FastQCFastQC Report
Thu 21 Sept 2023
SRR18584194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8832000
Total Bases248.5 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATATT5921276.704336503623189No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA5685496.437375452898551No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC4067064.604913949275362No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT3893244.408106884057971No Hit
GCGTCTGTAGTCCAACGGTTAGGATA3378303.825067934782609No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT3176313.596365489130435No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGT1762741.9958559782608698No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT1715701.9425951086956523No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT1570001.7776268115942029No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTG1539191.7427423007246379No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC1362871.5431046195652174No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATT1106871.2532495471014493No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATC967591.0955502717391306No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT950901.0766530797101448No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC717470.8123528079710144No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC515970.5842051630434782No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA514900.5829936594202899No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATC479450.5428555253623188No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGA468390.5303328804347825No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA435230.4927875905797101No Hit
GCGTCTGTAGTCCAACGGTTAGGAT348540.39463315217391304No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT332070.3759850543478261No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC304310.34455389492753624No Hit
GGGTGTTTGGTCTAGTGGTATGATTCT303350.3434669384057971No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAC251560.2848278985507246No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAC250470.28359375No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT240120.271875No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTT231650.26228487318840576No Hit
GCGTCTGTAGTCCAACGGTTAGGATAA222880.25235507246376815No Hit
GGGAGAATCTTAGCGACAAAGGGCTA216890.2455729166666667No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC215500.24399909420289856No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAC205940.23317481884057972No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTA204770.23185009057971018No Hit
TGGACGTCATAATTTGTGGTAGAGG201310.22793251811594203No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT187530.21233016304347826No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATC177010.2004189311594203No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATA169450.19185914855072464No Hit
GGGTGTATAGCTCAGTTGGTAGAGCATTGGGC166830.18889266304347826No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATAT165060.18688858695652175No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT163670.1853147644927536No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATAT159720.18084239130434782No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTT157480.17830615942028985No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT150040.1698822463768116No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCT144400.1634963768115942No Hit
GTTGCTTCACGACTGGAAGAATGGCCT137050.155174365942029No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA131960.14941123188405797Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGGATGTAGTTCAGATGGTAGAAC128570.14557291666666666No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATA117450.13298233695652173No Hit
GCGTTTGTAGTCCAACGGTTAGGATA115300.13054800724637683No Hit
GGGTCCATAGCTCAGTGGTAGAGCAATTG113840.12889492753623188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC113560.12857789855072463No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTC109960.1245018115942029No Hit
TGGACGTCATAATTTGTGGTAGAGGG107120.12128623188405797No Hit
TCCGTTGTAGTCTAGCTGGTCAGAATATT106280.12033514492753622No Hit
GGGAGAATCTTAGCGACAAAGGGCTGAAC102360.11589673913043477No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG96980.1098052536231884No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC94190.10664628623188405No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT91420.10350996376811594No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG88510.1002151268115942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)