FastQCFastQC Report
Fri 30 Jun 2023
SRR1822461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28760133
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA26610309.252495459600274No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT24568018.542384000797215No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT16366475.690679525021667No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA15543955.404686410872996No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA10201973.547261064474215No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC6576362.2866236397446427No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA6059302.1068400483405276No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT5004511.7400858334000056No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG4026151.3999065998755986No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA3813001.325793590732004No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3128601.0878252892641351Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA3091441.0749046257887611No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2971811.0333088515272166No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC2643690.9192203666095702No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1795100.6241626212229269No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1550740.5391977846555855No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1456120.506298075881638No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1269280.4413331468251555No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1258360.4375362241892275No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1070630.3722618389838461No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1006230.3498697311309374No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG941290.3272898633674608No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA926180.32203606290694137No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG897070.3119144129131809No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA891360.30992902571069475No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA879230.3057113817936795No Hit
TGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATCG837240.29111131022933723No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC801270.2786044139642887No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA786580.2734966489897665No Hit
GCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAAG748850.26037779449768195No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCATCATCAATAGATCGGA719660.2502283282208744No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATTGGA685390.23831252797057648No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT677890.23570475143491165No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT673690.23424439657493934No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG673550.23419571807960696No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG612500.21296841707929515No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT593140.20623687658189896No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG590720.20539543401972446No Hit
TGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAGA556460.19348311080480748No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG545140.189547106753644No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA544070.18917506396788916No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC534960.18600748473590162No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA513680.17860835344537523No Hit
TAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAG513670.17860487640999434No Hit
ATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA499410.17364662395685027No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA494690.17200546325707186No Hit
AATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGC492100.1711049110934223No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAAT461620.16050690725248037No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA454450.1580138728843848No Hit
GCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA454140.1579060847875773No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG443820.1543177842745025No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT442910.15400137405484182No Hit
CTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGA436640.15182127287102604No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA428840.14910918527393457No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC420070.14605982524489716No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAATA414990.14429349127140684No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA394910.13731160422658684No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACTATCAATAGATCGGA387970.13489854167225165No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGTACCATCAATAGATCGGA376610.13094862947956465No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAACCTGTAGGCACCATCAAT371380.12913013997536105No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATTAATAGATCGGA371250.1290849385154095No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG370480.12881720679108125No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT363980.12655713379350506No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA360140.12522195220724466No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG353700.12298274142195377No Hit
AATACTGAATATCTAAATATTATTATTACTTCTGTAGGCACCATCAATAG338480.11769069357224461No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA330270.11483604752453681No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAAT317270.11031590152938443No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCATCATCAATA312340.10860172308660741No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGTACCATCAAT311060.10815666255785397No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG308880.10739866884482072No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC308830.10738128366791627No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA307450.10690145278535394No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC307010.10674846322859494No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAAT304590.10590702066642042No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA292180.10159201975874033No Hit
GCATGGAATAATAGAATCTGTAGGTACCATCAATAGATCGGAAGAGCACA289660.10071580684275695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)