FastQCFastQC Report
Thu 21 Sept 2023
SRR18584237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25789186
Total Bases723.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATACT22229598.619733092777725No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC9019273.497306971999814No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG6081432.358131815405108No Hit
TGATGTGATGATAAACATGGACAGAAA5449102.112939896590765No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA5391452.0905855655932686No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC4975621.929343562840642No Hit
GCGACATGAGGAGACTGAAACATCTGAAA4001931.5517860858423371No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT3890381.5085315217006072No Hit
CGACATGAGGAGACTGAAACATCTGAAA3462941.3427876319942786No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC3422351.32704847683056No Hit
AGCAAGTGATGATCATACTCGGATT2590601.0045295729768284No Hit
TCCCAATGAGGATCTCAAGACCGACCGAT2320630.8998461603247191No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC1798010.6971953283054378No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC1780630.6904560694548483No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT1551000.6014148721095733No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT1453210.5634958776907499No Hit
AGCAAGTGATGATCATACTCGGATTC1444940.5602891072250207No Hit
TGCAAATCTAACGAAGTGCTCCTGGACG1428380.5538678111050112No Hit
GGCAGATGATGAGAAGCAAAGACA1263070.4897673001389032No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACG1251630.4853313322878822No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT1250350.4848350002206351No Hit
GCAAGTGATGATCATACTCGGATTC1217970.4722793499569936No Hit
CATAATGAGGAAAACCTAGATTAGTCTGATC1176500.45619896649704256No Hit
AATCCGTGATGCAAGACAATTTCCTGAAC1174690.45549712193320097No Hit
GTAAATGATGATCTTACTCGGATTC1118000.43351503998613994No Hit
GCAAGTGATGATCATACTCGGATT1114430.43213073882983355No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG1106630.42910621529504656No Hit
CAAAATGAGGAGTTCATATGATCTGAAG1086450.421281230047354No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA954060.3699457594357573No Hit
GGGGATGTAGCTCAGATGGTAGAGCACTCGCTT952040.369162485392133No Hit
TCCATTGTCGTCCAGCCCGGTTAGGATACCTGGCT949100.3680224726751748No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC917750.35586621462189616No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACT844950.3276373282972173No Hit
ATCCGTGATGCAAGACAATTTCCTGAAC838980.32532240451482264No Hit
TGGAAATGAGGACCCTTTCCCCAGCTGATC777360.3014286685900051No Hit
AGGATATGATGGCTCAGTAAAAGGTCTGAG772270.29945497310384284No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTG743390.2882564808365801No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT735550.2852164469246916No Hit
TGATGTGATGATAAACATGGACAGAA730690.28333193610686275No Hit
GTAAATGATGATCTTACTCGGATT727330.28202906443033915No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA704750.2732734565565582No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCG684470.265409695366112No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTT678780.2632033442234276No Hit
AGCGACATGAGGAGACTGAAACATCTGAAA658190.25521937761044494No Hit
TGCGATGACGAGGTAAACACAACCCTGATC649780.25195832082486047No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCA642850.24927114799203048No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTG605650.23484649728766158No Hit
GGGGATGTAGCTCAGATGGTAGAGCACTCGC590150.2288362261608412No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATT583950.22643211771011307No Hit
CGGGAAATGATGAATGAAACATTGG574720.22285309819394844No Hit
TGGAGATGAGGATCCTTTCCCCAGCTGATC569260.22073593171959752No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTCG567860.220193068521046No Hit
TGATGTGATGATAAACATGGACAGA561480.21771916337336122No Hit
TCCCAATGAGGATCTCAAGACCGACCGATA553830.21475280375270472No Hit
TGCCGGTGATGACAGCGTATGGCATGAGTCT551210.21373687405255834No Hit
GGGGATGTAGTTCAGATGGTAGAAC507760.19688872692608445No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTC499990.19387583617412354No Hit
GCAAGTGATGATCATACTCGGATTCC498820.19342215764390547No Hit
AGCAAGTGATGATCATACTCGGATTCC481380.18665963322766374No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCG455930.1767911557968522No Hit
CTGGTTGATGATCCATGACCTGTGGG435320.16879943399531883No Hit
TGCAATGATGTCTTTCTCGTTAACCTGAG433000.16789983212343343No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG428200.1660385868712568No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATC419680.1627348765486433No Hit
TGGCGATGACGAAAAACTATGGTT403040.156282559674431No Hit
GCCCGTCTAGCTCAGTTGGT398470.1545104990905878No Hit
GCGTCTGTAGTCCAACGGTTAGGATA391860.15194740927456957No Hit
TGCCGGTGATGACAGCGTATGGCATGAGTC388840.15077637580340847No Hit
CATGATGAGGAAAGAAATACAAGTCTGATC381350.14787205769115783No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT371350.14399446341578986No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC363280.1408652448355679No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG350410.13587478100316933No Hit
ATCCCAATGAGGATCTCAAGACCGACCGAT343590.13323026170736835No Hit
CAGCAAACGCACTCTGTCCCAAAACCT338010.13106656410171302No Hit
TACAATGATGTCTTTCTCGTTAACCTGAG335700.13017083982410302No Hit
CGGGAAATGATGAATGAAACATTG317110.12296239206619394No Hit
CAAAATGAAGAGGTCTTGTAATCTGAAG308460.11960827301800066No Hit
TTGGAAAGCGAGGTGTGACTGTGTTCA307840.11936786217292784No Hit
AGCAATGATGAATCGCCCTTTGAATTAGA305520.11846826030104246No Hit
AGCAAGTGATGATCATACTCGGATC292640.1134739188743685No Hit
AACGATGATGAAAAGCTCCGTGACGCATGGGT282970.10972428521008766No Hit
TCCCAATGAGGATCTCAAGACCGACCGAC263260.10208154689333739No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATA260800.10112765870159686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)