FastQCFastQC Report
Fri 30 Jun 2023
SRR1840405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58241888
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23716384.072048625896193No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11436521.9636245308531208No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8922871.5320365301344627No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8430891.4475646805955191No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7655221.3143839018405445No Hit
ACCCGGGCTCAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7593731.303826208381157No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7419771.2739576711524188No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7228961.2411960271617568No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC7128631.223969593842837No Hit
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7054451.2112330561811457No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6666281.1445851480638816No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6496511.1154360243266839No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6414661.1013825650706928No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6414611.1013739801841589No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6378651.0951997297889795No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC6019691.0335671123848182No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5776380.9917913375335634No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5284930.9074104877918793No Hit
CTCCCGGCAAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4528210.7774833810332522No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG3952350.6786095258450413No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3152100.5412084168700025No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3115790.5349740722690858No Hit
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3051570.5239476440049471No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3041440.5222083459931793No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2926020.502390993918329No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2726600.46815103246652995No Hit
TCCACGTTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2707870.4649351339709317No Hit
GTCCGGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2633800.4522174830596151No Hit
TGTGGGTTGCACCGCCGACCGACCCTGCTGTAGGCACCATCAATAGATCG2474200.4248145252434124No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2163830.3715247005728935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATC2080960.35729610963161085No Hit
TCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAATAGATCGGAAGA2044330.3510068217568771No Hit
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2042350.35066686025013477No Hit
ACAGCGAAAGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAA1861500.3196153256570254No Hit
CCCCTACCTGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1756270.30154757345778355No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1726150.29637603780976335No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA1714050.29429849526856No Hit
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1690250.29021208927842446No Hit
GCCTCCCACGCCCGGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAG1662850.28550757145784833No Hit
ACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT1662430.2854354584109636No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA1611260.2766496855321723No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA1605990.27574483849149944No Hit
TGCCTCCCACGCCCGGTCGTACTCCTGTAGGCACCATCAATAGATCGGAA1529480.2626082451173286No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAATA1521400.2612209274534507No Hit
ATCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1507140.25877251781398297No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1464050.2513740625990696No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1435110.24640513027324937No Hit
TTGCCGGTCGGGGGACGGATTGGGACTGTAGGCACCATCAATAGATCGGA1356750.23295089609732433No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1320020.2266444384495228No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1319640.22657919331186518No Hit
TCCTGGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1270480.2181385328717366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1266620.21747577963131967No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1225380.2103949652181605No Hit
CTCTCAACGAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1208610.2075155942746911No Hit
TTGCCTCCCACGCCCGGTCGTACTCCTGTAGGCACCATCAATAGATCGGA1166150.20022530863010485No Hit
GCCTCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1158650.19893757565002013No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC1149460.19735967350508968No Hit
AAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCAT1138210.1954280740349626No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATA1108450.19031834956998647No Hit
TCCTGGGCAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1104750.18968306796647802No Hit
ACCCCACAGCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1097110.18837129730409838No Hit
ACCCCACAGCCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1074860.18455102279651373No Hit
TTCTCGCAACACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1073160.1842591366543612No Hit
ACCCGGGCTCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1071880.18403936355909342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCAGTGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1024460.17589745717034447No Hit
GCCTCTCTCACCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1012030.1737632543780174No Hit
TACCCCGCATCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1001770.17200163566126153No Hit
TTACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG996300.17106244907445306No Hit
ACCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT990440.1700563003726802No Hit
ATCTCTCAGGCGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC975930.16756496630054302No Hit
ACCCTGCTGTCGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC961260.16504616059149732No Hit
TTCTCGCAACACCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC950860.1632605041924465No Hit
ATCCTGCCGTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT937890.16103358462555334No Hit
ATCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC937520.1609700564652025No Hit
TGGGTTGCACCGCCGACCGACCCTGCTGTAGGCACCATCAATAGATCGGA922420.15837742073196528No Hit
TAAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCA913340.1568184053374094No Hit
ATCCTGCCGTTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT900750.15465673090817386No Hit
ACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC898150.15421031680841116No Hit
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG881000.15126570072728412No Hit
TCCCCGGCAACGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA877560.15067506053375193No Hit
TAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT868310.1490868565249808No Hit
TCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA834520.14328519020537248No Hit
ATCTCTCAGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA833600.14312722829314875No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT829040.14234428664125723No Hit
ATCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG812320.13947350058432173No Hit
ATCTCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT809710.13902536950725225No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC798330.13707144933213705No Hit
GCCTCTCTCACCCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC796880.13682248762265398No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT795290.13654948823087604No Hit
TTGGGTTGCACCGCCGACCGACCCTGCTGTAGGCACCATCAATAGATCGG770550.13230168637390324No Hit
AGCTGGGTTACGGTGCCCAACTGCGCGCTACTGTAGGCACCATCAATAGA755290.12968157900375757No Hit
CTCTCAACGAGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC737800.12667858569420004No Hit
ATCCCAGCAGCCACACTGTAGGCACCATCAATAGATCGGAAGAGCACACG728920.12515390984577973No Hit
TGCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT725100.12449802451458991No Hit
TAGCCGCGCCATGGAATCGAGAGCTCCCTGTAGGCACCATCAATAGATCG706910.1213748427935578No Hit
TCTTTGAACGCAAGTTGCGCCCGAAGCCCTGTAGGCACCATCAATAGATC703640.12081339121424087No Hit
GCCCCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT702500.12061765580126799No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA698800.11998237419775953No Hit
TTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCA673020.11555600670088167No Hit
TAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCAT672780.11551479924551897No Hit
GCCCTCCTTCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT659190.11318142708560547No Hit
ACCCTGCTGTCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA649840.11157605330376653No Hit
ACGCGCGCTACACTGATGTATTCAACGCTGTAGGCACCATCAATAGATCG640630.1099947172042225No Hit
GTCTAGTGGTAGAATAGTACCCTCTGTAGGCACCATCAATAGATCGGAAG633360.10874647470219372No Hit
TCCCCGGCAACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT623810.10710676137421918No Hit
TTCACGTCGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT623740.10709474253307173No Hit
GTTACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC620680.10656934747719717No Hit
TCCGGGCTAGAAGCGACGCGTGCGCCCGCCGCTGTAGGCACCATCAATAG620370.106516121180687No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA606060.10405912665468536No Hit
TTCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG593160.10184422592893966No Hit
TACCCCGCATCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC593130.10183907499701933No Hit
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC587650.1008981714329041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)