FastQCFastQC Report
Sat 23 Sept 2023
SRR18402610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18402610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151536796
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACAC35214362.323815794548012No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCACA24955091.6468006885931519No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT16095421.0621459886217999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACA14674600.9683852626790393No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC12906170.8516855536525928No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGAGATCGGAAGAGCACACG7224620.4767568135728566No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCTAGATCGGAAGAGCAC6723000.4436546223400421No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCAC6439250.42492979724871577No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGTAGATCGGAAGAGCACAC6413630.4232391187682231No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACACG6350830.41909491078325295No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCAC6148640.40575227682654713No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCCCAGATCGGAAGAGCACAC5005090.33028875706201416No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGTAGATCGGAAGAGCACA4710570.31085321349938005No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTAGATCGGAAGAGCACA4563760.3011651374759171No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACG4462780.2945014094134602No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACAC4445680.29337297061500495No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACAC4311400.28451175647134574No Hit
TGTGCTCGCTGCTTGCCTTCTTGCCGCTTCTGGTTAGATCGGAAGAGCAC4232030.27927408469161513No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC4039460.26656628004725663TruSeq Adapter, Index 6 (100% over 50bp)
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACG3697980.24403181917611616No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCTAGATCGGAAGAGCACA3565960.23531974372745745No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGCAGATCGGAAGAGCACAC3363060.2219302564639152No Hit
CTTTGAGGCCGCGTGTGGCCCCTGGACTGTGTTAGATCGGAAGAGCACAC3342530.22057547000003877No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3272880.21597922658995639No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC3265030.21546120059183516TruSeq Adapter, Index 6 (100% over 42bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3205820.21155389876396755No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGAGATCGGAAGAGCACACGTCT2944550.19431254175388532Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT2900390.19139839804980435TruSeq Adapter, Index 6 (100% over 43bp)
CTACATCACGTACGTGTTTTGAACCGAGTTGAGCAGATCGGAAGAGCACA2806100.18517614692077825No Hit
AGAGTTCGATCGTCGAGGAGTACATTGGCCTAGAAGATCGGAAGAGCACA2676280.17660925073273953No Hit
CTATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACA2665940.17592690820782564No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACACGT2646750.1746605491117814No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGT2626830.17334601689744056No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCGTC2527840.16681361007527176TruSeq Adapter, Index 6 (98% over 50bp)
GAGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACA2471190.1630752441143074No Hit
TGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2426540.160128765029452Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACA2412970.15923327295371878No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACGT2408930.15896667103876208No Hit
CTGCCGCTTCACCTATGGATTTTGTGCTCGCTGCAGATCGGAAGAGCACA2306420.15220197739960134No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA2305900.1521676623016366No Hit
CGGTCGAACTTGACTATCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2272400.14995697810583247Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGCTCGCTGCTTGCCTTCTTGCCGCTTCTGGTAGATCGGAAGAGCACA2220200.14651227019475851No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCCCCAGATCGGAAGAGCACA2155390.14223542115804005No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACACG2122760.14008214876075378No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACAC2079570.13723201591249165No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAAGATCGGAAGAGCACACGTCT2061310.13602702804934586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTAGATCGGAAGAGCACAC1968540.12990508259129355No Hit
AGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACAC1939310.12797617814223813No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACGT1938150.12789962907754762No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCAC1917560.12654088317929066No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGAAGATCGGAAGAGCACAC1916660.1264814916635825No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCTAGATCGGAAGAGCACAC1904530.12568102601298234No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1884350.12434933624965912No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCAGATCGGAAGAGCACACGT1825990.12049812640884923No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA1735260.11451080171973546No Hit
AGTTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACA1643750.10847200438367458No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAAGATCGGAAGAGCACACGTC1631430.10765900052420271No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAAGATCGGAAGAGCACA1626700.10734686511386976No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAGATCGGAAGAGCAC1624860.10722544245953305No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTGAGATCGGAAGAGCACA1580180.10427698365748739No Hit
GAGTTCGATCGTCGAGGAGTACATTGGCCTAGAAGATCGGAAGAGCACAC1578410.10416018034326133No Hit
CGCTTCACCTATGGATTTTGTGCTCGCTGCTTGCAGATCGGAAGAGCACA1574070.10387378125640193No Hit
CACGTACGTGTTTTGAACCGAGTTGAGCTGTTTAAGATCGGAAGAGCACA1559530.10291427832484988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)