FastQCFastQC Report
Fri 30 Jun 2023
SRR1840403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50150107
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA23407924.667571297504908No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA18484133.685760829981878No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA11274762.248202581103167No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT10922662.1779933590171603No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCCTGTAGGCACCATCAA8933151.7812823410326921No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA7979391.591101291169728No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA7495261.4945651063117373No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6788341.3536042904155716No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG6053151.20700639781287No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA5800471.1566216598500978No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC5567181.1101033144356003No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA5275311.0519040368149164No Hit
ACGTCATCATCGCGATTGTGGATGGCAGCGCGCGCTGTAGGCACCATCAA5089751.0149031187510726No Hit
TAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT4775220.9521854061049162No Hit
TTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCA4584240.9141037326201517No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4415380.8804328174215063No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCCTGTAGGCACCATCAAT3861650.7700182972690367No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCCTGTAGGCACCATCAAT3816370.7609894032728585No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG3277280.6534941191650897No Hit
ATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATCAAT3169570.6320165976914067No Hit
ACGGTACAGACCCGGGTTCGATTCCCGGCTGGTGCCTGTAGGCACCATCA3158790.6298670509317159No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTGTAGGCACCATCAAT2863000.5708861199438717No Hit
TTTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATCAAT2477980.4941126047846718No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC2387740.4761186252304507No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCGGCTGTAGGCACCATCAATA2219180.4425075304425572No Hit
TTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATCAATA2173190.43333706147426565No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA2049630.40869902829918187No Hit
ACGAATACGAACCGTGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA2003510.3995026371528978No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC1965590.3919413372338368No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAATA1794670.35785965521469376No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCTGTAGGCACCATC1594380.31792155498292357No Hit
TCCCCACAGACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1436270.28639420450289366No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCTGTAGGCACCATC1415310.2822147518050161No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA1361930.271570706718532No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGACGCCCTGTAGGCACCATCAA1343400.2678757993477462No Hit
ATGAAAATCGGTCGCCCTCGGCGTCCGAATTGTAGTCTGTAGGCACCATC1334400.26608118702518424No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1320070.26322376540492726No Hit
TCCCCGGCAACGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1263250.2518937796084862No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAAT1251280.2495069452194788No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGACGCCCTGTAGGCACCATCAA1225250.2443165275798913No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1197910.23886489414668646No Hit
AAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCAT1102390.21981807536322903No Hit
TAAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCA1093840.21811319365679518No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCTGTAGGCACCATCAA1093750.21809524753356957No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCGGCCTGTAGGCACCATCAAT1031220.20562667991914754No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAATAG1024330.20425280448554176No Hit
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1007190.20083506501790716No Hit
ACGGTACAGACCCGGGTTCGGTTCCCGGCTGGTGCCTGTAGGCACCATCA967080.19283707610035608No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGATGCGCCTGTAGGCACCATCA964840.1923904170334073No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT964410.1923046744446627No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC939820.18740139477668513No Hit
