FastQCFastQC Report
Fri 30 Jun 2023
SRR1822470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45817932
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT551271112.031776117699945No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT38677288.441515867630168No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA20789674.537452716111238No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT11828792.5816944335244116No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA9494202.0721581235923088No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG8269111.804775911754376No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC7543011.6463008413387141No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA7515991.6404035869624147No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA7173201.565587901261017No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA6283821.371476128603971No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA5177461.130007351706751No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA4336350.9464307555391195No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA4180290.9123698555404027No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT3559500.7768792358415478No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG3461770.755549159224384No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2703140.5899742485103867No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG2661150.5808097144148715No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG2468740.5388152394132498No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC2249380.49093878789640705No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2241040.4891185398764833No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA2015520.4398976365847328No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1987260.4337297458121855No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1792600.39124419670446936No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1747350.3813681507930127No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1709120.3730242560925709No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA1530540.3340482499297437No Hit
TGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAGA1523490.3325095510639808No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG1425990.3112296731332178No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1416660.3091933525066125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG1385590.3024121647393426No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT1353030.29530577678625913No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG1305660.2849670299392823No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT1282210.27984894647798597No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT1282160.27983803372007277No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG1263750.27581995625642813No Hit
TGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATCG1175650.256591676813349No Hit
GCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGA1079470.23559989569149475No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1070720.23369016305668266No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC1061100.23159054843418075No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA1051720.22954331504966222No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1049370.22903041542774125No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA1048200.2287750568925721No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA940220.20520786490319992No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCATCATCAAT837890.18287381455802065No Hit
GCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA833350.18188293613950102No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT789470.17230589979486632No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA751840.16409295818938316No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGATC749430.16356696325796632No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA719940.157130618640754No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT690000.1505960592023228No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT687910.15013990592155055No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG681780.14880200180139078No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATC678210.14802283088638746No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG673170.1469228248887357No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA672200.14671111738521941No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC656590.14330415436471466No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGTAGGCACCATCAATAGATC648410.14151882717011321No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG648090.14144898551946866No Hit
CTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGA634960.13858329529145924No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAAT611050.13336481445736137No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGTACCATCAAT605280.13210548219417673No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA600660.13109714336299597No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG591590.12911756907753932No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAAT567680.12389908824344145No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG566910.12373103177157799No Hit
AGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAG564920.12329670400663215No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC562260.12271614528564931No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA548640.1197435100300904No Hit
AATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGG532050.11612265695448672No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCATCATCAATA528520.11535221624581397No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATAGA523290.11421074176809203No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA522790.1141016141889599No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGAT520910.11369129449142314No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA489280.10678788383552537No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA479440.10464025307820528No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAG464980.10148428348970442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)