FastQCFastQC Report
Fri 30 Jun 2023
SRR1822448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73184558
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG20274022.7702592669890826No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA19028262.6000375653016854No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT17073712.3329661975959466No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA12339911.686135755578383No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA10968081.498687742296674No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG8425701.1512947854382067No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA7950971.086427276092861No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA7792651.0647942971794677No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT7664501.047283772623181No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6718770.9180584242921846No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA4925800.6730654846613954No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4825830.6594054991764793No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA4797450.6555276319356879No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT4474100.6113448140248384No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA4441030.6068261012111326No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3745710.5118169874032715No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3685030.5035256208010439No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA3656720.49965731841954963No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG3319460.4535738263254934No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA3011720.4115239720379264No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2937080.40132509921013665No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG2931820.40060636835437335No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG2747160.37537426952827946No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2453860.3352975090728839No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA2442060.3336851470770651No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA2420240.3307036437932712No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2267560.30984131925754066No Hit
TAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2130140.29106413404860626No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT2108300.28807989794787037No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG2086050.2850396390998221No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG2048480.27990604247415146No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA2037980.2784713135795669No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA1938970.26494250330786995No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1925920.263159340253172No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1869930.25550881922385865No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1814940.24799493904164865No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1762540.24083495865343615No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA1730300.23642965774282604No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1697170.23190274647829395No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG1650240.22549019152373648No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1599260.21852424113841065No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1532520.20940483100273694No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1483170.20266160519818946No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1453280.19857741027827208No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT1436410.19627227918763956No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG1418550.19383187365837476No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1393840.1904554783264524No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG1390550.1900059299394826No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG1333010.18214361559715916No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT1230780.16817482179778964No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGATC1229140.16795073080854026No Hit
CTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1202760.1643461452619554No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1201060.16411385582187982No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1187290.16223231135726746No Hit
TTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1186560.16213256353888206No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA1163400.15896796151996984No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1133000.15481407976802977No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA1118670.15285601642903957No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1067950.14592559266396063No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG1057710.1445263903896229No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA1054160.14404131538240622No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1047340.14310942480516176No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG1020340.1394201219333729No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA982280.1342195712926216No Hit
AGGTCCAGACACAATAAGGATTGACAGACTGTAGGCACCATCAATAGATC950840.1299235830596941No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC947900.1295218589692104No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT947790.12950682847602907No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG940250.12847655648887024No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC934510.12769223802649735No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA923610.12620285279307147No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA907140.12395237804128023No Hit
CTTTGAACGCACATTGCGCCCCTTGGCTGTAGGCACCATCAATAGATCGG902860.12336755521567815No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT882130.1205349904552269No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT880770.12034915890316643No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT873210.11931615409906554No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGG860500.1175794489323827No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA838940.11463347226883572No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT825720.11282708027013022No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA823980.11258932519617047No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG819980.11204276180775731No Hit
CACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGAA813760.11119285573877484No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG811950.11094553580551789No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA800580.10939192937395345No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAA800220.10934273866899627No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA799630.10926212056920533No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCCTGTAGGCACCATCAATAGA799450.10923752521672674No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG796320.10880983936529343No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG789350.10785745266098348No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG783940.10711822567815467No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC779490.10651017390854503No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCTGTAGGCACCATCAATAGATCGG748940.10233579602953946No Hit
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG740740.10121534108329246No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC736370.10061822058145109No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT736140.10058679318661731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)