FastQCFastQC Report
Sat 30 Sept 2023
SRR18220726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18220726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154139175
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC27068861.756131106838998No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA13693340.888375067532313No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA12219860.7927809396929756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12182540.7903597511794129No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC11896190.7717823843289676No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA11201670.7267244034490259No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC10516720.6822872900416134No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG8615830.5589643255843298No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC8222690.5334588043565175No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC8076010.5239427290304363No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT7033990.45634018736638493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA6883040.4465470896675034No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG6429070.4170951349648783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT6037640.39170055243905383No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC5879830.3814624024035421No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC5745350.37273781957117647No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA5676500.3682710770963968No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG5576000.36175099548833056No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC5547330.35989098812809917No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC5430050.3522822799590046No Hit
TCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA5410590.3510197845550944Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5214400.33829167698607443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA4888290.3171348231233234No Hit
ACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT4828250.3132396420313006Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC4439490.28801827958401877TruSeq Adapter, Index 6 (100% over 50bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA4410820.28615827222378737No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG4334660.28121728301711746No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG4310570.2796544097241989No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC4302130.2791068526219892No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG4275620.2773869783590057No Hit
TGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTG3828670.24839045622243663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC3803730.24677243796069362No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG3675970.23848382476421068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC3619180.23479949208239892No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3581070.23232705118604666No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA3424660.22217972815801046No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT3380970.2193452767604342No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA3217800.20875938903915894No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3163570.2052411400281596No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3163510.20523724744212496No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3008810.19520086311607676No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2993730.19422252649269725No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACAC2910230.18880534426111986No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2776610.1801365551619178No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT2740600.17780035477677883No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT2703260.17537786873453812No Hit
ACTGGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC2622370.1701300139954687TruSeq Adapter, Index 6 (100% over 46bp)
TCACCTCTAGCATTACAAGTATTAGAGGCAGATCGGAAGAGCACACGTCT2614730.16963435804038784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG2580500.16741363770761067TruSeq Adapter, Index 6 (100% over 50bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2580160.1673915797200809No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA2485890.16127567829528086No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2473340.1604614790496965TruSeq Adapter, Index 6 (100% over 49bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC2471110.16031680460207473No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2451930.1590724745996597Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2413420.15657408312974297No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT2402430.15586109112105992Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT2379270.15435855291167866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGA2329380.15112186762385357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2300030.14921774428856258No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2225250.14436628456069003No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA2222850.1442105811193034Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA2204400.14301361091364348No Hit
TGACTGGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG2201150.14280276250343238TruSeq Adapter, Index 6 (97% over 45bp)
TGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2166070.14052689720183076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2103740.13648314907615147No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2096090.1359868443567315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2018030.13092258992563052No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC2005440.1301057956226897No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC1973730.1280485639033685No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1943830.1261087585294264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1930370.12523552172898292Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1918300.12445246317167587No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1888940.12254769107204577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT1796400.11654402587791196TruSeq Adapter, Index 6 (97% over 48bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1714080.11120339783834965Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1706770.11072915110645948No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1701160.11036519431221817Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCACAC1692440.10979947180851332No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGT1692170.10978195517135732No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCTG1683020.1091883358010707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1658220.10757940024007524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA1658130.10757356136102325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCT1657050.10750349481239924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCT1618510.10500315704946521TruSeq Adapter, Index 6 (100% over 50bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACG1601050.10387041451337728No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC1599980.10380099672909239No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1599200.10375039311064174No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAA1574090.10212134585513384Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1553760.10080240795372106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)