FastQCFastQC Report
Fri 30 Jun 2023
SRR1822445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51766593
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT14682052.836201717968961No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA10087811.9487104357051275No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA9225821.7821957106584165No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT6825411.3184970469275425No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT6791621.3119696712511097No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6689101.292165393229568No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4582720.8852659088458845No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA4540210.8770540491239206No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA3957310.7644524722729966No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA3887510.7509688729177135No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA3735720.7216468736893695No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3196780.6175372599854118No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2612460.5046613749527615No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG2591010.5005177760104862No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA2468900.4769292041297754No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG2229500.4306831627880166No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG2154800.41625300703100165No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG2111330.40785569952420864No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1964530.3794976424274242No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1936930.37416601861358734No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1788640.34552013110076607No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1681410.3248059998849065No Hit
AATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGC1632060.3152728246960351No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1436730.2775399957265876No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1415600.2734582127125886No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1405330.2714743077644689No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1392330.26896303567824137No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1372040.2650435194759678No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1336350.258149111725394No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA1302530.2516159408056852No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1254110.24226241815836713No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA1240390.23961206023351778No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1226310.23689215938935754No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1223090.2362701366110766No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1192120.2302875138025792No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1126820.21767320093868261Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1085970.20978201134465232No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1076620.20797582719032717No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1057720.2043248239265041No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG1049490.20273499552114624No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA994530.1921181098396798No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG986740.19061327833570196No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG929020.17946323027285185No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC922360.1781766862655999No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT911090.1759996065416165No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT908010.17540462823195646No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA889240.17177873768899568No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC848760.16395902276203497No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG841960.16264543428616213No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC818540.15812128103543535No Hit
GTCTAGTCGGTTATGGCATCTGCTGTAGGCACCATCAATAGATCGGAAGA815460.15752630272577528No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG799080.15436209989712865No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT783500.1513524368891729No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG766260.14802210375328353No Hit
AGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAG752910.14544322049550373No Hit
TTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAAG747950.14448507360722002No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG743460.1436177188635922No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAATA742830.1434960187547981No Hit
TTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATAGATCG717180.13854108575389537No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA705510.13628673611956654No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA696920.13462736479489776No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG696210.13449021070403455No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT693460.1339589800704095No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAAT691350.13355138129333718No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA687690.13284436161367622No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT659720.12744126313276982No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG637820.12321073554135578No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG629140.12153397848685928No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA613160.11844704556855808No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT611690.1181630786480385No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA610450.11792354192596759No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG605910.1170465284435466No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG599400.1157889606526742No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGATC597360.11539488410991235No Hit
GCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAAG584920.1129917898981685No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA580360.11211091292023025No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAA573860.11085527687711648No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA564370.10902204825417042No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA561970.10855842879209765No Hit
AAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGATCG559670.10811412680761123No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAACCTGTAGGCACCATCAAT554100.10703814330605067No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT553730.10696666863898112No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC551610.10655713811415018No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA541380.104580960157065No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA538460.10401688981154314No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG521820.10080246154117194No Hit
AAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATCGGAA521590.10075803134272328No Hit
GCCCAGTTGGTTAAGGCACCGTCTGTAGGCACCATCAATAGATCGGAAGA520430.10053394860272144No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC519230.10030213887168506No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA519040.10026543566427097No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG519010.10025964042099506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)