FastQCFastQC Report
Thu 21 Sept 2023
SRR18402611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18402611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143779824
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACAC34623352.4080812618048553No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC20868341.451409482877097TruSeq Adapter, Index 7 (100% over 42bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT18013351.2528426797907335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC12920720.8986462523420532No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC12460570.8666424574285193TruSeq Adapter, Index 7 (100% over 50bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT10757910.748221113415746TruSeq Adapter, Index 7 (100% over 43bp)
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACACG10650100.740722843004732No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCACA9368410.6515802940473763No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACA8232400.5725699038273966No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCGTC7502950.5218360818135374TruSeq Adapter, Index 7 (98% over 50bp)
CAGCTCTGGTCAAGAGGATATCATATCCATCCCAGATCGGAAGAGCACAC5787560.4025293562746328No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACG4979030.34629545797746975No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACAC4929330.3428387838338153No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCAGATCGGAAGAGCACACGT4603170.32015409895062885No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCAC4536340.3155060198154089No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGAGATCGGAAGAGCACACG4379760.3046157574932071No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGTAGATCGGAAGAGCACAC4270800.2970375036764546No Hit
CTTTGAGGCCGCGTGTGGCCCCTGGACTGTGTTAGATCGGAAGAGCACAC4100020.28515962017035157No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGT3883390.2700928330528489No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACACG3773230.262431118290978No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACGT3686760.25641706168731987No Hit
TGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3645780.25356687041152587Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACG3416640.2376300029411637No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGTC3383850.23534943261580288No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACGT3194090.22215147516107683No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT2987370.20777393634867714TruSeq Adapter, Index 7 (100% over 43bp)
TGCCGCTTCACCTATGGATTTTGTGCTCGAGATCGGAAGAGCACACGTCT2760750.19201233686306363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT2758720.19187114876423828TruSeq Adapter, Index 7 (100% over 43bp)
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCTAGATCGGAAGAGCAC2650300.18433045237278908No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCG2641520.18371979645767267TruSeq Adapter, Index 7 (97% over 42bp)
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACAC2529610.17593636781750407No Hit
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2296690.15973659837001886Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG2199800.1529978225595825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCAC2183000.15182936932792462No Hit
TTTCTTTAGACTGTTGCGAACTTGGCACCTCCAAGATCGGAAGAGCACAC2146700.14930467573809242No Hit
AGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2124770.1477794269660533Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2057770.14311952419694157No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA2055280.14294634273582085No Hit
TACCGTCTACCTGCCGGACACTGAACCCTGGGTGGTAGATCGGAAGAGCA2046520.14233707783645638No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACAC1977250.13751929477949565No Hit
GGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1933690.134489662471697Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGCTCTGGTCAAGAGGATATCATATCCATCCAGATCGGAAGAGCACACG1892210.13160469580210363No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGCAGATCGGAAGAGCACAC1847810.1285166408327221No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTAGATCGGAAGAGCACA1808050.1257513015177985No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACA1779270.12374962985070839No Hit
GAGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACA1748570.12161442067142884No Hit
CTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1733940.12059689264886012TruSeq Adapter, Index 7 (100% over 41bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG1729600.12029504223068181No Hit
AGAGTTCGATCGTCGAGGAGTACATTGGCCTAGAAGATCGGAAGAGCACA1712610.1191133743493802No Hit
TGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACT1670760.11620267388837532Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAAGATCGGAAGAGCACACGTCT1614590.11229600614895731Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1612440.11214647195562015TruSeq Adapter, Index 7 (100% over 41bp)
CGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG1590940.11065113002224845TruSeq Adapter, Index 7 (100% over 45bp)
TGTGCTGACTGCCTTGCTTTGTTTGCAATCTGGGAGATCGGAAGAGCACA1557700.10833926184246824No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1549300.10775503522663932No Hit
GAGTTCGATCGTCGAGGAGTACATTGGCCTAGAAGATCGGAAGAGCACAC1489200.10357503289195848No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT1471570.10234885250659369TruSeq Adapter, Index 7 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)