FastQCFastQC Report
Fri 30 Jun 2023
SRR1840417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1840417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66026031
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT24260103.6743235406653474No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCCTGTAGGCACC24217383.6678533652886083No Hit
TCCCCTGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT13321912.017675422592038No Hit
TCCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT11528341.7460295318978056No Hit
TTCTCGGAATGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9492781.4377329450561704No Hit
TCCACGCTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9348791.4159248796887398No Hit
GCCTCTCTCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9019211.3660082036431964No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8892231.346776394904004No Hit
GTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC8833001.3378056906070879No Hit
ACCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8477501.2839632901756581No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG8072461.2226177884295362No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATACTCGGCTGTAGGCACCATCAATA7989861.2101075710578453No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC7452411.1287078576629874No Hit
GACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7118841.0781868745071168No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6948261.0523516096249976Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCAGCAGCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT6494120.9835696469472774No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG5975240.9049824606298082No Hit
TTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGC5807490.879575814575315No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5795670.8777856115567509No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG5576040.8445214585138398No Hit
ATCCGGCAGGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5418980.8207338708576924No Hit
TTCTCGGAATGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5407740.8190315119804793No Hit
GTCCGGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5272030.7984774974585402No Hit
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5043010.7637911780582419No Hit
CTCCCGGTAGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5036330.7627794558785458No Hit
ATCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4897930.7418180262872381No Hit
TTCCCCGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4348270.6585690422615287No Hit
ACCCGGGCTCAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4249170.643559810523822No Hit
ATCTCTCAGGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4027820.6100351541651807No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA4005350.6066319509649156No Hit
TTCCCCGGTTGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3614350.5474128832611489No Hit
TTCTCGGAATGCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3479050.5269209654598199No Hit
CTCCCGGCAAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3426440.5189528960176328No Hit
CCCCTACCTGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3417500.5175988845975007No Hit
ACCCGGTTGGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3170260.4801530475154564No Hit
TCCACGTTCACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3144390.47623489590037604No Hit
TCCCCAGTGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3017740.45705306744850377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTTGTAGTCTAGTTGGTCAGGATATTCGGCTGTAGGCACCATCAATA2962410.44867303927446434No Hit
ATCTCGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2944320.44593321079681436No Hit
ACCCTGCTGTCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2883330.43669594496752345No Hit
TGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGGCA2711100.41061077864880297No Hit
TCCGTTGTAGTCTAGTTGGTTAGGATATTCGGCTGTAGGCACCATCAATA2709350.41034573167058913No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAAT2594210.39290715505828305No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG2555510.3870458304543552No Hit
TTTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGCTGTAGG2547930.3858977984001492No Hit
GCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTTCTGTAGGCACCATCAAT2283070.3457833168860324No Hit
TTCCCGGCAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2208540.33449534472244746No Hit
ACGGTACTGACCGGGGTTCGTTTCCCCGGTTGCGCCTGTAGGCACCATCA2148450.32539438876766646No Hit
GCCCCACGGTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2059050.3118542745663449No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG2020520.30601869738315784No Hit
TTCCCGGCAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1922690.2912018140239264No Hit
TCCCCAGTGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1865850.28259308817154255No Hit
TTCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1684360.2551054446995307No Hit
ATCCCAGCAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1683720.25500851323321255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCCACTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1640930.24852773597734507No Hit
CCCCTACCTGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1607730.24349941616208917No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCTGTAGGCA1595960.2417167859143313No Hit
ACCTCAGGTCAGGCGGGGCTACCCGCTGAGCTGTAGGCACCATCAATAGA1555100.2355283176115796No Hit
TGCGAGAGGCACGGGGTTCGTTTCCCCGCACCTCCCTGTAGGCACCATCA1544900.2339834723671335No Hit
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG1520250.23025009635972213No Hit
AGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCACTGTAGGCAC1490680.2257715597049897No Hit
TTGGGTTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGTCC1443860.2186804171221499No Hit
GTCTAGTGGTAGAATAGTACCTGTAGGCACCATCAATAGATCGGAAGAGC1423040.21552711535848643No Hit
ATCTCTCAGGCGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1356420.20543715553642772No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1333000.20189006969084664No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAG1332610.201831002078559No Hit
ACCACGTGGTCGTGGGTTCGTTTCCCACAGACGGCGCTGTAGGCACCATC1180350.17877040041979808No Hit
GTCTAGTGGTCAGGACATTGGCTGTAGGCACCATCAATAGATCGGAAGAG1177560.17834783980881722No Hit
ATCCTGCTGTCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1171540.17743607820376178No Hit
GCCCTCCTTCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1154380.17483710326310542No Hit
GCTCTCGTAGCTCAGTTGGTTAGAGCACCCGTCTGTAGGCACCATCAATA1074940.1628054850063606No Hit
ACCCGGGCTCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1045380.1583284629057894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1026070.15540385882047036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT990690.15004536619806816No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCGGCTGTAGGCACCATCAATA985480.14925628348007167No Hit
TTCCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT983970.1490275858017272No Hit
TTCTCGCAACACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT980700.1485323265910077No Hit
ATCCCACAGCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT978920.14826273595031025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG969560.1468451132554068No Hit
TTCTCGCAACACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG952470.144256740193879No Hit
ACGGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCCTGTAGGCACCATCA902610.13670517314602781No Hit
CTCTGGTTCAAGTCCAGGATGGCCCCTGTAGGCACCATCAATAGATCGGA895120.1355707720792728No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG890360.13484984429853128No Hit
ACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC883510.13381237469809446No Hit
TCCTGCGTGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT878620.13307175771325705No Hit
TTCCCCGCGTCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT860070.1302622597441909No Hit
GCCCCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT842040.12753151859150824No Hit
TTCCCCGCAACTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT838220.12695295890192157No Hit
TCCTGCGTGGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT830330.1257579756687177No Hit
TCCTGCATGGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT806160.12209729826104494No Hit
TTCCCCGGATGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT803260.12165807755429067No Hit
ACTGAAGGTCTCCGGTTCGGGTCCGGGCGACGCCCTGTAGGCACCATCAA793810.1202268238719362No Hit
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC781730.11839724244518043No Hit
GCCTCGCCGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT771720.11688117372979757No Hit
GCCCTCCTTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC770940.11676303850522228No Hit
ACTGAAGGTCTCCGGTTCGTGTCCGGGCGACGCCCTGTAGGCACCATCAA764120.11573011256726912No Hit
ATCCCACTTCTGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT750580.11367940623297498No Hit
GCCCTCCTTCTAGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC750240.11362791139149346No Hit
ATCCTGCCGTTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT745620.11292818736900906No Hit
ACCTCGGTGGGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT741430.11229358917545718No Hit
ATCCTGCCGTCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT738470.11184528114373557No Hit
GAGACCTCAGGTCAGGCGGGGCTACCCGCTGAGTCTGTAGGCACCATCAA736640.111568117732232No Hit
GTCCGGGCGAAGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT736390.11153025387820145No Hit
TTGCGAGAGGTACGGGGATCGATACCCCGCATCTCCCTGTAGGCACCATC724620.10974762363044356No Hit
TGGGTTGGGTCGATCGGTCCGCCTCCGGTGTGCACCGGTCGGCTCGTCCC719600.10898731744151031No Hit
TTCACGTCGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG695120.1052796888548397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACGTGGTCGTGGGTTCGTTCCCCACAGACGGCGCTGTAGGCACCATC695020.10526454331322747No Hit
CTCCCACTGTGGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG691490.10472990569431623No Hit
TTCACGTCGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT672750.10189163119618685No Hit
GCCTCTCTCACCCCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC670690.10157963303897519No Hit
ACCTTGAGGTCACGGGTTCAAATCCCGTCTCCGCCTGTAGGCACCATCAA664860.10069664796298296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)