FastQCFastQC Report
Fri 30 Jun 2023
SRR1822447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59666579
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG9242521.549027974270152No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG8831461.4801351356175456No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA7521311.260556600706067No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA7483981.2543001669326475No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG5426190.9094186546207049No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5221640.8751364813457798No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA5015170.8405325198885627No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4940480.8280146244013755No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA4867610.8158017572953193No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3911700.6555931420167393No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3674480.6158355417024998No Hit
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG3579430.599905350698923No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG3235430.5422516347049158No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA2981420.49968006377573615No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA2913840.4883537901510995No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA2747940.4605492800249198No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA2675510.44841015604397233No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA2651030.44430735671974764No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA2618290.43882019781962023No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGC2544400.4264363807417214No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGGAA2450580.41071233529242557No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2435960.40826205236268026No Hit
TAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2419110.4054380258670436No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA2392000.40089444377228334No Hit
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGA2330470.39058213811789005No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2277990.38178659446857177No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2268250.3801541898354856No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG2145970.35966030497575535No Hit
ATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATCGGA2071410.34716419723007746No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2068840.34673347034023855No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1893510.31734851096457195No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1659110.278063537043074No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1611040.2700071006249579No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1568860.26293781649522757No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1512780.2535389200041115No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1495120.2505791391190703No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1466180.24572885266306285No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1451630.24329030159413026No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1429140.23952102231301042No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1419010.2378232544553962No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG1392380.23336011940620896No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1369300.22949195729823893No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1352950.2267517298084075No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1352080.22660591953830633No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCAATAGA1324690.22201541000029515No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1296600.21730758185415658No Hit
TAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1288480.21594668599987946No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAA1281380.214756740117445No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1280120.2145455666228158No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1261580.21143829948755735No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1235980.2071477903903289No Hit
AGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGATCGGAA1227320.2056963916097821No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGG1184790.1985684481759881No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG1174520.1968472165967484No Hit
CGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAG1119250.18758407449503683No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1112810.18650474330026529No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1109170.18589468653800312No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1102350.18475166809881963No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1082830.18148015491218292No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1077630.1806086452518084No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA1077360.18056339378867356No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA1010620.1693779024937897No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA1006160.16863041536200693No Hit
AGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT880650.14759518892477477No Hit
TATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGGA871910.14613038230329914No Hit
CTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA867650.14541641477383846No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA811410.1359907026008647Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA783740.13135326561960256No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG776810.1301918113991419No Hit
AAGAGGTGCACAATCGACCGACTGTAGGCACCATCAATAGATCGGAAGAG766680.12849404354152766No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG756410.12677281196228796No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTCTGTAGGCACCATCAATAGA732380.12274543174328799No Hit
AGTTGGTGGAGTGATTTGTCTGCTGTAGGCACCATCAATAGATCGGAAGA729850.12232140877391345No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG713150.11952252198001834No Hit
AAGAGGTGCACAATCGACCGATCTGTAGGCACCATCAATAGATCGGAAGA710720.11911525881180485No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA708680.11877335886811946No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT701610.11758844092603332No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT696610.11675045086798087No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC694710.11643201464592096No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA689360.11553536528380486No Hit
AGTTGGTGGAGTGATTTGTCTGCTTCTGTAGGCACCATCAATAGATCGGA687330.11519514132023558No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA685710.1149236325414266No Hit
AATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATCGGAAGA678000.11363145187190975No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGG672470.11270463486770374No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA665780.1115834041700296No Hit
TTGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAG664550.1113772586157487No Hit
AATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCGGAAGAG648430.10867557866858767No Hit
TAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAG646640.10837557822780489No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA643200.1077990410678648No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA638540.10701803433375995No Hit
TTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA632780.10605266978688355No Hit
ACGTGAAATTGTTGAAAGGGACTGTAGGCACCATCAATAGATCGGAAGAG618970.10373814124654272No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG613010.10273925709734424No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATCG611250.10244428459690977No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA606410.10163311022071501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)