FastQCFastQC Report
Mon 3 Jul 2023
SRR1840409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100907777
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT54536335.404571542587842No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC48837334.839798423069016No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT25906692.567363068557144No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT24137382.392023758485929No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23819332.360504879618942No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC20770182.0583329271043205No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC20304162.0121501636092924No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT17976271.781455357994855No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT15667451.552650396807374No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT15574351.543424150548872No Hit
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT14155051.4027709677917095No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT12452581.2340555277518401No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11296511.1194885405116No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11154881.105452952352721No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10022170.9932009502102104No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG9345730.9261654827655157No Hit
GTCCGGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8660630.8582718059481184No Hit
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC8568940.8491852912387515No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG8299460.8224797182877194No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG8010080.7938020475864808No Hit
ACCCGGGCTCAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7648560.7579752747897717No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG7168620.7104130338734942No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7159300.7094894182437494No Hit
AGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAATA7000980.6937998445848232No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA6890550.6828561885770212No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6850660.6789030740415578No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6703350.6643045956705597No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6268420.6212028632837685No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5859240.5806529659255104No Hit
CTCCCGGCAAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5705590.5654261910853511No Hit
TCCACGTTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5244330.5197151454441415No Hit
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC5045260.49998723091481845No Hit
ATCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5013600.496849712584591No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4644060.4602281546644319No Hit
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4281700.42431813754057823No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG4173800.41362520551810394No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3207560.3178704452085987No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3091700.3063886740860419No Hit
GCCCCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3090760.3062955197199518No Hit
TCCCCAGTGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2879210.2853308323301979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCAGTGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2861400.28356585439395815No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATCA2792400.2767279275213842No Hit
ACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2430930.24090610974414786No Hit
ACCCCACAGCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2367380.23460827999411782No Hit
CCCCTACCTGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2321260.23003777003233358No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA2316940.22960965634987676No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC2157950.21385368543001396No Hit
TGCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2059380.20408536004117897No Hit
ACCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2012010.19939097459257277No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA1964980.19473028327638217No Hit
TAGGCCGAGGGCACGCCTGCCTGGGTGTCACACCTGTAGGCACCATCAAT1937030.1919604273910424No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA1935240.19178303769391333No Hit
ATCTCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1928340.19109924500665593No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1897660.18805884505809695No Hit
TTCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1858710.18419888488872369No Hit
ACCCGGGCTCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1834010.18175110526912114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACGTCGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1828460.18120109810763146No Hit
ATCCTGCCGTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1823960.1807551463550723No Hit
TTGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATC1804900.1788662929320106No Hit
CTCTCAACGAGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1800860.17846592735860192No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA1784870.17688131213117497No Hit
GCCTCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1744870.17291729655287125No Hit
ATCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1691970.16767488595056454No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1679470.16643613108234462No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1636510.16217877835124642No Hit
TTCTCGCAACACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1618180.16036226821248872No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1607150.15926919091677147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1591120.15768061167376624No Hit
CGCAGGTTCGTGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA1530490.15167215506095233No Hit
ACCCCACAGCCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1529770.15160080278054286No Hit
TACCCCGCATCTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1468940.14557252608983745No Hit
TTCCCCGCAACTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1427300.14144598587282328No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA1416020.1403281334797416No Hit
GCCTCTCTCACCCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1413610.14008930154114882No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCTGTAGGCACCATC1406320.13936685970200297No Hit
TCCCCGGCAACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1403020.13903982841679288No Hit
TTCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAAT1400710.13881090651714584No Hit
GTCCCACTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1378660.13662574292960591No Hit
TTCTCGCAACACCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1375400.13630267565997417No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCTGTAGGCACCATC1331920.131993790726358No Hit
TGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGCA1288910.12773148297578688No Hit
CTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1273780.1262320940832935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT1259030.12477036333879399No Hit
ATCCTGCCGTTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1258870.12475450727648077No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC1243790.12326007340346028No Hit
TTCCCCGCGTCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1235000.12238898098012801No Hit
TCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1201880.11910677608129253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACCA1196460.11856965197043236No Hit
TTTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGG1192230.11815045732302674No Hit
CGCAGGTTCGGGACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGA1166380.11558871225554797No Hit
TCCTGGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1147080.11367607473901639Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1132460.11222722704514639No Hit
GTCCGGGCGAAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1102320.10924034130689451No Hit
CTCTCAACGAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1099700.10898069828651563No Hit
CCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1091470.10816510208127962No Hit
ACCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1089560.10797582033741562No Hit
TTCACGTCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1043180.10337954427437245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC1040500.10311395523062608No Hit
TCCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1037740.10284043815572312No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1035210.10258971417039542No Hit
TCCCCAGTGGAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1035180.10258674115871168No Hit
ATCCCACAGCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1033180.10238854037979649Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCGGTGGAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1032770.10234790922011888No Hit
ACCCTGCTGTCGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1029100.10198421079080952No Hit
TTCTCGCAACACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1019940.10107645122337797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)