FastQCFastQC Report
Sat 23 Sept 2023
SRR18402615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18402615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145443324
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACAC28545051.962623599004104No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCAGATCGGAAGAGCACA15013291.032243322491722No Hit
CGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC10761030.7398778922296908TruSeq Adapter, Index 12 (100% over 42bp)
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACACG8684110.5970786256232703No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACA8076950.5553331550645804No Hit
CTTTGAGGCCGCGTGTGGCCCCTGGACTGTGTTAGATCGGAAGAGCACAC7422560.510340371483809No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGAGATCGGAAGAGCACACG7046260.48446775047577983No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC6706810.46112876243119966TruSeq Adapter, Index 12 (100% over 50bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT5248520.36086358972378824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT5053710.3474693688931367TruSeq Adapter, Index 12 (100% over 43bp)
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCAC4921780.338398481596859No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGCTAGATCGGAAGAGCAC4866930.3346272531560128No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCCCAGATCGGAAGAGCACAC4758940.3272023678446733No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGTAGATCGGAAGAGCACAC4473140.30755210187577947No Hit
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACAC4365430.30014646804964384No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGAGATCGGAAGAGCACACGTCT4340140.29840764640390094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC3978320.2735306022021334No Hit
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACG3601310.24760916492805127No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCGTC3533790.24296680678172622TruSeq Adapter, Index 12 (98% over 50bp)
GTTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACAC3502930.24084501809103318No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA3461300.23798273477303092No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3270330.22485253431089075No Hit
TGCCGCTTCACCTATGGATTTTGTGCTCGCTGCAGATCGGAAGAGCACAC3142290.21604910514833944No Hit
CAGCTCTGGTCAAGAGGATATCATATCCATCAGATCGGAAGAGCACACGT3017240.20745125434564463No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGT2961880.20364496069960558No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACGT2904660.19971078218756882No Hit
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2872410.19749342362389904Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCAGATCGGAAGAGCACACG2867810.19717714922411977No Hit
CCTATGGATTTTGTGCTCGCTGCTTGCCTTAGATCGGAAGAGCACACGTC2669400.1835354092979888No Hit
GGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2494680.17152248253072103Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGTAGATCGGAAGAGCACA2467580.16965921378419543No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAAGATCGGAAGAGCACACG2397190.16481952791452978No Hit
TTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACACGT2132080.14659180919159961No Hit
TTTGAGGCCGCGTGTGGCCCCTGGACTGTGTTAGATCGGAAGAGCACACG2020860.13894484424737158No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCGAGATCGGAAGAGCACA2006150.13793345372112095No Hit
ATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCCAGATCGGAAGAGCACAC1990220.13683818172362452No Hit
ACCTATGGATTTTGTGCTCGCTGCTTGCCTTCTTAGATCGGAAGAGCACA1885070.1296085614764965No Hit
TGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1868180.12844728438687222Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1832620.12600234576596997Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTGCTCGCTGCTTGCCTTCTTGCCGCTTCTGGTAGATCGGAAGAGCACA1763290.12123554051886218No Hit
TACCGTCTACCTGCCGGACACTGAACCCTGGGTGGTAGATCGGAAGAGCA1598990.10993904402239872No Hit
GAGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACA1597220.109817347133788No Hit
TATGGATTTTGTGCTCGCTGCTTGCCTTCTTGCTAGATCGGAAGAGCACA1585620.10901978560390987No Hit
TGTGCTGACTGCCTTGCTTTGTTTGCAATCTGGGAGATCGGAAGAGCACA1567930.10780350427084574No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCG1556200.10699700455140863TruSeq Adapter, Index 12 (97% over 42bp)
TGGATTTTGTGCTCGCTGCTTGCCTTCTTGCAGATCGGAAGAGCACACGT1546940.10636033043359212No Hit
AGAGTTCGATCGTCGAGGAGTACATTGGCCTAGAAGATCGGAAGAGCACA1535300.10556001869154201No Hit
CTACATCACGTACGTGTTTTGAACCGAGTTGAGCAGATCGGAAGAGCACA1507630.10365755942156549No Hit
CGGCTAGGTTCTCCGTCCTACTTTTCCCACATTGGAGATCGGAAGAGCAC1493540.10268879718398076No Hit
AGTTCGATCGTCGAGGAGTACATTGGCCTAGATAGATCGGAAGAGCACAC1474480.10137832108402584No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1458970.10031192631433533TruSeq Adapter, Index 12 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)