FastQCFastQC Report
Thu 21 Sept 2023
SRR18584204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918528
Total Bases109.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATATT3735619.533197159749783No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA2451816.256966901857024No Hit
GCGTCTGTAGTCCAACGGTTAGGATA1213983.0980511049046986No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT953862.434230404886733No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT831082.1208984598298137No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT675551.7239892122756302No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT647161.6515385369199862No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATT631891.6125698221372924No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATC365590.93297789373969No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC363490.9276187384650563No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT351880.8979902657324383No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC311600.7951965636075589No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA240060.6126280072517026No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC221930.566360633380698No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT182890.46673138484655463No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT179690.4585650529994937No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATAT176930.4515215917814036No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT174710.4458561990625051No Hit
GCGTCTGTAGTCCAACGGTTAGGAT168990.43125888088588366No Hit
GGGGATGTAGTTCAGATGGTAGAAC144290.3682250069413821No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC140930.3596503585019681No Hit
GCGTCTGTAGTCCAACGGTTAGGATAA129210.3297411681121074No Hit
GTGTGAAAGTAGGTTATCGTCAGGCT119800.30572704852434385No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT116310.296820642853643No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATAT114550.2923291603377595No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC110420.28178948829764644No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAC104040.2655078641775687No Hit
CCGACCTTAGCTCAGTTGGTAGA102720.26213925229065604No Hit
CCTCCTGGGAAGTCCTCGTGTTGCATTCC95730.24430092116223234No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG95460.24361188691263658No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA89260.22778961895895602Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCT87880.224267888349911No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAC79820.20369894001012623No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC79520.2029333463994643No Hit
GGGGATGTAGTTCAGATGGTAGAA78490.20030480833619155No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC72470.18494189654890816No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC70620.18022073594982604No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATA69440.17720940108122235No Hit
GGGTGTTTGGTCTAGTGGTATGATTCT68720.17537197641563362No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATA67180.17144192921423554No Hit
GCGTCTGTAGTCCAACGGTTAGGA67010.17100809283486046No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTC66480.16965554412269096No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATA62480.1594476293138648No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTG61460.15684461103761413No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATC61280.15638525487121696No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA60880.15536446339033433No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG59890.15283800447514984No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCC57940.1478616460058471No Hit
GCGTTTGTAGTCCAACGGTTAGGATA57670.14717261175625135No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAC56610.1444675143319124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT53520.13658190014209418No Hit
CAGAGTTCTACAGTCCGACGATCCAG53290.13599494504058668Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGTTGTAGTCTAGGTGGTTAGGATACT52180.1331622486811374No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA51320.13096754699723978No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGA50910.12992123572933512Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGTTCTACAGTCCGACGATC49930.12742029660117268Illumina DpnII expression Sequencing Primer (95% over 23bp)
TCCGTTGTAGTCTAGTTGGTCAGGATA49280.12576151044473843No Hit
AAGTTCTACAGTCCGACGATC47740.12183146324334035Illumina DpnII expression Sequencing Primer (95% over 21bp)
TGTGAAAGTAGGTTATCGTCAGGCT45610.11639574860764042No Hit
AGAGTTCTACAGTCCGACGATT45230.11542599670080193Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGAGTTCTACAGTCCGACGATCAG44810.11435416564587518Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGGCTGTAGTTTAGTGGTGAGAATT42620.10876533228804286No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTT42110.10746382314991752No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC40000.10207914808826173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)