FastQCFastQC Report
Thu 21 Sept 2023
SRR18584182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20757938
Total Bases533.8 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAGTGATGATCATACTCGGATT3723881.7939546789281287No Hit
TGATGTGATGATAAACCTGGACAGAAA2456671.183484602372355No Hit
GTAAATGATGATCTTACTCGGATT2055890.9904114753594505No Hit
TGAGAATTTCTGGAAGGGCTCCCTC1880330.9058366009186463No Hit
GCAAGTGATGATCATACTCGGATT1879450.9054126667109228No Hit
TGGTCTGTGATGAAGTCGCCCGATT1245890.6001993068868401No Hit
TGATGTGATGATAAACCTGGACAGA1165100.5612792561573312No Hit
TGATGTGATGATAAACCTGGACAGAA977380.4708463817552591No Hit
TGGACGTCATAATTTGTGGTAGAGGG953650.4594146104492653No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT898680.43293317476909315No Hit
GTAAATGATGATCTTACTCGGATTC885160.4264200037595256No Hit
CAGCAAACGCACTCTGTCCCAAAACC841100.4051943887682871No Hit
TGCAAATCTAACGAAGTGCTCCTGGAC725270.3493940486767038No Hit
CGACATGAGGAGACTGAAACATCTGAAA715690.34477894673353393No Hit
AGCAAGTGATGATCATACTCGGATTC662120.31897195183837623No Hit
GGGGATGTAGTTCAGATGGTAGAAC649230.31276227918206523No Hit
ACTAAAGAATACCGTGTGTGCTCTGC617190.297327220073593No Hit
ATAAATGATGATTTTACTCGGATT570800.27497914291872344No Hit
TGGACGTCATAATTTGTGGTAGAGG549140.2645445804877151No Hit
AACGATGATGAAAAGCTCCGTGACG548180.26408210680656236No Hit
AACGATGATGAAAAGCTCCGTGACGC534380.257434047639992No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA490290.23619398034621744No Hit
GCAAGTGATGATCATACTCGGATTC468330.22561489488984887No Hit
CAGCAAACGCACTCTGTCCCAAAACCT462190.22265699030414293No Hit
AGGCCAAAGATGAAATATGTGGA417040.2009062749874289No Hit
AGAGTTCTACAGTCCGACGATCAG412310.198627628620916Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGATCCAG410300.1976593243510025Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCGTCTGTAGTCCAACGGTTAGGAT359010.17295070444858252No Hit
CAAAATGAGGAGTTCATATGATCTGAAG348180.16773342323307835No Hit
GGCAGATGATGAGAATCAAAGACA345710.16654351699094583No Hit
TCCCAATGAGGATCTCAAGACCGACCGAT342210.16485741502840986No Hit
TGATGTGATGATAAACCTGGACAG335220.1614900285375166No Hit
CGGGAAATGATGAATGAAACATTG320630.15446139207083093No Hit
TGGACGTCATAATTTGTGCTAGAGGG314660.15158538386616244No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA313180.15087240360771864Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGCCGAAGACGAAATATATGGA300390.14471090529319433No Hit
AGCAAGTGATGATCATACTCGGATC266660.12846169980852626No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT261580.12601444324575978No Hit
TGCGAGGTGATGATGCATGTATGA258130.12435242845411716No Hit
GACCATGAGGATATGCAAATCTGATCCT254960.12282530181947744No Hit
TGGTCTGTGATGAAGTCGCCCGAT244290.11768509955083206No Hit
TGACAAGACTGGGCAATACTGAGGCCT233520.11249672294039996No Hit
AGCAAGTGATGATCATACTCGGAT227210.10945692197365654No Hit
AACGATGATGAAAAGCTCCGTGACGCA218780.10539582496103417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)