FastQCFastQC Report
Fri 30 Jun 2023
SRR1822446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69018133
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG26348673.8176445601621825No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT21493113.114125095212297No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT18340432.657334993399488No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA14401992.0866965497313585No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA12710791.8416594954836No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA12180921.764886917471384No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA10292681.4913008440839743No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA9709341.4067810266615006No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT6957251.008032193510653No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5202620.7538048008340069No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA5161750.7478831686159926No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG4772450.6914777019540648No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA4529570.6562869499816809No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC4001010.5797041771616743No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG3831320.5551178847448685No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG3363360.4873154131827936No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA3328250.4822283442526618No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA3223880.46710623134358037No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2968520.43010725891411755No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA2885330.41805390476152116No Hit
TTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA2692120.3900598122525279No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2646360.3834296705765715No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA2589910.37525066057640244No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA2514470.3643201997364953No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG2443550.3540446392544406No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2235450.32389314269048686No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2191880.31758030893127753No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2029150.2940024471539965No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1889970.27383673215269383No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1858820.2693234254829814No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1753950.25412886784404903No Hit
TAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1741840.2523742564870597No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1723870.24977059289621756No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1719570.24914756821949965No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1712170.24807538621770603No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1652160.23938056974099836No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA1585330.22969760714912413No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA1575730.22830666833598642No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1559500.22595511240502553No Hit
TTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAAG1524870.22093759041555064No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1469830.21296287455356117No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC1462070.21183853234627487No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1408400.20406231504407688No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1400410.20290464826105917No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1390030.20140069566935404No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1289810.18687987401803524No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG1265450.18335036677969832No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1177400.17059284985295095No Hit
CTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1131070.16388012118496453No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1128060.163444003911262No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1121060.1624297776933491No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1104930.16009271070835834No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG1096180.1588249279359672No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1076440.1559648100014528No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG1062040.15387840178174628No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1035070.14997073305358752No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1007710.14600655743614507No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1004860.14559362247599483No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAG1001860.14515895409688928No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG996700.1444113244848278No Hit
AGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA990130.14345940073458668No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA974800.1412382453173574No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC965930.13995307580980204No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA952740.13804198383633473No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA922490.13365907768035393No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC918750.13311719110106904No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC913050.13229132118076853No Hit
GTCTAGTCGGTTATGGCATCTGCTGTAGGCACCATCAATAGATCGGAAGA867510.12569305518594656No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC864650.1252786713311993No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT838510.1214912608545931No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAA824080.1194005059510955No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG818450.1185847782929741No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA806090.11679394457105932No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG805820.11675482441693982No Hit
GCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGA800490.11598256359672898No Hit
TCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC797990.11562033994747438No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA764590.11078103199343281No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGATC759170.1099957311218488No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA757600.10976825467011692No Hit
GCCCAGTTGGTTAAGGCACCGTCTGTAGGCACCATCAATAGATCGGAAGA757520.10975666351334076No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA756410.1095958362130717Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT744570.1078803450102019No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG737590.10686901658148301No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT731030.10591854172583891No Hit
TGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAGA725550.10512454748667281No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG720490.10439140682058148No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA720240.10435518445565603No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG707100.10245133695517379No Hit
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG706740.10239917674968113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)