FastQCFastQC Report
Sat 23 Sept 2023
SRR18488794_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18488794_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43862111
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC8815002.0097071935274617Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1330130.303252618187939No Hit
GCATATTCCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA696060.1586927724477283Illumina Single End PCR Primer 1 (100% over 27bp)
GCATATTTCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA639070.14569978175469028Illumina Single End PCR Primer 1 (100% over 27bp)
GCATACATCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA558850.12741064833838026Illumina Single End PCR Primer 1 (100% over 27bp)
GCATATGTCTATGCAGACTGGACAGATCGGAAGAGCGTCGTGTAGGGAAA512520.11684800122821266Illumina Single End PCR Primer 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)