FastQCFastQC Report
Thu 21 Sept 2023
SRR18584183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22988727
Total Bases608.3 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATGTGATGATAAACCTGGACAGAAA3982501.7323708267969775No Hit
AGCAAGTGATGATCATACTCGGATT3537651.5388629392136417No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA2780691.2095885083154017No Hit
CGACATGAGGAGACTGAAACATCTGAAA2424801.0547778482905992No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT1540400.670067550934856No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT1346310.5856392135153895No Hit
CAAAATGAGGAGTTCATATGATCTGAAG1298450.5648203138868889No Hit
GTAAATGATGATCTTACTCGGATTC1275140.554680561476936No Hit
TGAGAATTTCTGGAAGGGCTCCCTC1198620.5213946818368846No Hit
AGCAAGTGATGATCATACTCGGATTC1188920.5171752224470716No Hit
TGCAAATCTAACGAAGTGCTCCTGGAC1179810.5132124105871544No Hit
TCCCAATGAGGATCTCAAGACCGACCGAT1153700.5018546698997295No Hit
TGGACGTCATAATTTGTGGTAGAGGG1129390.49127992167639384No Hit
TGATGTGATGATAAACCTGGACAGAA1065120.4633227407502816No Hit
TGGTCTGTGATGAAGTCGCCCGATT888650.38655902956261995No Hit
TGATGTGATGATAAACCTGGACAGA872390.37948599763701574No Hit
GGGGATGTAGTTCAGATGGTAGAAC724070.31496741859607974No Hit
ACTAAAGAATACCGTGTGTGCTCTGC701720.3052452621669743No Hit
GTAAATGATGATCTTACTCGGATT681970.2966540948526641No Hit
GCGACATGAGGAGACTGAAACATCTGAAA655430.2851093059654847No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCG633540.2755872476105354No Hit
GCAAGTGATGATCATACTCGGATTC614400.2672614277423887No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT571280.2485044082693226No Hit
GCAAGTGATGATCATACTCGGATT520280.22631962178680012No Hit
CAGCAAACGCACTCTGTCCCAAAACCT510410.22202621310871193No Hit
TGGACGTCATAATTTGTGGTAGAGG494890.21527507808501095No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT488460.2124780550049596No Hit
TACTAAAGAATACCGTGTGTGCTCTGC472620.20558772132097614No Hit
ATCCGTGATGCAAGACAATTTCCTGAAC436380.18982347304398367No Hit
TTGTCGTCTAGTCCGGTTAGGATATCTGG432350.18807043991605105No Hit
GACCATGAGGATATGCAAATCTGATCCT429190.1866958531457614No Hit
CTGAGAATTTCTGGAAGGGCTCCCTC396280.17238014092733364No Hit
AACGATGATGAAAAGCTCCGTGACGC386880.16829118028153536No Hit
TGCCGGTGATGACAGCGTATGGCATGAGT371580.16163574433677863No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATA349460.15201363694475123No Hit
GCGTCTGTAGTCCAACGGTTAGGAT316660.1377457742657956No Hit
TGGACGTCATAATTTGTGCTAGAGGG315120.13707588071318608No Hit
GCGTCTGTAGTCCAACGGTTAGGATA313240.1362580885840264No Hit
CAGCAAACGCACTCTGTCCCAAAACC304850.1326084737097448No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA290100.12619228546234856Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCCGTGATGCAAGACAATTTCCTGAAC289010.12571814002576134No Hit
AACGATGATGAAAAGCTCCGTGACG282690.12296896648518207No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT267340.11629178074975617No Hit
AGCAAGTGATGATCATACTCGGATC264310.1149737434352063No Hit
TGGTCTGTGATGAAGTCGCCCGATTTGG263370.11456484737062647No Hit
GCCCGTCTAGCTCAGTTGGT244390.10630862683262104No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT232050.10094077849547736No Hit
TGGTCTGTGATGAAGTCGCCCGATTTG231600.10074503037945512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)