FastQCFastQC Report
Thu 21 Sept 2023
SRR18360994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18360994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101821715
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length15-42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCT49971654.907759607073992No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT31232223.0673437390049854No Hit
GCAGTGATGTATGCAGCTGAGGCATCCTAACG30424492.988015866752981No Hit
ACTCGGAATCGTCTGTAGACGACTTAGGT25598932.514093383714859No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC20237381.9875308523334145No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG19476141.912768803786108No Hit
AGGACGCACCTCTGGTGTACCAGTTATCGTG11792401.1581419542972733No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCC10183591.0001393121300304No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCG8846450.8688176191100297No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGT8672780.8517613359782832No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC8628250.8473880055938952No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCT7995910.785285339183297No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGC7971540.7828919400935253No Hit
TGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAG7961810.7819363482534153No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCA7207030.70780874197611No Hit
CCATTAATGCGGTACGTGAGCTGGGT6773530.6652343264891973No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACC6183010.6072388389843955No Hit
GCAGTGATGTATGCAGCTGAGGCATCCTAACA5693350.5591489006053375No Hit
CCATTAATGCGGTACGTGAGCTGGG5604540.5504267925559887No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCC5600180.5499985931291769No Hit
GCGCCGTCCGAATTGTAGTCTG5443160.5345775211112875No Hit
AAGCGTCTGTAGGTGGCTTTTCAAGTCCGC5288190.519357781392702No Hit
GACTCGGAATCGTCTGTAGACGACTTAGGT4983010.48938578573342634No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACG4775430.46899917173856287No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCC4707430.4623208320543412No Hit
ACTCGGAATCGTCTGTAGACGACTTAGGC4530550.4449492920051484No Hit
GCTCCGCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGG4295550.4218697357435003No Hit
GCTTGACTGCAAGACTCACCCGTCGAGCAGAGACG4251750.4175680992998399No Hit
CGTCTGTAGGTGGCTTTTCAAGTCCGC4022580.3950611124552361No Hit
AGCGTCTGTAGGTGGCTTTTCAAGTCCGC3878410.3809020502159092No Hit
GCGAGCGTAGTTCAATGGTAAAACATCTCCTTG3874610.38052884888061456No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC3863260.37941415541861573No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCA3538730.34754177927566826No Hit
GCAGTGATGTATGCAGCTGAGGCATCCTAAC3267730.3209266314164911No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACA3214880.315736186529563No Hit
GCCAAGGATGTTTTCATTAATCAAGAAC3195480.31383089550200566No Hit
ACTCGGAATCGTCTGTAGACGACTTAGGG3181820.31248933491249875No Hit
GCCCTTTTAACTCAGTGGTAGAGTAATGCCATG3102920.30474049666124753No Hit
CGGGCTGAATCCTTTGCAGACGACTTAAATA2967150.2914064057946775No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGC2879670.28281491821268184No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGTC2826510.27759402795366395No Hit
ACCGTGTGCCGGATTATGACTGAACGCCT2770890.27213153893548153No Hit
CGGGCTGAATCCTTTGCAGACGACTTAAAT2761370.27119657137969044No Hit
GCCCACGGGTCGTCAACTTCTTTTCTCGGAGA2726260.2677483874633225No Hit
TCTTTTGTTACGGACGATCTCGCATCAG2667900.2620168006402171No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTA2609500.25628128538200323No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCCTT2600260.25537381687197075No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCT2595500.25490633309407523No Hit
CCATTAATGCGGTACGTGAGCTGGGTT2591770.2545400065202202No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCG2546720.2501156064794234No Hit
