FastQCFastQC Report
Fri 30 Jun 2023
SRR1840399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51640489
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA23552494.560857276157861No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAATA21826694.2266621448917725No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19452703.76694728820248Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC14874602.880414242398053No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT11173542.163716923749502No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG10089031.9537053570503564No Hit
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8031051.5551847311128288No Hit
TAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT6232191.2068417864904417No Hit
TGTGGGTTGCACCGCCGACCGACCCTGCTGTAGGCACCATCAATAGATCG5271161.0207416897233486No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3976850.7701030871338186No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3565780.6905008200057904No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3416540.6616010162103616No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA3337050.6462080558532279No Hit
TTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCA3164190.612734321706365No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3116660.6035303035182336No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG3051610.5909335986342035No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGTCCTGTAGGCACCATCAATAGATC2967390.5746246903277774No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2930430.5674675156542378No Hit
TGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAA2851160.5521171575273038No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG2845550.551030800657213No Hit
GCCCCACGGTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2829750.5479711859428752No Hit
TTTCCGTCCAAGGCTAAATACTGGCGCTGTAGGCACCATCAATAGATCGG2699060.5226635247392797No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAA2627270.5087616424391334No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2574420.4985274248661743No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATA2534900.49087451515031166No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2396340.46404285598457445No Hit
ACCCAGGGCGCGAGCCCGGGCGGAGCGGTCGTCGGCTGTAGGCACCATCA2292330.4439016834251899No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATCA2286930.44285599232028966No Hit
ACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT2275170.4405787094696179No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2273660.4402863032532477No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC2120060.4105422007138623No Hit
AACCCAGGGCGCGAGCCCGGGCGGAGCGGTCGTCGGCTGTAGGCACCATC2021410.3914389733993417No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1954670.3785150059287781No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1887740.365554245623042No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGA1883380.36470994687908553No Hit
AATCCGGGCTAGAAGCGACGCGTGCGCCCGCCGCTGTAGGCACCATCAAT1853320.35888893306180736No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1760410.3408972366624956No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCCTGTAGGCACCATCAA1728710.3347586425837292No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC1698080.32882725026093385No Hit
GTCCGGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1618830.3134807650640179No Hit
ACGCGCGCTACACTGATGTATTCAACGCTGTAGGCACCATCAATAGATCG1579910.30594404324869967No Hit
TTCCGTCCAAGGCTAAATACTGGCGCTGTAGGCACCATCAATAGATCGGA1566500.3033472436715307No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTTCTGTAGGCACCATCA1544590.29910444883664833No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA1535020.2972512518229639No Hit
TAACCCAGGGCGCGAGCCCGGGCGGAGCGGTCGTCGGCTGTAGGCACCAT1524130.2951424414280817No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1497140.2899159223685895No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1485590.28767930528310837Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCCAGTGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1452620.28129478014818954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA1428900.27670148514666465No Hit
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG1374680.26620197186746236No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGCTGTAGGCACCATCAATAGATC1340210.2595269769811824No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA1334240.2583709073707648No Hit
GCCCCACGGTGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1270570.24604143465798708No Hit
TCCGTCCAAGGCTAAATACTGGCGCTGTAGGCACCATCAATAGATCGGAA1238170.23976728802858546No Hit
ATCCGGGCTAGAAGCGACGCGTGCGCCCGCCGCTGTAGGCACCATCAATA1232190.23860928195315886No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1188750.23019727795373898No Hit
TGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGCA1182210.22893082983780422No Hit
TGCCCCTCTTTTTGGACCCAAGGTCGGCCTGTAGGCACCATCAATAGATC1181400.22877397617206918No Hit
TTGCCGGTCGGGGGACGGATTGGGACTGTAGGCACCATCAATAGATCGGA1178040.2281233239290201No Hit
