FastQCFastQC Report
Thu 21 Sept 2023
SRR18584242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45806056
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATACT37537918.19496662188074No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC16396083.579456829900396No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA9191832.0066844436464906No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC9122431.9915336085691377No Hit
TGATGTGATGATAAACATGGACAGAAA5387511.176156707313985No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT4624981.0096874526809292No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC4323650.9439035746714364No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC3966160.8658593090834976No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG3836930.8376468823249048No Hit
TCCATTGTCGTCCAGCCCGGTTAGGATACCTGGCT2702350.5899547431020912No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTG2530800.5525033633107378No Hit
TCCCAATGAGGATCTCAAGACCGACCGAT2391860.5221711295117833No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCG2195800.4793689288595377No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT2130040.4650127485326394No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT2123790.4636483001286992No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC2101320.45874283522685294No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA2033530.4439434820583549No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT1958750.42761812979489006No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTT1919880.41913235228110446No Hit
AGCAAGTGATGATCATACTCGGATT1641210.3582954184049375No Hit
GTAAATGATGATCTTACTCGGATTC1543910.33705368565239496No Hit
GCGACATGAGGAGACTGAAACATCTGAAA1490150.32531724626106207No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACT1462910.3193704343373287No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG1419910.3099830293182194No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT1404510.30662102845091055No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCTG1359710.29684066229146644No Hit
CGACATGAGGAGACTGAAACATCTGAAA1215510.2653601087157558No Hit
AGCAAGTGATGATCATACTCGGATTC1156800.2525430261885022No Hit
GTAAATGATGATCTTACTCGGATT1145500.2500761034741782No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCT952280.207893908176683No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATA881460.1924330704219547No Hit
GCAAGTGATGATCATACTCGGATT867530.18939198781925254No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTG816670.17828865248734796No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA807320.17624743767505327Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTGTCGTCTAGTCCGGTTAGGATATCTGG803280.17536545822674626No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCT767130.16747348865835557No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT760810.16609375843229113No Hit
TGATGTGATGATAAACATGGACAGAA725770.15844411490044025No Hit
AATCCGTGATGCAAGACAATTTCCTGAAC671230.1465373923482956No Hit
GCAAGTGATGATCATACTCGGATTC653410.14264707705898103No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTA647960.14145727805074507No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA645500.14092023115895416No Hit
TCCCAATGAGGATCTCAAGACCGACCGATA603470.13174458853213647No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTC570600.12456868148613362No Hit
TGCAAATCTAACGAAGTGCTCCTGGACG569460.1243198060972549No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCC543900.11873975790450066No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACG527410.11513979723554457No Hit
GGCAGATGATGAGAAGCAAAGACA512540.11189350159288981No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTT493360.10770628233087783No Hit
TGGAAATGAGGACCCTTTCCCCAGCTGATC461050.10065262986186806No Hit
TGATGTGATGATAAACATGGACAGA459800.10037974018107998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)