322646No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA1402910.11222494874258597No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1389760.11117302233535742No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC1384410.11074505227614276No Hit
ATTGATCATCGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1374200.1099283094154733TruSeq Adapter, Index 6 (100% over 36bp)
ATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1363970.10910996666673199Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1361050.10887638300824475Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA1325220.1060101835275604No Hit
CGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1320070.10559821234906407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1296280.1036951454876217No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1279130.10232324146602703No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCAC1277660.1022056496927475No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1262410.10098573503797674No Hit
ACCTCTAGCATTACAAGTATTAGAGGCAAGATCGGAAGAGCACACGTCTG1262110.10096173671689929Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1256980.10055136542647476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT1250240.10001220314626788Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)