FastQCFastQC Report
Sat 23 Sept 2023
SRR18277492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18277492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145908345
Total Bases10.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4199180.2877957391676261TruSeq Adapter, Index 2 (100% over 48bp)
CTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3600600.24677135499001102TruSeq Adapter, Index 2 (100% over 48bp)
ACTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3507170.2403680200745201TruSeq Adapter, Index 2 (100% over 48bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA3226550.22113539838999613TruSeq Adapter, Index 2 (100% over 47bp)
ATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3221370.22078038099877015TruSeq Adapter, Index 2 (100% over 48bp)
CATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT3206200.21974068721017978TruSeq Adapter, Index 2 (100% over 43bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3080190.21110444368346443TruSeq Adapter, Index 2 (100% over 48bp)
AATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT3065100.21007023278894707TruSeq Adapter, Index 2 (100% over 43bp)
GATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2998270.20548996015272467TruSeq Adapter, Index 2 (100% over 43bp)
TCTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2986340.20467232357409026TruSeq Adapter, Index 2 (100% over 48bp)
GCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2966410.20330639758815716TruSeq Adapter, Index 2 (100% over 43bp)
TATCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2873360.1969291064195129TruSeq Adapter, Index 2 (100% over 43bp)
CCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2800180.19191362906624704TruSeq Adapter, Index 2 (100% over 43bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2659590.18227812809472962TruSeq Adapter, Index 2 (98% over 50bp)
TCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2606860.17866421553887132TruSeq Adapter, Index 2 (100% over 43bp)
CGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2509650.1720018138784317TruSeq Adapter, Index 2 (100% over 48bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA2484710.17029252165117767TruSeq Adapter, Index 2 (100% over 47bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2412580.16534900728261978TruSeq Adapter, Index 2 (98% over 50bp)
CCCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2381090.16319080310313985TruSeq Adapter, Index 2 (100% over 48bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2378400.16300644078993562TruSeq Adapter, Index 2 (98% over 50bp)
TTTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2321080.15907794718663967TruSeq Adapter, Index 2 (100% over 43bp)
ATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2317960.15886411431779313TruSeq Adapter, Index 2 (100% over 43bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2288290.15683064597847368TruSeq Adapter, Index 2 (98% over 50bp)
CGTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2287890.15680323150810874TruSeq Adapter, Index 2 (100% over 43bp)
ACCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2257760.1547382365278696TruSeq Adapter, Index 2 (98% over 50bp)
GTTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2193530.1503361579490193TruSeq Adapter, Index 2 (100% over 43bp)
AGTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2170250.14874063577377977TruSeq Adapter, Index 2 (100% over 43bp)
GGTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2139140.14660847534114652TruSeq Adapter, Index 2 (100% over 43bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA2078100.14242502716345662TruSeq Adapter, Index 2 (100% over 47bp)
TGTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2069380.14182739170950093TruSeq Adapter, Index 2 (100% over 43bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA2065470.14155941526168364TruSeq Adapter, Index 2 (100% over 47bp)
CTTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT2030110.1391359760814229TruSeq Adapter, Index 2 (100% over 43bp)
TGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1920400.13161687222207888TruSeq Adapter, Index 2 (100% over 48bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1871130.12824009483487733TruSeq Adapter, Index 2 (98% over 50bp)
TCCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1859840.12746632140882688TruSeq Adapter, Index 2 (100% over 48bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1776030.12172230450561276TruSeq Adapter, Index 2 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1759580.12059488441185458TruSeq Adapter, Index 2 (100% over 50bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1747990.11980055013303044TruSeq Adapter, Index 2 (98% over 50bp)
ACTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT1648300.11296817875632816TruSeq Adapter, Index 2 (100% over 43bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA1611840.11046934978256384TruSeq Adapter, Index 2 (100% over 47bp)
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA1484470.1017398970566077TruSeq Adapter, Index 2 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)