FastQCFastQC Report
Thu 21 Sept 2023
SRR18584188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19665416
Total Bases569.9 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATACT388701919.765760358184135No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG223549111.36762629379414No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA15511067.887481251350086No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGG8651134.399159417731107No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC5552682.823576170471044No Hit
GCGGAGTAGAGCAGTTTGGTAGCTCACGAGG5246412.6678357579620995No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA2987931.5193830631398797No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGA2824811.4364354153504812No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC2626601.335644259953616No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGA2367571.2039257140555786No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT2279451.1591160848059354No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTG2106891.0713681317496666No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACT1770370.900245385096354No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT1737360.8834595718697229No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT1640160.8340326998422002No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC1564490.7955539816701563No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACG1250150.6357099183663341No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT1219430.6200885859724503No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTGG1056890.5374358721930926No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGA1051160.5345221275766554No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG974940.49576373060198675No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA959910.48812087168661983No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGA937050.4764964036357024No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG914580.4650702532811917No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAG826990.4205301326958961No Hit
GGGGATGTAGTTCAGATGGTAGAAC825770.4199097542609828No Hit
CGCTGCAGCGCTGAAGTATGAGCCCCGTGG793580.40354091670371983No Hit
GTCAGGATAGCTCAGTTGGTAGAG789710.40157299494706855No Hit
GTCAGGATAGCTCAGTTGGTAGAGC743320.3779833592129452No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGA718730.3654791742010441No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC702830.35739391427061595No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATA691040.3513986177561665No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT642140.32653262966824603No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTT590660.300354693742558No Hit
GCGGAGTAGAGCAGTTTGGTAGCTCACGAGA573780.29177109703654375No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGT567900.2887810763830269No Hit
GGGTGTATAGCTCAGTTGGTAGAGCATTGGG521860.26536941806875586No Hit
TGTAGTCTAGCTGGTCAGGATATTCGG515240.2620031022989801No Hit
GGAGAGATGGCTGAGTGGACTAAAGC484050.24614277165558054No Hit
GGAGAGATGGCTGAGTGGACTAAAGCG456750.2322605329071096No Hit
GGGGATGTAGCTCAGATGGTAGAGCGCTCGC451600.22964172230071309No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC447330.22747039777851635No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC395550.20113990977866933No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCG378690.19256648321093234No Hit
AGGGCTATAGCTCAGTTCGGTAGAGC328660.16712588231034625No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGT324330.1649240473733177No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT298160.15161642143751244No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTT288230.14656694778284884No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATG285820.14534144612043803No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAC279200.14197513035066228No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAG276930.1408208196561924No Hit
TCCGTTGTAGTCTAGCTGGTCAGG274460.13956480757894976No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAC267510.13603068452759912No Hit
GCCCGTCTAGCTCAGTTGGTAGAGC263430.1339559763190364No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACC254760.1295472213758407No Hit
GTCAGGATAGCTCAGTTGGTAGAGT244330.1242434942642454No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTA243880.1240146661530069No Hit
GCGGAGTAGAGCAGTTTGGTAGCTCACGA243490.12381634845660015No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGT235210.11960591120981118No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCT235130.1195652306567021No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACC231300.11761764917660528No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAC224690.11425641847596817No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAG212420.1080170386428642No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTT202530.10298790526475515No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATA198820.10110134461432192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)