FastQCFastQC Report
Sat 23 Sept 2023
SRR18488793_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18488793_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43705832
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1850450.423387432597096No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC962780.22028639107018944Illumina Single End Sequencing Primer (100% over 33bp)
GCATATTCCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA663070.15171201866149123Illumina Single End PCR Primer 1 (100% over 27bp)
GCATACATCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA576350.13187027305646531Illumina Single End PCR Primer 1 (100% over 27bp)
GCATATTTCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA512240.1172017501005358Illumina Single End PCR Primer 1 (100% over 27bp)
GCATATACCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA463420.10603161610102743Illumina Single End PCR Primer 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)