FastQCFastQC Report
Sat 30 Sept 2023
SRR18220724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18220724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144239259
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC16026131.1110796125207494No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA15434751.0700796792085572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG15296111.0604678716492852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG12301910.8528822239720464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC11448580.7937214929813249No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT11076490.767924771438267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA9539780.6613858159102162No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC9211780.638645821107553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT9020630.6253935345022814No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC8774810.6083510176657244No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG8417940.5836094873449121No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG8325310.5771875186907331No Hit
ACTGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC8317690.576659229787086TruSeq Adapter, Index 12 (100% over 46bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG8097980.5614268997319238No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC8068250.5593657410566704TruSeq Adapter, Index 12 (100% over 50bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGA7770140.5386979976096521Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA7745890.5370167632378089Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT7539100.522680167124264Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA7422990.5146303476226262No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA6898750.47828518031973527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGACTGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG6742260.4674358456042817TruSeq Adapter, Index 12 (97% over 45bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC6063100.4203501905122793No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG5834550.4045049898654845TruSeq Adapter, Index 12 (100% over 50bp)
CTGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT5734440.39756443840300093TruSeq Adapter, Index 12 (97% over 48bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA5621750.38975172494473226No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT5372730.3724873545003445No Hit
TCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACT5266710.3651370671558983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT5107800.3541199556495226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA4528760.31397554531252825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAA4490450.3113195416512782Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4351490.301685548731223No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT4174030.28938237959195284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4167350.2889192601856059TruSeq Adapter, Index 12 (100% over 49bp)
TCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAAC3982760.2761217734763876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT3978900.2758541625619416TruSeq Adapter, Index 12 (97% over 46bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA3885210.26935870490016867Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTG3815950.2645569608756795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT3744140.25957842725745006TruSeq Adapter, Index 12 (100% over 50bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3571300.2475955592644857No Hit
GAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT3488490.2418544038693377TruSeq Adapter, Index 12 (100% over 50bp)
TGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT3392970.2352320736755865TruSeq Adapter, Index 12 (100% over 48bp)
AAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT3335830.23127060019075668TruSeq Adapter, Index 12 (100% over 50bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACG3322220.230327029064951No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3268310.22658948906552548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAA3190290.22118042078959932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA3106610.2153789489448223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT3043590.21100981945560326No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC3039730.2107422085411573No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA2967170.20571167798359252Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2942160.20397775337988944No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2935700.2035298864090809No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA2931900.20326643524978175No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACAC2857160.19808476692188218No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2842600.19707533300625177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2748900.19057918205195437Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2735450.1896467036065403TruSeq Adapter, Index 12 (100% over 50bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTG2678470.18569632280210205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT2668580.18501065649539977No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2642960.18323444104770395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2641700.18314708618962056No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA2551540.17689636078898605No Hit
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC2544210.17638817736854845Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT2533990.17567963240853865TruSeq Adapter, Index 12 (100% over 50bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2526290.17514579716469564No Hit
CCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACG2513500.17425907602589666No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC2496290.17306591959128131TruSeq Adapter, Index 12 (100% over 50bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC2422040.16791822259708086No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCT2387250.16550625790444473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAA2323670.1610983040338553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG2300610.15949957147242416No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG2169210.15038970770086943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC2062490.14299088987971023TruSeq Adapter, Index 12 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2013550.13959791626494697No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1999550.13862730673068696No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC1986990.13775653131995083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC1948180.13506586303247717No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1918990.133042142153545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1882070.13048250615319648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGTTCAGATCGGAAGAGCACACGTCT1879490.13030363668188283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT1837990.12742647270532637Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCACAC1832620.12705417461968518No Hit
TCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT1828140.126743579568722Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT1815740.12588389683837742No Hit
TTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAAC1756470.12177475204583517Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCTG1753270.12155289843800432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCAC1717280.1190577386424316No Hit
TCAGCTCACATAGTGACGCAGGCAGTGCGACCAGATCGGAAGAGCACACG1708980.11848230584712031No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT1703470.11810030166613654No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1674000.1160571685965192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTC1661140.11516559441004894No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1652320.11455411040346512No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1603850.11119372153735203No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1572820.10904243483391718No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCT1533750.10633374094080725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT1486270.10304198803461684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAA1477260.10241733147006808Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1454600.1008463306096158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)