FastQCFastQC Report
Thu 21 Sept 2023
SRR18584185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25193438
Total Bases677.8 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAGTGATGATCATACTCGGATT2405950.9549907400490556No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT2280520.905203966207391No Hit
GCGACATGAGGAGACTGAAACATCTGAAA1699400.6745407276291548No Hit
TGGTCTGTGATGAAGTCGCCCGATT1586690.6298028875614357No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT1411540.5602808159807328No Hit
TGGACGTCATAATTTGTGGTAGAGGG1287670.5111132509981369No Hit
CGACATGAGGAGACTGAAACATCTGAAA1262570.5011503392272225No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA1188200.47163074765738605No Hit
TGATGTGATGATAAACCTGGACAGAAA1023130.40610971793528144No Hit
TGATGTGATGATAAACCTGGACAGA860810.3416802422916634No Hit
AACGATGATGAAAAGCTCCGTGACG812690.32258003056192647No Hit
GGGGATGTAGTTCAGATGGTAGAAC799090.31718179948286535No Hit
CAAAATGAGGAGTTCATATGATCTGAAG787860.31272428955508175No Hit
CAGCAAACGCACTCTGTCCCAAAACC717920.2849630923734982No Hit
TGAGAATTTCTGGAAGGGCTCCCTC702330.2787749730703685No Hit
AACGATGATGAAAAGCTCCGTGACGC669870.2658906656566682No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT624320.24781056082937153No Hit
TGGTCTGTGATGAAGTCGCCCGATTTGG606730.24082858401461524No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT508120.20168743940386383No Hit
GCGACATGAGGAGACTGAAACATCTGAA487840.1936377242359697No Hit
TGATGTGATGATAAACCTGGACAGAA478410.1898946860686501No Hit
TGGACGTCATAATTTGTGGTAGAGG477830.18966446739027837No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT471460.18713603121574754No Hit
GTAAATGATGATCTTACTCGGATT460310.18271027558842903No Hit
CAGCAAACGCACTCTGTCCCAAAACCT452420.17957850770506192No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT445540.1768476378650663No Hit
GCGTCTGTAGTCCAACGGTTAGGATA420540.1669244189697333No Hit
ACTAAAGAATACCGTGTGTGCTCTGC415380.1648762665897366No Hit
TGGTCTGTGATGAAGTCGCCCGATTTGGT394660.1566519027692846No Hit
GCAAGTGATGATCATACTCGGATT379010.15043996774080615No Hit
GTGCTGTAGATGATCGTTCCTGGTCAGG369070.14649449590802177No Hit
GCGTCTGTAGTCCAACGGTTAGGAT363330.14421612484965332No Hit
TTGTCGTCTAGTCCGGTTAGGATATCTGG352430.13988960141128814No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC332900.132137582810254No Hit
CAGAGTTCTACAGTCCGACGATCCAG332410.13194308771990548Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGACAAGACTGGGCAATACTGAGGCCT331320.13151043537606896No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT324310.1287279647978176No Hit
TGGACGTCATAATTTGTGCTAGAGGG323090.12824371171572535No Hit
TGGTCTGTGATGAAGTCGCCCGATTTG322070.12783884438479576No Hit
TCCCAATGAGGATCTCAAGACCGACCGAT299260.11878489946469395No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC296320.11761792892260277No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT287740.1142122801977245No Hit
AGAGTTCTACAGTCCGACGATCAG284260.11283096812749414Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAAGTGATGATCATACTCGGATC281200.11161636613470541No Hit
AATCCGTGATGCAAGACAATTTCCTGAAC279240.11083838577331129No Hit
TGCCGGTGATGACAGCGTATGGCATGAGT268240.10647216945936479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)