TGTCCGATGCACTGAACTCCAGGACCGAG2533690.24883591874287325No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGC2515070.24700723219992907No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGTT2453650.24097512009103364No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCT2448160.24043594237241045No Hit
GACTTGAGAGGTGTAGGATAAGTGGGAGCCCT2351220.23091537988728633No Hit
TGCCTAAGGCTAGGCTTGCGACTGGAGTGAAGT2320210.22786986056952588No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTG2317950.2276479039859032No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGT2100300.20627230645250869No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGT2093050.20556027758911738No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGG2080640.20434148059674698No Hit
TGTCCGATGCACTGATCTCCACGGCCCGG2067000.20300188422479432No Hit
GCGGGTATAGTTTAGTGGTAAAACCCTAGCT2003220.1967379944445053No Hit
TGGGACTCGGAATCGTCTGTAGACGACTTAGGT1963350.19282232675024183No Hit
GGGGATATAGCTCAGTTGGTAGAGCTCCGCTC1962060.19269563471799703No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTGG1884180.18504697156200914No Hit
GCTAATACCCCGTAGGCTGAGGAGCAAAAGGAG1859400.18261330601237663No Hit
GCCCACGGGTCGTCAACTTCTTTTCTCGGAGAAGA1811160.1778756132716877No Hit
AGGACGCACCTCTGGTGTACCAGTTATCGTA1810250.17778624137297236No Hit
GCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGA1719790.16890208537540347No Hit
GAGAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTG1719540.1688775326559762No Hit
ACTCTACTGCGGTGACGATACTGTAGG1710970.16803586543400884No Hit
TTCCGATCCATTGAGCTCCAATACCACT1710550.167994616865371No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATACCTGG1682780.16526730079138818No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTT1600860.15722186568945534No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGC1541700.15141171016418256No Hit
AACTCTACTGCGGTGACGATACTGTAGG1516500.1489367960459122No Hit
AGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTA1501770.14749014981725658No Hit
AGAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTG1436220.1410524267834224No Hit
ACTCGGAATCGTCTGTAGACGACTTAGGA1401050.1375983502143919No Hit
AAGCGTCTGTAGGTGGCTTTTCAAGTCCGT1395140.13701792392713089No Hit
TGGGCACTGTCTCGGAGAGAGACTCGGT1357550.13332617703404426No Hit
GCGAGCGTAGTTCAATGGTAAAACATCTCCTT1349030.1324894203559624No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATACCTGGCT1348170.13240495900113253No Hit
CTCGGATTCTAACCTTGTG1344250.1320199723605127No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1343220.13191881515647227No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCG1324450.1300753969818717No Hit
GCCGCGCCGTCCGAATTGTAGTCTG1297920.1274698623962482No Hit
TGCTAATACCCCGTAGGCTGAGGAGCAAAAGG1282380.12594366535664814No Hit
TCCATTGTCGTCTAGTCCGGTCAGGATACCTGGCT1250590.12282154155427455No Hit
ATTGGTGTATTTTCTTTGTGGTAACACTT1246510.12242084117322126No Hit
CTGGAAACGGTTGCTAATACCCCGTAGGCTG1229440.12074438149072622No Hit
GCAGTGATGTATGCAGCTGAGGCATCCTAACGA1215430.11936844709402115No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGG1194700.11733253559911067No Hit
AAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTA1186670.11654390225110625No Hit
CGCGCCGTCCGAATTGTAGTCTG1164590.11437540607128842No Hit
AAAAGGACTTTGAAAAGAGAGTCAAAGAGT1127360.11071901509417711No Hit
CTCGGAATCGTCTGTAGACGACTTAGGT1119450.10994216705149781No Hit
CCATTAATGCGGTACGTGAGCTGGGC1117650.10976538747162136No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCGTT1111770.1091879075106916No Hit
CTCCGCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGG1086930.10674834930839655No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCT1083010.10636336266777673No Hit
GCAGTTAAAAAGCTCGTAGTTGGACCTT1078490.10591944950053139No Hit
CGTCTGTAGGTGGCTTTTCAAGTCCGT1056130.10372345427495501No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCA1053610.10347596286312798No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTGGC1044300.10256161959165586No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTG1043690.1025017109562533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)