FastQCFastQC Report
Thu 21 Sept 2023
SRR18327307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18327307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132712598
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length20-50
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC103360217.78827417725633No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG89453306.740377428222752No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC18482331.392658291566261No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCCTT18115511.3650181122970708No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG16818791.2673092271164792No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC15928891.200254553075662No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC15484911.1668003063281152No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT15193971.1448777455174226No Hit
CGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG14759571.1121453594028805No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT14724191.109479448213349No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCCT13670681.0300966303138757No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC12132230.9141731970313776No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC11615680.8752507429626237No Hit
TGGGAATACCGGGTGCTGTAGGCTT11577220.8723527513190572No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT10650500.8025236609413674No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC9532270.7182641394752892No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT9473630.7138455687530132No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC9357570.7051003552805137No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG9313850.7018060184459655No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA7977680.6011245443330104No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGC6791620.5117539783223896No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC5798660.43693365116701277No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT5785740.43596011887281416No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC5490040.41367888826952204No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC5287420.39841130982907896No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC5226320.3938073761467619No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTT5119910.38578929786304084No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC4736050.3568651410169817No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC4730440.35644242304713225No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGC4649990.35038045144742025No Hit
ATTGATCATCGACACTTCGAACGCA4479110.33750450729628545No Hit
CGTGGCGCAATGAAGGTGAAGGCCGGC4457140.3358490502913672No Hit
CGGGCGGGTCGCGCCGTCGGGCCCGGGGG4437050.33433525278436643No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGGT4420420.3330821690341711No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG4388450.3306732040616069No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC4330570.32631189994487186No Hit
CGGCGGCGGCGGCGGCGGGA4045910.30486254213786096No Hit
CCCCGCCACGCAGTTTTATCCGGTA4000870.3014687422515834No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC3867580.2914252345508299No Hit
CCCCCGCGAGGGGGGTCTCCCCCGCGGGGG3863710.2911336269673509No Hit
CGAATACAGACCGTGAAAGCGGGGCCT3742370.28199056128793437No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3673250.27678231421556526No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3594990.2708853608607677No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGA3558510.2681365637947951No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC3543670.2670183579708085No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT3530530.2660282485013216No Hit
CCCCGCCCCTTGCCTCTCGGCGCCC3485120.26260656882024114No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT3384010.2549878497593725No Hit
ATGTAGGTAAGGGAAGTCGGC3304950.24903061576716326No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGG3167840.23869926802276903No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCC3131360.23595047095679644No Hit
CGGCGGCGGCGGCGGCGGCGGGA3043910.2293610437797322No Hit
GCCTCGGTTGGCCTCGGGTAGCCGGTCCCC3023450.2278193664779285No Hit
CGGCGGCGGCGGCGGCGGCGGG3023100.2277929936990609No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTT2995010.2256763898179433No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG2901460.218627322780615No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCC2860220.21551985592204292No Hit
CGTGGCGCAATGAAGGTGAAGGCCGG2799910.21097544936916993No Hit
TTGGAACAATGTAGGTAAGGG2727330.20550648853999526No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC2722820.2051666564465869No Hit
CGCGCGGGAGGGCGCGTGCCCCGCCGCGCG2598880.19582767869558249No Hit
CCCGCGAGGGGGGCCCGGGC2509750.1891116621799537No Hit
GCCTCGGTTGGCCTCGGATAGCCGGT2496720.18812984129811097No Hit
CCGCGAGGGGGGCCCGGGCACCCCCCTCGCGG2383610.17960691267606713No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA2220070.1672840433731845No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT2173980.1638111251503041No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCC2165560.16317667144154618No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT2159390.1627117570255086No Hit
CCCCGCGAGGGGGGTCTCCCCCGCGGGG2158810.16266805356338515No Hit
GTCTAACACGTGCGCGAGTCGGGGG2103810.15852375974133218No Hit
GCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG2052970.15469292523381992No Hit
ATTGATCATCGACACTTCGAACGCAC2031920.15310679096192512No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCG2029530.1529267025576577No Hit
AGGGCGTGAAACCGTTAAGAGG2014020.15175801169983877No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCT1976560.14893537085303687No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTT1847770.1392309417377241No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTA1826360.137617681178994No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1813380.13663962783698952No Hit
GAAAATCCGGGGGAGAGGGT1796140.13534058010076783No Hit
TTGGAACAATGTAGGTAAGGGA1781700.134252514595487No Hit
GTCTAACACGTGCGCGAGTCGGGG1735910.130802201611636No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1717380.12940595134758795No Hit
GGGTCGCGCCGTCGGGCCCGGGGG1717130.12938711364839683No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC1704540.1284384471171305No Hit
GAATGCAGCCCAAAGCGGGTGGTA1699190.1280353203544399No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCC1674410.1261681276106131No Hit
GGGGGTCTCCCCCGCGGGGGCGCGCCGGC1663760.12536564162507013No Hit
AAGGGCAGGGCGCCCTGGAATGGGTT1650860.1243936163468068No Hit
CCCCCGCGAGGGGGGTCTCCCCCGCGGG1643070.12380663364001059No Hit
TGGAACAATGTAGGTAAGGG1611660.1214398651136345No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCT1610070.12132005734677878No Hit
CCCCCGCGAGGGGGGTCTCCCCCGCGGGG1557240.117339274753705No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1548610.1166889973776265No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1500320.11305030740186399No Hit
GGGCGTGAAACCGTTAAGAGG1488520.11216116800004171No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT1482790.11172940793458055No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGC1458980.10993530546361545No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC1441350.10860687091665555No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCCTTC1430280.10777273759647144No Hit
CCTCGGCCGATCGAAAGGGAGTCGGGT1421940.10714431195145467No Hit
GGGGTCTCCCCCGCGGGGGCGCGCCGGC1387190.10452587176388484No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCG1386750.10449271741330843No Hit
GTCGTCCGACCTGGGTATAGGG1384120.10429454481781751No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCC1357100.10225856628923805No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGC1348640.10162109854860953No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCC1344190.10128578750300705No Hit
ATTGATCATCGACACTTCGAACGCACT1338720.10087361864470472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)