FastQCFastQC Report
Sat 23 Sept 2023
SRR18402614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18402614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123914429
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC21204851.711249462320486TruSeq Adapter, Index 11 (100% over 50bp)
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACAC17380911.4026542461814515No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGCCGTC14463021.1671780370306997TruSeq Adapter, Index 11 (98% over 50bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA12693081.0243423709760224No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCACA11660560.9410171272305988No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCAC9250010.7464836883523871No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCTAGATCGGAAGAGCAC8679230.7004212560266084No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACA6969760.5624655704946193No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGAGATCGGAAGAGCACACG5565860.44916964431962964No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGTAGATCGGAAGAGCACAC5044100.40706316776071333No Hit
CTTTGAGGCCGCGTGTGGCCCCTGGACTGTGTTAGATCGGAAGAGCACAC4660290.37608937374032525No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACACG4384670.35384660490183917No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGAGATCGGAAGAGCACACGTCT4134520.3336592867647399Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTCTGGTCAAGAGGATATCATATCCATCCCAGATCGGAAGAGCACAC3522740.2842881195054371No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGAGATCGGAAGAGCACACGT3489540.2816088512178029No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGTAGATCGGAAGAGCACA3489150.2815773778855084No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACAC3475460.28047258322111945No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGCAGATCGGAAGAGCACAC3145030.2538066006824758No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACG3079730.2485368350444483No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCTAGATCGGAAGAGCACA2879850.2324063487392578No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACAC2841690.2293268042255192No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGT2185500.17637171212724548No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC2123630.17137875041170547TruSeq Adapter, Index 11 (100% over 42bp)
AGAGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCAC2114480.17064033761556532No Hit
GAGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACA2114100.1706096712917912No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACA2087400.16845495854239864No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTAGATCGGAAGAGCACA2082910.16809261171675174No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACG1964190.15851180656289834No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG1948840.1572730484841277No Hit
TGTGCTCGCTGCTTGCCTTCTTGCCGCTTCTGGTTAGATCGGAAGAGCAC1900430.15336632023700808No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACGT1802770.1454850750270576No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1767390.14262987888198234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCTAGGTTCTCCGTCCTACTTTTCCCACATTGGAGATCGGAAGAGCAC1692670.13659991121776463No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACGT1686530.1361044079862564No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCAGATCGGAAGAGCACACGT1680760.1356387640700019No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACACG1657610.13377053934534128No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGTC1628650.1314334426703447No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAAGATCGGAAGAGCAC1622960.1309742548222532No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT1618640.1306256271414526TruSeq Adapter, Index 11 (100% over 43bp)
AGAGTTCGATCGTCGAGGAGTACATTGGCCTAGAAGATCGGAAGAGCACA1606860.12967497110445467No Hit
AGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACAC1569440.1266551452212236No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCA1553690.12538410680163808No Hit
TGTGCTCGCTGCTTGCCTTCTTGCCGCTTCTGGTAGATCGGAAGAGCACA1544110.12461099263912197No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1535010.12387661488558366No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCCTAGATCGGAAGAGCACAC1435480.11584445908232364No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1410870.11385841111368879TruSeq Adapter, Index 11 (100% over 49bp)
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACAC1390460.11221130672361004No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGAAGATCGGAAGAGCACAC1343100.10838931437112945No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1337160.10790995131002862No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCAC1324240.10686729630170833No Hit
ATGTACACGGAGTCGAGCTCAACCCGAGATCGGAAGAGCACACGTCTGAA1289920.10409764305979251Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGTCTACCTGCCGGACACTGAACCCTGGGTGGTAGATCGGAAGAGCA1273570.10277818412898468No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGTAGATCGGAAGAGCACACG1268380.10235934670691174No Hit
CTGCCGCTTCACCTATGGATTTTGTGCTCGCTGCAGATCGGAAGAGCACA1241010.10015056438665428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)