FastQCFastQC Report
Fri 30 Jun 2023
SRR1840415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82331167
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1527984018.55899844101566Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT27580033.3498893559956464No Hit
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT24371492.9601778874335642No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT19780182.402514226477562No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10086571.2251217087691713No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA9487561.1523655434156546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8537831.0370106863661972No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG7920840.9620706578834234No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7504710.9115272227344962No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6067960.7370185825253758No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5707310.6932137862202293Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC5153930.6259998719561451Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5098600.6192794522152225No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4753880.5774095246336056No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4480540.5442094607987277No Hit
CCCCTACCTGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4354580.5289102728253566No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3883660.4717120067057959No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3748080.4552443669357924No Hit
TCCACGTTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3670450.44581537390329956No Hit
TCCCCAGTGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3555220.43181945908771097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG3531180.428899544202987No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3317850.40298833611820417No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3252620.39506545558864725No Hit
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3228100.39208723957477726No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3084640.3746624896012952No Hit
ATCCCACAGCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2824950.3431203641265039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2782420.3379546411628053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2485410.30187960289691995No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2477930.30097107696772957No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2381170.2892185410174011No Hit
ATCCCATTCTTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2369060.28774765211332426No Hit
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2275050.27632913304872747No Hit
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2063830.2506742070108153Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2051240.24914501697759248Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2017470.24504328962080668No Hit
TTCTCGCAACACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1975980.24000388577025758No Hit
TTCACGTCGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1847180.22435974944943998No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1836820.22310141674537423No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1822430.2213535974778543Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1819900.22104630194298108No Hit
ACCCGGGCTCAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1775120.2156072924364111No Hit
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1672540.2031478552951885Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCTACCTGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1655260.20104901464593597No Hit
TCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1606120.19508043654962404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA1600330.19437717917930156No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1554770.1888434303378695No Hit
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1553710.1887146820110056Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1494640.1815399993054878No Hit
TGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1430820.17378837834279695No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1419720.17244016473129792No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC1399480.16998180045231231No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1375940.16712261591044858Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1362960.16554605620979476No Hit
TCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1358470.1650006977309091Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1288220.1564680845590346No Hit
TCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1178170.1431013360954789No Hit
TCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAGC1146180.13921580875927583No Hit
TCCTGCATGGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1144580.13902147166212278No Hit
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG1124440.13657525345170926No Hit
TCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1113070.13519424545506564No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA1107520.1345201386493161No Hit
TCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGC1090310.13242980024806403No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1054540.12808515151983696No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA1046690.12713168513692996No Hit
TCCTGCGTGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1032230.1253753636214096No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG1022980.12425185227849375No Hit
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1004240.12197567902808909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1003800.12192223632637199Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCGGAATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC999710.12142546212177462No Hit
ACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC949420.11531720423688394No Hit
GTGTAGTGGTATCATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG921930.11197824998642374No Hit
TTCCCGGCTGTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT913950.11100899371437307No Hit
CTGTAGGCACATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC904350.109842971131455Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC899740.10928303737028289Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA887060.10774291587534524No Hit
TTCTCGCAACACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG871040.1057971156901007No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT863850.10492381335976933No Hit
TTGGCTGTAGTTTAGTGGTAAGAATTCCACGCTGTAGGCACCATCAATAG846890.10286384012994738No Hit
TCCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC841820.10224803445334377No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT828200.10059373991382875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)