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1157650.2241748717755171No Hit
TAGGCCGAGGGCACGCCTGCCTGGGTGTCCTGTAGGCACCATCAATAGAT1038330.20106897128723936No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1026300.19873940388132263No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1011880.19594702133823713No Hit
TTTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGG1005800.19476965061271978No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCTGTAGGCACCATC1003580.19433975538070525No Hit
GGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAATA997880.19323597032553275No Hit
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC990010.19171197236339105No Hit
AATTCCGTCCAAGGCTAAATACTGGCGCTGTAGGCACCATCAATAGATCG982620.19028092472168495No Hit
CAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT926760.17946383118099443No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG926080.17933215156037738No Hit
GTCTAGTGGTCAGGACATTGGCTGTAGGCACCATCAATAGATCGGAAGAG914650.17711877205500512No Hit
CCGTCCAAGGCTAAATACTGGCGCTGTAGGCACCATCAATAGATCGGAAG893360.1729960380506854No Hit
TCCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC878640.17014556155732763No Hit
TCCGGGCTAGAAGCGACGCGTGCGCCCGCCGCTGTAGGCACCATCAATAG864770.1674596845897412No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC860440.1666211952408119No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATC843930.16342409151082982No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGCTGTAGGCACCATCAATAGATCGG838580.1623880827309749No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCTGTAGGCACCATC837620.16220218209010376No Hit
TGGGCCGCACGCGCGCTACACTGATGTATTCAACGCTGTAGGCACCATCA805830.15604615982625572No Hit
GCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT804960.15587768737046623Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC802750.15544972860346074Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC800520.1550178969064371No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT797990.15452797125914125No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAG779610.1509687485724622No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC772030.14950090809558367No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT770900.14928208754955824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTACCTGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT761960.14755088783144557No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA756700.14653230723667238No Hit
ACCGGTCGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAGATC736840.14268648772865028No Hit
ACGTCATCATCGCGATTGTGGATGGCAGCGCGCGCTGTAGGCACCATCAA736190.14256061750306045No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCTGTAGGCA716190.1386876874849113No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTGTAGGCACCATCAAT701430.13582946513151725No Hit
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC678550.13139883319075465No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAAT667020.12916608903529167No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT660750.12795192547460194No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGACTGTAGGCACCATCAATAGAT639370.1238117632852005No Hit
AGGGCGCGAGCCCGGGCGGAGCGGTCGTCGGCTGTAGGCACCATCAATAG619540.11997175317220563No Hit
TCGGCCGGTCGATCCGGGCGGAAGACATTGTCAGGCTGTAGGCACCATCA611810.11847486572019099No Hit
CTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCA610810.11828121921928353No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC604680.11709416616872083No Hit
AGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACC597750.11575219591743215No Hit
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC596890.11558565992665173No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGACGCCCTGTAGGCACCATCAA596300.11547140849111634No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCCTGTAGGCA594750.11517125641470978No Hit
AGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGATCGGA590900.11442571738621607No Hit
GTCTAGTGGCATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG588730.11400550447924689No Hit
TGCACCGGTCGGCTCGTCCCTTCTGCCGGCGCTGTAGGCACCATCAATAG588050.11387382485862983No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT582610.11282038789369325No Hit
CGCAGGTTCGTGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA559400.1083258526076312No Hit
TCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTGTAGGCACCATCAAT549260.10636227708842957No Hit
TGGTGGGATCCCCCTGTGGGTTGCACCGCTGTAGGCACCATCAATAGATC548390.1061938046326401No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT540770.1047182182957253No Hit
TTGACCGACGCGGATTGCGATGGTGGCCCCTGTAGGCACCATCAATAGAT539330.10443936733441853No Hit
TTCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG533780.10336462925438217No Hit
ATTCCGTCCAAGGCTAAATACTGGCGCTGTAGGCACCATCAATAGATCGG529970.10262683608592475No Hit
CGCAGGTTCGGGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA529420.10252033051042564No Hit
GTGTAGTGGTATCATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG525750.10180964785209529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)