FastQCFastQC Report
Fri 30 Jun 2023
SRR1822464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21606439
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA220497910.205193923903888No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA17601608.146460413953452No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT4840042.240091483839609No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT4795942.219680901605304No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT4619122.137844186170613No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA2526061.1691237042809322No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA2319601.0735688560248176No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2300601.0647751811392892No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2049970.9487773528992908No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA1670830.7733018846835427No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1594780.7381040438917307No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1460570.6759883014503223No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1438820.6659218578313623No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1277480.5912496733034074No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1272550.5889679460831098No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1213180.5614900261908036No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1043090.4827681229655659No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1031300.4773114162865987No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA966800.4474592041751998No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT944120.4369623333118428No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA809660.3747308846219407No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA673950.3119209046895696No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA667320.3088523749795142No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA655100.3031966535531375No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA640060.2962357656437509No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG625180.28934892973340026No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA617800.28593328127786355No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG610450.28253151757214595No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA582640.26966035448969633No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG580730.26877635875120376No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA579260.26809600601006023No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA558430.25845536138555736No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA513770.237785597154626No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT505940.23416167745180036No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA488680.22617331805578883No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT485100.22451640457735772No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG459810.21281156047972552No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG445670.20626721506491652No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC442260.20468898183546116No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA441380.20428169584076306No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG419700.19424764997138122No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC407800.18874003254307664No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC402330.18620837982603242No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAA398500.18443576009910748No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA387570.17937708291495882No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA383860.17766000218731093No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG382100.17684543019791463No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG377570.17474883297520707No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG374880.17350383374141384No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA363280.1681350638113018No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA363250.16812117906148255No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGTACCATCAA354480.16406220386432024No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT352330.16306713012727364No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG351670.16276166563125002No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA348250.1611788041518549No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT340230.157466947700174No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC339150.15696709670668083No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAA336840.15589797097059815No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG335620.15533332447794845No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA331910.15361624375030053No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT329070.15230182076741106No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT325790.15078375478717246No Hit
GTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA325740.15076061353747372No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAA321110.14861773381536864No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA320210.148201191320791No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATTTGTAGGCACCATCAA318020.14718760458398536No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA300820.13922701468761234No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA289190.13384436000768105No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGTACCATCAA286850.13276134952177912No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA278670.12897544107106218No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA278660.12897081282112244No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA277960.12864683532533983No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA276570.12800350858371431No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG268550.12429165213203343No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG266950.12355113214167314No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT253680.11740944447162255No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA245640.11368833152006215No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA245510.11362816427084538No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG244290.11306351777819565No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC238420.11034673506356138No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT237860.11008755306693527No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA236240.10933777657669551No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT235810.10913876182928617No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA231630.10720415335446992No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA229010.10599155187025497No Hit
TTTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAA228720.10585733262200217No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA227840.10545004662730402No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT224310.10381627439857163No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA221800.10265458366369397No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC219190.10144661042941876No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG218910.10131701943110569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)