FastQCFastQC Report
Sat 1 Jul 2023
SRR1840401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77525914
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23749003.0633627873126397No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAATA23225712.9958640668202894No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT16998002.192557188039086No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT14631801.8873431147164548No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC12080401.5582402549939625No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC11445991.4764082626616952No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10922421.4088734252136648No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA10875171.402778688942642No Hit
TAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT10832381.3972592441799525No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9017601.1631723555042512No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8093021.043911588065895No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC7785901.0042964472498834No Hit
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7239820.9338580645434249No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7184330.9267004578623864No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA7121690.9186205789202305No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6992400.9019435746349278No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6954590.8970664957268353No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6767780.8729700368318135No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG6763730.8724476308657257No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG6714430.8660884668834733No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5906200.7618355844214878No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5887130.7593757617614156No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAA5794980.7474894136688283No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5596310.7218631437224977No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5537870.7143250191155437No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5420490.6991842753379213No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5108110.6588906517116329No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5022580.6478582116426257No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTTCTGTAGGCACCATCA5020840.6476337705608992No Hit
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4486300.5786839223849718No Hit
TCCCCAGTGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4459110.5751767080101758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4405100.5682100052377326No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA4259630.5494459568706278No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4221340.5445069631813693No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG4191390.540643738814869No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCCTGTAGGCACCATCAA4065460.5244001379977281No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA3557160.4588349645255392No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3466690.44716531816703253No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3459900.44628948199178925No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA3381290.4361496466845912No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA3352310.4324115417716971No Hit
GCCCCACGGTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3298880.4255196526931627No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA3269090.4216770665870511No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA3193050.41186873333734575No Hit
ACCCGGGCTCAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3139570.40497039480243985No Hit
CTCCCGGCAAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3097850.3995889684060997No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCTGTAGGCACCATC2781720.35881163555195233No Hit
TCCACGTTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2720770.3509497482351514No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT2616900.3375516475690954No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCTGTAGGCACCATC2613750.33714533181769385No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATA2600270.3354065583799502No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATCA2510150.3237820582160437No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2464230.3178588774845015No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2287820.2951039055147418No Hit
TCCCCGGCAACGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2259270.2914212659266423No Hit
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2208710.28489957564382923No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC2089740.26955373915359454No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATC1977230.25504117242655144No Hit
ATCCCACAGCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1968380.25389961864880434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1932550.24927793821302124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC1922510.24798288737363353No Hit
CCCCTACCTGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1918430.247456611733723No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1837350.2369981732817752No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC1778440.2293994237849295No Hit
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1773730.22879188499473868No Hit
GTCCCACTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1771940.22856099445664066No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG1706600.22013284487042614No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC1698600.2191009318509937No Hit
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1673910.21591619029477033No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1622370.20926809066707683No Hit
TCCTCGCCGGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1538180.19840849602882463No Hit
TCCCCAGTGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1521840.19630081368663385No Hit
ACCACGTGGTCGTGGGTTCGTTCCCCACAGACGGCGCTGTAGGCACCATC1517230.19570617380918592No Hit
GCCCCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1486640.1917603964011311No Hit
CCCCTACCTGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1415360.18256605139798804No Hit
ATCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1406840.18146706403229249No Hit
TCCCCACAGACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1356430.1749647221185938No Hit
CAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT1353680.1746100020181639No Hit
GCCCCACGGTGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1328140.17131561970362583No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAAT1247080.16085976103422656No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGACGCCCTGTAGGCACCATCAA1225240.158042638491176No Hit
TTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCA1222050.15763116317467732No Hit
ACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1188480.15330099816688392No Hit
AAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCAT1149060.14821624676363054No Hit
TTCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1136590.14660775234459023No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTGTAGGCACCATCAAT1132270.14605051931409668No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1110700.14326822383545199No Hit
CGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCAA1091620.1408071112841056No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCGGCTGTAGGCACCATCAATA1088800.14044336194475568No Hit
TGGGCCGCACGCGCGCTACACTGATGTATTCAACGCTGTAGGCACCATCA1087230.14024084901469203No Hit
GTGTAGTGGTATCATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG1058860.13658142746952975No Hit
ACCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1053700.1359158435719958No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGACGCCCTGTAGGCACCATCAA1035310.13354373351857549No Hit
CGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCAA997010.12860344993804265No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC993620.12816617679605816No Hit
TCCTGCGTGGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT974470.12569603500579174No Hit
ACGTCATCATCGCGATTGTGGATGGCAGCGCGCGCTGTAGGCACCATCAA957720.12353546712135506No Hit
TCCCCGGCAACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT956210.12334069353893719No Hit
GCCCTCCTTCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT954530.12312399180485636No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAAT954490.12311883223975921No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT948180.12230491084568187No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT913280.11780319029840783No Hit
ACCCCACAGCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT912490.11770128888773888No Hit
ACCCCACAGCCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT900410.1161431002283959No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCCTGTAGGCACCATCAAT896830.11568131915219985No Hit
GCCCTCCTTCTAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC882310.11380839702192999No Hit
ACCCGGGCTCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT871160.11237016825109603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT870940.11234179064306161No Hit
TGCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT860660.11101578241309092No Hit
GCCTCTCTCACCCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC853380.1100767415654074No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGA843900.10885392463737996No Hit
AGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATC834130.1075937008623981No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCCTGTAGGCACCAT824300.10632573773977046No Hit
TTCACGTCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG814620.10507712298625721Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCCGCATCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC812310.1047791581018961No Hit
ACGGTACAGACCCGGGTTCGATTCCCGGCTGGTGCCTGTAGGCACCATCA809530.10442056832764332No Hit
GCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAAT803730.1036724313885548No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG791690.10211940229430896No Hit
TCCTGCGTGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT791570.10210392359901749No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAG781600.1008179019985498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)