FastQCFastQC Report
Fri 29 Sept 2023
SRR18220725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18220725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87973866
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC25161982.860165313185168Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA10080021.1457970938778568TruSeq Adapter, Index 2 (100% over 36bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG9600131.0912479394732977TruSeq Adapter, Index 2 (100% over 35bp)
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA5292870.6016411737549422Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC5101310.5798665253610658Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCT4557210.5180186124820296TruSeq Adapter, Index 2 (100% over 50bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC4313050.49026491571940234No Hit
ACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA4049060.46025714045578037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3759210.42730985586105763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG3735380.42460109687574715No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3718590.42269257554283224No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG3574650.40633089831473357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTC3335440.379139868651447TruSeq Adapter, Index 2 (98% over 50bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA3298140.3748999731352036No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC3039730.34552647714720186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG2981600.3389188330088847TruSeq Adapter, Index 2 (100% over 35bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2853020.3243031288405582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTATTAGAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG2822840.3208725645863966TruSeq Adapter, Index 2 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2796890.31792282494439883TruSeq Adapter, Index 2 (100% over 49bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG2508120.28509830408044134No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC2352800.2674430608744647No Hit
ATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2321890.2639295174319155Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCTG2295270.26090361880879487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2254120.2562260933263976No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG2212950.25154629444157883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2180470.24785428890893574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC2154820.24493865030326165No Hit
GTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2133640.2425311171388103Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2124640.2415080860491001No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2016340.22919761193625388No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1909030.21699967124327582No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG1831460.2081822799511846No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1631340.1854346153208727No Hit
GTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCTG1597320.1815675578017681Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1570000.1784620900938922No Hit
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC1508020.17141681598942124Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA1504130.17097463921842426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1501590.1706859171108838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA1495960.17004595432920955No Hit
GTGACTGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1478810.1680965117527062TruSeq Adapter, Index 2 (100% over 43bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT1448850.164690954925182No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA1444160.1641578420573219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1417420.16111830301967178No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1384230.1573455917010627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1304260.14825539211838207Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1288730.14649009513802658No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1277430.14520562276983484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1243080.1413010541107742No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1242520.14123739884297No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGAGATCGGAAGAGCACACG1242000.141178290380009No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC1235650.1404564851111579No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC1164460.13236430919155015No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1150030.13072404934438142No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTG1093180.12426190296104526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAATGCCTAAAGGAAAGATCCAAAAAGATAAGATCGGAAGAGCACACG1092610.1241971109920303No Hit
CGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1083850.12320136073137904Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1079490.12270575900347497TruSeq Adapter, Index 2 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA1062960.12082679190204054No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG1055920.12002655424964499Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAATGCCTAAAGGAAAGATCCAAAAAGATAAGATCGGAAGAGCACACGT1045370.11882733447226247No Hit
CTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC1043670.11863409526642833Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1028890.116954050876882TruSeq Adapter, Index 2 (100% over 35bp)
AAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1027730.11682219353643046TruSeq Adapter, Index 2 (100% over 37bp)
AACATCACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACAC1015880.11547520260164536No Hit
ACCTCTAGCATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1012110.11504666624517784TruSeq Adapter, Index 2 (100% over 35bp)
TGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAA1003740.11409524733174735Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAGATCGGAAGAGCACACGTCTG988960.11241520294220105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG984110.1118639028549683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAAGATCGGAAGAGCACACGTCTG974860.11081245423498838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTC973670.11067718679090446TruSeq Adapter, Index 2 (100% over 50bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACA967480.109973568741426No Hit
AAATAAGCGCTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC928900.10558817547020158Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC928700.1055654414459858No Hit
AAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC928000.10548587236123055Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA917800.10432643712622565Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC915530.10406840595137651No Hit
GGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTCT912220.1036921578506053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCCAAGATCGGAAGAGCACACGTC902390.1025747805603996No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA884040.10048893383860158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)