FastQCFastQC Report
Thu 21 Sept 2023
SRR18584184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19617041
Total Bases520.3 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGAT17664519.004676087489443No Hit
GCGTCTGTAGTCCAACGGTTAGGAT6706523.418721508508852No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT3819991.9472814477983709No Hit
AGCAAGTGATGATCATACTCGGATT2865061.460495494707892No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC2752311.403019955965836No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT2688401.3704411383959487No Hit
TGATGTGATGATAAACCTGGACAGAAA1776960.9058246857923171No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAC1740570.8872744875233731No Hit
GGTGTCGTGGTGTAGTTGGTTATC1597100.8141390946779384No Hit
GTAAATGATGATCTTACTCGGATT1347680.6869945370456227No Hit
TCCGTTGTAGTCTAGGTGGTTAGGAT1330560.6782674308525939No Hit
AACGATGATGAAAAGCTCCGTGACGC1277780.6513622518299268No Hit
CAAAATGAGGAGTTCATATGATCTGAAG1028570.524324743981521No Hit
GCGACATGAGGAGACTGAAACATCTGAAA942200.48029669714204093No Hit
CGACATGAGGAGACTGAAACATCTGAAA936400.47734008406262696No Hit
GCAAGTGATGATCATACTCGGATT838210.4272866636716516No Hit
GGGGATGTAGTTCAGATGGTAGAAC822340.4191967585733241No Hit
TCCGTTGTAGTCTAGTTGGTCAGGAT790780.403108705334306No Hit
TGATGTGATGATAAACCTGGACAGAA771030.39304092803802576No Hit
GTCTGGGTGGTGTAGTTGGTTATC737510.37595374348251603No Hit
TGATGTGATGATAAACCTGGACAGA712520.36321481919724796No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT653670.33321539165871145No Hit
GCGTCTGTAGTCCAACGGTTAGGAC648790.3307277585849976No Hit
GCACCAGTGGTCTAGTGGTAGAATAGT632950.32265314631294295No Hit
GTAAATGATGATCTTACTCGGATTC632360.3223523873962439No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT551940.28135741776754203No Hit
TGGACGTCATAATTTGTGGTAGAGGG517780.26394398625154525No Hit
TCCGTTGTAGTCTAGCTGGTCAGG502610.25621091376625044No Hit
TGGTCTGTGATGAAGTCGCCCGATT481150.2452714453724188No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCC476390.24284498360379633No Hit
GGTTTCGTGGTGTAGTTGGTTATC447150.22793957559654382No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA445780.22724120319675126No Hit
AGCAAGTGATGATCATACTCGGATTC425840.21707657133407635No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAC399940.20387376465186569No Hit
AACGATGATGAAAAGCTCCGTGACG345040.17588789257258522No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCT325420.16588638419015386No Hit
GGTGTCGTCGTGTAGTTGGTTATC313240.15967749672338452No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT310210.1581329212698286No Hit
ACTAAAGAATACCGTGTGTGCTCTGC308150.1570828138657609No Hit
GGTTTCGTCGTGTAGTTGGTTATC305760.15586448537269204No Hit
TGAGAATTTCTGGAAGGGCTCCCTC279770.14261580021166292No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT270870.13807892841738975No Hit
AACGATGATGAAAAGCTCCGTGACGT267420.13632025339601422No Hit
AGCAAGTGATGATCATACTCGGATC266520.1358614686078293No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAC266370.13578500447646513No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT265580.13538229338461394No Hit
CAGCAAACGCACTCTGTCCCAAAACC264560.13486233729133768No Hit
ATAAATGATGATTTTACTCGGATT257170.13109520441946368No Hit
GTTTTCGTGGTGTAGTTGGTTATC254790.1298819735351524No Hit
TGATGTGATGATAAACCTGGACAG246180.12549293239484996No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC244150.12445811781705508No Hit
GCGTTTGTAGTCCAACGGTTAGGAT243020.12388208802744513No Hit
GCAAGTGATGATCATACTCGGATTC239450.12206224170097824No Hit
TGGACGTCATAATTTGTGGTAGAGG220510.11240737071406437No Hit
ATCCGTGATGCAAGACAATTTCCTGAAC216840.1105365483000214No Hit
GCGTCTGTAGTCCAACGGTTAGG212490.10831908849046092No Hit
AATCCGTGATGCAAGACAATTTCCTGAAC211330.10772776587457812No Hit
TGGTCTGTGATGAAGTCGCCCGATTTGG207330.10568872237153401No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAG204840.10441941779088905No Hit
TGCAAATCTAACGAAGTGCTCCTGGAC203000.10348145777948876No Hit
CGAGGTGTAGCGCAGTCTGGTCAGCGC201710.10282386624975703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)