FastQCFastQC Report
Fri 30 Jun 2023
SRR1840407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86277212
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC46761045.419859881425005No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG27866573.229887632437636No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA22710112.632225760841693No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG14328281.6607258936461693No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATCA8976991.0404821611528199No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA7633190.8847284031384788No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA6803390.788550051895511No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG6693240.7757830654054978No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATC6520840.7558009639903525No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA6507230.7542234906709782No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA6324760.7330742212671406No Hit
CGCAGGTTCGTGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA5744480.6658166005642371No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA5458700.6326931380211962No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAAT5115350.5928969981088401No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC5042450.5844474900278419No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT4549120.5272678491279946No Hit
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4427790.5132050395879737No Hit
CGCAGGTTCGGGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA4403860.5104314219147462No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC4321840.5009248560326682No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG4039410.4681896767827871No Hit
TTTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGG3884230.4502034673999434No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCTGTAGGCACCATC3574530.4143075462382813No Hit
TGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGCA3437450.3984192256931065No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC3286330.3809035924804802No Hit
CGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCAA3046820.35314307560146935No Hit
GCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAAT2987680.3462884266589421No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAA2969420.34417199294756995No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG2944350.34126624304920744No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAG2917000.3380962287005751No Hit
GCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTCTGTAGGCACCATCAATA2892370.3352414772048962No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2774340.3215611556850029Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG2729760.31639409024946236No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCGGCTGTAGGCACCATCAATA2705730.31360888203017034No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGACGCCCTGTAGGCACCATCAA2702540.31323914361071376No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGACGCCCTGTAGGCACCATCAA2681110.310755289589098No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2535360.2938620687001337No Hit
TGCGAGAGGTACGGGGATCGTTACCCCGCATCTCCCTGTAGGCACCATCA2530550.29330456343443273No Hit
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA2450440.2840193769821862No Hit
CGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCAA2265700.2626070021826853No Hit
AAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCATCAATAGA2256480.2615383538355412No Hit
CGGAGGTTCAGGCCCTCCTTCTAGCGCCTGTAGGCACCATCAATAGATCG2256360.26152444518026385No Hit
ACCCGAAAGACCCGGGTTCAGGTCCCGGCAACGGCTGTAGGCACCATCAA2249470.2607258565564219No Hit
ACCACGTGGTCGTGGGTTCGTTCCCCACAGACGGCGCTGTAGGCACCATC2157190.2500301006481294No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTTCTGTAGGCACCATCA2119160.24562221597981165No Hit
GTGTAGTGGTATCATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG2081670.24127692026024206No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG2069850.23990691771542177No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2066350.23950124860316535No Hit
TGTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATA2055700.23826685544729934No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2040420.2364958200086484No Hit
GTCTAGTGGTCAGGACATTGGCTGTAGGCACCATCAATAGATCGGAAGAG1957820.22692202895939662No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1909560.22132843142868364No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1884740.2184516578954823No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAAT1854880.2149907208406317No Hit
TGCGAGAGGTCCCGAGTTCGTTTCTCGGAATGCCCCTGTAGGCACCATCA1818170.21073583138036495No Hit
TGCGAGAGGTACGGGGATCGGTACCCCGCATCTCCCTGTAGGCACCATCA1809550.20973672630960769No Hit
TTCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAAT1761190.20413153823283023No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1675280.1941741001088445No Hit
CTCTGGTTCAAGTCCAGGATGGCCCCTGTAGGCACCATCAATAGATCGGA1652020.1914781390942489No Hit
