FastQCFastQC Report
Fri 30 Jun 2023
SRR1822462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26913484
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA20988367.798455227870163No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT7322522.7207625738830394No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6494502.4131026663065995No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT5267801.957308834486089No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA4937301.834507936616456No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG4301691.5983400736968874No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT3774771.4025571717136287No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA3000661.1149281155869675No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2487830.9243805075552463No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1970120.7320196820300188No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1822280.6770881094398629No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1729080.6424586278015882No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA1555740.5780522506859387No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1538200.5715350714162462No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1520180.5648395428848975No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1406310.5225298961665461No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG1294360.48093364649482023No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1292310.48017194652316286No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1210720.44985628765120117No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1123620.41749332787980925No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG1029960.3826929281991138No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG864550.321233029510412No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG862870.3206088070946147No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA831710.3090309675254233No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC799310.2969923923636197No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA762100.28316660897563467No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG752390.2795587520367114No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA750330.27879333645543625No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG747750.27783470917403336No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA669410.24872662342786986No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT634070.23559565903842106No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG616340.2290078831859896No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA566040.21031836680825122No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG562230.20890271954385392No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG561220.20852744297245202No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT542880.2017130149333323No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAA537390.1996731452531378No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA527830.19612102245848215No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA524050.19471652202293838No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA504520.18745993643929565No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT494490.18373317999260147No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG490610.18229152346087932No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT486020.18058605864629046No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA447840.16639986112537494No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG446700.1659762816289411No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC435450.16179622080887038No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG431120.16018736184434537No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA426850.15860079653752743No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA419670.15593298883191786No Hit
GTCTAGTCGGTTATGGCATCTGCTGTAGGCACCATCAATAGATCGGAAGA418250.1554053722661845No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA408330.15171948752528658No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA407560.15143338558471284No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG403810.15004003197802263No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG393970.14638387211406745No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA393430.14618322919470406No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT390260.14500538094584856No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT389810.14483817851304573No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT385930.14339652198132358No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA381830.14187312203800892No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATAGATCGGAA374810.13926476408628477No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC373000.13859223874545562No Hit
TTTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCG371360.13798287876812976No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG368960.137091132459848No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG366180.13605819298608832No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATC365020.1356271822704188No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA356960.13263240091843925No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG352040.13080432098646166No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA346870.12888335081403807No Hit
CTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG344240.12790614548454596No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT339290.12606691872371484No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA334700.12436145390912601No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC329300.12235502471549206No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA317270.11788514634522977No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT317210.11786285268752274No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA315770.11732780490255366No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA309340.11493866791828217No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC306970.11405806843885394No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG304090.11298797286891582No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACCTGTAGGCACCATCAA303820.11288765140923412No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA300880.11179526218158897No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA299200.11117103976579176No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA294690.10949529982814561No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG294390.1093838315396104No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA292940.10884506814502351Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG291780.10841405742935399No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAA290990.1081205242695446No Hit
CTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT289020.10738854917482997No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA286880.10659340871661208No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA286850.10658226188775856No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG280440.10420055612272272No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTCTGTAGGCACCATCA275000.10217926449061741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)