FastQCFastQC Report
Fri 30 Jun 2023
SRR1822453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23126999
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA14563066.296995126778014No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA13419485.8025167900080765No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT7872363.4039695336173965No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT5011612.166995380593911No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG4520161.9544948309116974No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA3522121.5229472704175755No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA2398331.0370260317821607No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT2185990.9452112658456033No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2117090.9154192465697776No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT2070040.8950750592413655No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1778210.768889210398634No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1678510.7257794234349213No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1486770.6428719956272753No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1359050.5876464992280235No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1351310.5842997615038596No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1137640.4919099101444161No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG949520.41056775243515164No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC925040.39998272149361014No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA858520.3712198024482122No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG822150.35549359430508043No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG769770.3328447413345761No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA761600.32931207373684757No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA682590.29514854045697847No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT677880.2931119597488632No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG654010.2827906897907506No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG618790.26756173596064065No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA615200.2660094377139031No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA586960.25379860136630783No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG542690.23465647229024394No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT536690.23206210196143476No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA524410.22675229068847194No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGA521130.22533403490872292No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA492880.2131188746105796No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG492270.21285511362715068No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA482230.2085138672769433No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA475720.2056989754701853No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA443280.19167207989242355No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG412240.17825053739138397No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA403330.17439789745310233No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA384800.16638561708762992No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA375800.16249406159441612No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGTAGGCACCATCAATAGATC362490.15673888341500772No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA358990.15522550072320235No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA356690.15423099209715882No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT353750.1529597506360423No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG344400.148916856873648No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT333770.14432049744110767No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA322860.13960306739322295No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGATC321390.1389674466626647No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA318270.13761837409168393No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA310190.13412462204888753No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA308260.13329009959312058No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC301890.13053574309403482No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG299790.12962771347895158No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG297670.12871103596277234No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA293400.1268647090787698No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCG292590.12651446908438055No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT280150.12113547460264949No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAA275500.11912483759782236No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC272580.11786224403780188No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA267950.11586025493407077No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT266140.11507761988488001No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG262050.11330912411074173No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG248770.10756691778297738No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG245790.1062783805196688No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA238960.10332512229537434No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT237200.10256410699892363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)