TTGCGATGGTCCCTGCGGATGTTGACGCAACTGTAGGCACCATCAATAGA934830.18640638194450912No Hit
ACGCGGGTGACCCGGGTTCGATCCCCGGCAACGGCGCCTGTAGGCACCAT919330.1833156607223191No Hit
TCGGCCGGTCGATCCGGGCGGAAGACATTGTCAGGCTGTAGGCACCATCA917550.18296072628519017No Hit
TTTGCGATGGTCCCTGCGGATGTTGACGCAACTGTAGGCACCATCAATAG901540.17976831036472166No Hit
ACGTCATCATCGCGATTGTGGATGGCAGCGCGCCTGTAGGCACCATCAAT893680.17820101560301757No Hit
TCCGTCCAAGGCTAAATACTGGCGAGAGACCGCTGTAGGCACCATCAATA882710.17601358258318373No Hit
ACCTTGTGGTCGTGGGTTCGAGCCCCACGGTGGGCGCTGTAGGCACCATC850610.16961279863271278No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT842070.16790991093997068No Hit
ACGGTACTGACCGGGGTTCGATTCCCCGGTTGCGCCTGTAGGCACCATCA815940.16269955316346585No Hit
TTGGGCCGCACGCGCGCTACACTGATGTATTCAACGCTGTAGGCACCATC808640.16124392316849892No Hit
GTCTAGTGGTAGAATAGTACCCTCTGTAGGCACCATCAATAGATCGGAAG788100.15714821904567422No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG773580.1542529111652743No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG757750.151096387491257No Hit
TTTCCGTCCAAGGCTAAATACTGGCGAGAGACCGCTGTAGGCACCATCAA725870.14473947184200425No Hit
TCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCCTGTAGGCACCATCAA715350.1426417694382985No Hit
TTGGCGTGCCTCGGCACCTGCGCGCTCCTGGCGTCGGCCTGTAGGCACCA697890.13916022153252836No Hit
ACCACGTGGTCGTGGGTTCGATCCCCACAGACGGCGCTGTAGGCACCATC690830.13775244786616306No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATC659300.13146532269612107No Hit
ACCACGTGGTCGTGGGTTCGTTCCCCACAGACGGCGCTGTAGGCACCATC656650.13093690906781116No Hit
TTGCGATGGTCCCTGCGGATGTTGACGCAATCTGTAGGCACCATCAATAG647560.1291243506220236No Hit
ATCCGGGCTAGAAGCGACGCGTGCGCCCGCCGCTGTAGGCACCATCAATA647390.1290904523892641No Hit
TTGGCGTGCCTCGGCACCTGCGCGCTCCTGGCGTCGGCCCTGTAGGCACC640400.127696636818741No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA638180.12725396577917572No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT634360.12649225254893276No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA632960.1262130906320898No Hit
AGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATC629820.12558697033288482No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAAT618170.12326394438201298No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCCTGTAGGCACCAT617580.12314629757420059No Hit
TCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGATCG597600.11916225821811308No Hit
TTTGCGATGGTCCCTGCGGATGTTGACGCAATCTGTAGGCACCATCAATA581350.1159219859690429No Hit
TGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAA576300.11491500905471647No Hit
TCGTAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAATAGAT574420.1145401344806702No Hit
TTGCGAGAGGTACGGGGATCGATACCCCGCATCTCCCTGTAGGCACCATC568340.11332777415609503No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT558960.11145738931324711No Hit
ATGCTCGTGGAGACGTCATCATCGCGATTGTGGCTGTAGGCACCATCAAT554810.1106298736311769No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCCTGTAGGCACCAT554140.11049627471383063No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT546560.1089848123354951No Hit
TCCGTCCAAGGCTAAATACTGGCGAGAGACCGACTGTAGGCACCATCAAT544390.10855211136438853No Hit
ATTGCGATGGTCCCTGCGGATGTTGACGCAATCTGTAGGCACCATCAATA539210.10751921227206954No Hit
ACCCAGGGCGCGAGCCCGGGCGGAGCGGTCGTCGGCTGTAGGCACCATCA535470.10677345115136046No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTGTAGGCACCATCAATA529620.10560695314169519No Hit
TCGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA526840.10505261733539273No Hit
TCCCCACAGACGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA525920.10486916807575307No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAAT523030.1042928981188415No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG516780.10304663956150681No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCCTGTAGGCACCATCAA509400.10157505745700603No Hit
TCCCCGGCAACGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC508850.10146538670396058No Hit
ATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATCAATAGATCG508450.10138562615629115No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT507600.10121613499249364No Hit
TCATAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAATAGAT507540.10120417091034323No Hit
TTGTAGTATAGTGGTAAGTATTCCCGCCCTGTAGGCACCATCAATAGATC505010.10069968544633415No Hit
TACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATC502490.1001971939960168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)