FastQCFastQC Report
Sat 23 Sept 2023
SRR18488811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18488811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91550973
Total Bases13.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC89190779.74219793382207Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6713210.7332756583591962No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGAT5740320.6270080821533158Illumina Single End PCR Primer 1 (97% over 35bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGAT1063560.11617134861035283Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)