FastQCFastQC Report
Fri 30 Jun 2023
SRR1822471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1822471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32672715
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT29473469.020817523122886No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA15762174.824260854967211No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT12838393.9293918488255413No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT10558423.2315710524821704No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA9918193.0356185581761417No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG5261661.6104140718027258No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA4812391.4729078988385262No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4154491.2715472222005426No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC3947251.2081181499609077No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA3110960.952158398835236No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA3032860.928254661420087No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG2519630.7711725211694223No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG2297800.7032779492001201No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA2271990.6953783914192623No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2207630.6756799978208116No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG2061770.6310372431553362No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1983430.607060049952996No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1881460.5758505223701184No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT1819700.5569478997995728No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1790850.548117902047626No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1380040.42238301898082237No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1375400.4209628737617917No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1339330.40992308107850844No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1315950.40276726314296196No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1286520.39375974723863627No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG1260680.38585100748437956No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1173010.3590182205549799No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1142670.34973218479088747No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1123690.34392305628718034No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1122140.3434486543282369No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA1111970.34033596534600813No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1081100.330887714718535No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGCTGTAGGCACCATCA989160.30274802690869124No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT973300.2978938236384702No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA954400.2921091803971601No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA869010.26597422344607724No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA857370.2624116177673022No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC836070.2558924166540797No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT793620.2428999242946293No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG781220.23910470862308197No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG768950.23534928150292989No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG754390.2308929637466614No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT749980.2295432136570224No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG731600.22391772462129333No Hit
TGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAGA727300.2226016417674503No Hit
GCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATA712790.21816062730018No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG694370.21252289563325238No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC675770.20683007212593138No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG665730.20375717169509788No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT665700.203747989721699No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG651410.19937430972602066No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA651150.19929473262323014No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT646130.19775828240781337No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA629440.1926500445402226No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC617790.1890843782036479No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA610360.18681030945851912No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT599090.18336094811833054No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA590960.1808726333272273No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA587660.1798626162533478No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA580370.1776313967174139No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCG550540.16850145450110285No Hit
AGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAG533210.16319733453433544No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG523130.1601121914723034No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA516970.1582268262677283No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC488780.14959883193055734No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT487560.14922543167900187No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG487520.14921318904780334No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT480110.14694524161827385No Hit
TGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATCG468600.143422424490894No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA467120.14296944713654802Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGA439490.13451284963615665No Hit
GCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGA433330.13262748443158154No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA417330.12773043195216557No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCATCATCAA412930.12638374252032622No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATAGATC405320.12405458193480402No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG400970.12272319579196281No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA399730.12234367422480807No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAAT392080.12000227100808733No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA390410.11949114115554829No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC390160.11941462471055742No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC387600.11863109631385087No Hit
AGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA380080.11632948164852538No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT377610.11557349917201555No Hit
CTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGA376170.11513276444886811No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG370770.11348000923706524No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAAT356150.10900532753399894No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG354570.1085217436016566No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC351520.10758824297276795No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT351320.10752702981677525No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG349230.10688735233665154No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCAT347640.10640070774650959No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGTACCATCAAT338690.1036614190158363No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAAT337440.10327883679088193No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC335770.10276770693834289No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA330750.10123125672292614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)