FastQCFastQC Report
Fri 30 Jun 2023
SRR1840413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72276634
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC18117362.5066690294404137No Hit
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT17960262.4849330974654964No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11745561.6250839794227274No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11146131.5421484625307813No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC10029431.387644864590678No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC9713201.3438921353199706No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7948081.0996748963157303No Hit
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7412651.0255942466828214No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7215470.9983129540869322No Hit
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7120200.9851316540280499No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6227170.8615744335852719No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG6180510.8551186819242301No Hit
ACCCGGGCTCAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6166920.853238406204694No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6094300.8431908990117054No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5946910.8227984164287451No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5245920.7258113320551148No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5211210.7210089501400965No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5143130.7115895850932958No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5046600.6982339548352514No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4923960.6812658154501219No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4615060.6385272451951761No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4525660.6261581024927088No Hit
CTCCCGGCAAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4414240.610742332023929No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG4007340.5544447462785829No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4004540.554057345836No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3706690.5128476237562474No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3554690.49181731401603457No Hit
TCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAATAGATCGGAAGA3193490.4418426569228445No Hit
TCCACGTTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3174640.4392346218004563No Hit
CTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC2986910.41326080569828416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2946700.4076974586281924No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2935390.4061326375547594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2933180.4058268679197207No Hit
ACAGCGAAAGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAA2922420.4043381433617952No Hit
CCCCTACCTGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2689720.37214239943714034No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA2665290.3687623305756048No Hit
GTCCGGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2630200.3639073728862359No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA2611700.3613477628191706No Hit
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2598460.3595159121549573No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA2408680.33325846358589417No Hit
GCCTCCCACGCCCGGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAG2317040.32057940052936057No Hit
CAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC2303330.3186825219337137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCCACGGTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2281010.3155943869771246No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC2279900.3154408103731006No Hit
TTGCCGGTCGGGGGACGGATTGGGACTGTAGGCACCATCAATAGATCGGA2189170.3028876524604065No Hit
GTCTAGTGGTAGAATAGTACCCTCTGTAGGCACCATCAATAGATCGGAAG2075610.28717579736765275No Hit
TCCTGGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2071690.2866334367480367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATA2068550.2861989948231402No Hit
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG1984190.27452717291732204No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1872400.25906021024720105No Hit
TCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1870510.25879871494845763Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1840350.25462585875263644No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1681450.23264088363605864No Hit
ACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCAATAGAT1678020.23216631809389465No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1625240.22486381975120756No Hit
ACCCCACAGCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1616400.22364074121105307No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1606460.2222654696398839No Hit
AGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCATC1519770.21027127522291644No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT1495980.2069797550339713No Hit
ACCCCACAGCCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1482540.20512023290957354No Hit
TCCCCGGCAACGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1461460.2022036610061282No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1437200.1988471128857495No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG1395000.19300843478682198No Hit
TCCCCAGTGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1393530.19280504955446598No Hit
ATCCCAGCAGCCACACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1367870.18925480121279584No Hit
CTCTCAACGAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1315070.1819495357240903No Hit
ATCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1231230.17034965961475185No Hit
TCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1212220.16771948732421602No Hit
TCCTGGGCAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1203720.16654345026637518No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1198730.1658530473347721No Hit
ATCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1186120.16410836177014No Hit
ACCCGGGCTCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1185830.16406823815287247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCGCATCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1127570.15600754180113036No Hit
ATCTCTCAGGCGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1114550.15420612974312004No Hit
ATCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1106990.15316014854814627No Hit
ATCTCTCAGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1095670.1515939439017041No Hit
GTCTAGTGGTCAGGACATTGGCTGTAGGCACCATCAATAGATCGGAAGAG1082920.14982988831494282No Hit
CGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1074390.14864970053807433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCCTCCCACGCCCGGTCGTACTCCTGTAGGCACCATCAATAGATCGGAA1058120.14639862725206598No Hit
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1050330.1453208238778801No Hit
ATCCCACAGCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1044210.14447407719623467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTCCTTCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1032490.14285252962942355No Hit
TTCACGTCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1006970.13932165130988253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCGCAACACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1002660.13872533134290677No Hit
TTCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG984510.13621414633116424No Hit
AAGCATTGCGATGGTCCCTGCGGATGTTGACGCAATGCTGTAGGCACCAT974780.13486792979318876No Hit
TTGCCGGTCGGGGGACGGATTGGGCTGTAGGCACCATCAATAGATCGGAA961910.13308727133031678No Hit
TTCACGTCGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT955110.1321464416840441No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG948650.13125265352008508No Hit
CCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA933740.1291897461633313Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCTGCTGTCGACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC920860.1274077041274501No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA919740.12725274395041694Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAAT915210.12662598537723824No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA899190.12440950141646054No Hit
TTCTCGCAACACCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC896080.1239792102105917No Hit
ACCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT893120.12356967259986125No Hit
AGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAAT886850.12270217232307748No Hit
CTCTCAACGAGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC870030.12037500252156182No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA864370.11959190019834073No Hit
GCCTCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT859110.11886414079548863No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA842060.11650514881476079No Hit
TGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC840140.11623950279698969Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC825760.11424992480972482No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC822990.11386667508616961No Hit
GCCCTCCTTCTAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC818220.11320671076076952No Hit
GTGTAGTGGTATCATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG812570.11242499201055765No Hit
ACCTTGTGGTCGTGGGTTCGTGCCCCACGGTGGGCGCTGTAGGCACCATC786990.1088858122529613No Hit
ATCCTGCCGTTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT785300.10865198841440236No Hit
ATCTCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT784150.10849287751834155No Hit
GCCCCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT782070.10820509433242284No Hit
TCCGGTGCGCCCCCGGCGGCCCCTGTAGGCACCATCAATAGATCGGAAGA781290.10809717563770332No Hit
TTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCCTGTAGGCACCATCA779630.10786750251817205No Hit
TTCTCGGAATGCCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA770830.10664995827005448No Hit
ACCACGTGGTCGTGGGTTCGTTCCCCACAGACGGCGCTGTAGGCACCATC770660.10662643752889765No Hit
ACCTTGTGGTCGTGGGTTCGGGCCCCACGGTGGGCGCTGTAGGCACCATC765530.10591666457516546No Hit
ATCCCATTCTTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT765090.10585578736275959No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGCTGTAGGCACCATCAATAGATC763830.10568145716359731No Hit
TCCCCGGCAACGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC763210.10559567563702538No Hit
ATCCTGCCGTCCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG755190.1044860500836273No Hit
ACCCTGCTGTCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA754210.10435045992872329No Hit
GCCTCTCTCACCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG749090.10364207054800033No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC742130.10267910373358007No Hit
TTCACGTCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA741950.10265419941941402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCAC741060.10253106142159304No Hit
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA730380.10105340544774123Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)