TGCGAGAGGTCCCGAGTTCATTTCTCGGAATGCCCCTGTAGGCACCATCA1608350.18641654762789506No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCCTGTAGGCACCATCA1598040.18522156232864828No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCTGTAGGCACCATCAA1568960.18185103153310053No Hit
CGGAGGTTCAGGCCCTCCTTCTAGCCTGTAGGCACCATCAATAGATCGGA1555190.18025501334002308No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCTGTAGGCACCATC1544620.17902989262100866No Hit
CCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCATCAATAGATCG1464280.1697180479128139No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCAACTCCCTGTAGGCACCATCA1450530.1681243478289493No Hit
ACCCGAGAGACCCGGGTTCAGGTCCCGGCAACGGCTGTAGGCACCATCAA1433300.16612729674204124No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCGTCTCCCTGTAGGCACCATCA1433210.16611686525058322No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1383190.16031927410913557No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCATCTCCCTGTAGGCACCATCA1349290.15639007899328042No Hit
GTCTAGTGGTATGATTCTCGCTTGCTGTAGGCACCATCAATAGATCGGAA1327140.15382277304000042No Hit
ACCCAGGAGACCCGGGTTCGTTTCCCGGCAATGGCTGTAGGCACCATCAA1321500.15316906624196433No Hit
GCCGTCTTAGCTCAGTGGTAGAGCGTGGCTGTAGGCACCATCAATAGATC1312260.15209809978560734No Hit
AGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCATCAATAGATCGGAAG1307830.15158463859495136No Hit
ACCCAGGAGACCCGGGTTCGTTTCCCGGCAACGGCTGTAGGCACCATCAA1287640.14924450734453495No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG1287470.14922480341622535No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA1278350.14816774561514576No Hit
TGCGAGAGGCACGGGGTTCGTTCCCCCGCATCTCCCTGTAGGCACCATCA1230410.14261123783183907No Hit
CTCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAAT1229330.1424860599343428No Hit
GCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCATCAAT1215630.14089815512351048No Hit
ACCCGAAAGACCCGGGTTCATGTCCCGGCAACGGCTGTAGGCACCATCAA1201690.13928243300212342No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGATGCGCCTGTAGGCACCATCA1199340.1390100551696084No Hit
TTGCGAGAGGTACGGGGATCGATACCCCGCATCTCCCTGTAGGCACCATC1198540.13891733080109264No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC1182980.1371138418334612No Hit
TTCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1168110.1353903276336746No Hit
TAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCAC1164360.13495568215625697No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCAACTCCCTGTAGGCACCATC1156280.13401916603424782No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1147460.13299687987136163No Hit
GTCTAGTGGTATGATTCTTGCCTGTAGGCACCATCAATAGATCGGAAGAG1141990.13236287700163515No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1133970.1314333152072647No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCTGTAGGCACCA1132940.13131393258280066No Hit
ACCCGAGAGACCCGGGTTCATGTCCCGGCAACGGCTGTAGGCACCATCAA1132210.13122932159653003No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCGTCTCCCTGTAGGCACCATC1126930.13061734076432605No Hit
TTGCGAGAGGTACGGGGATCGTTACCCCGCATCTCCCTGTAGGCACCATC1122570.1301119929559152No Hit
TGCGAGAGGTACGGGGATCGATACCCCGCATCTCCCTGTAGGCACCATCA1087150.12600662153988007No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC1076290.12474788823727868No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1074660.12455896233642784No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCATCTCCCTGTAGGCACCATC1065620.12351117697219981No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1041210.12068192467786279No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCCTGTAGGCACCATCA1024420.11873587199363836No Hit
CAGCGGTTCGAGTCCGCTTATCTCCCTGTAGGCACCATCAATAGATCGGA1014160.1175466819674238No Hit
TTGCGAGAGGTACGGGGATCGGTACCCCGCATCTCCCTGTAGGCACCATC999650.11586489373346927No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT988270.11454588959133263No Hit
GAAACTCTGGTGGAGGCCCGCCTGTAGGCACCATCAATAGATCGGAAGAG985940.1142758298680305No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG984090.11406140476583781No Hit
TACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATC957980.11103511318840482No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATA950200.1101333687045891No Hit
CGGAGGTTCAGGCCCTCCTTCTAGCTGTAGGCACCATCAATAGATCGGAA949040.10999891837024127No Hit
CCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATAG933770.10822904198619678No Hit
TGCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAA928910.10766574144746356No Hit
CGCAGGTTCGAGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA917280.10631776094016576No Hit
AGCGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA903170.1046823348904691No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG895240.10376320458755667No Hit
TGCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTCTGTAGGCACCATCAAT880510.10205591715226033No Hit
TGCCCCTCTTTTTGGACCCAAGGTCGGCCTGTAGGCACCATCAATAGATC878090.10177542593750016No Hit
GTCTAGTGGCATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG863610.10009711486736499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)