FastQCFastQC Report
Thu 21 Sept 2023
SRR18584243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14299986
Total Bases386 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATA1352110.9455323942275188No Hit
AGCAAGTGATGATCATACTCGGATT1309340.9156232740367717No Hit
TGGACGTCATAATTTGTGGTAGAGGG1284890.8985253551996485No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT951590.6654482039353046No Hit
GCGTCTGTAGTCCAACGGTTAGGATA789200.5518886521986806No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT591340.41352488037400875No Hit
GGGGATGTAGTTCAGATGGTAGAAC577140.40359480072218246No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT461740.3228954210164961No Hit
TGCAAATCTAACGAAGTGCTCCTGGAC377640.26408417462786327No Hit
GCAAGTGATGATCATACTCGGATT357260.24983241242334084No Hit
TGGACGTCATAATTTGTGGTAGAGG346840.2425456920027754No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT304480.21292328537944025No Hit
AACGATGATGAAAAGCTCCGTGACG282170.19732187150393013No Hit
TGATGTGATGATAAACCTGGACAGAAA274390.19188130673694365No Hit
GCGTCTGTAGTCCAACGGTTAGGAT267270.18690228088335192No Hit
TGATGTGATGATAAACCTGGACAGAA243510.17028688000114126No Hit
ACGGATGCGATCATACCAGCACTAAAGC208470.14578335950818413No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT207300.14496517688898436No Hit
TGAACGACATACGCCTATCTGAGCTT204230.14281832164031488No Hit
TGATGTGATGATAAACCTGGACAGA198200.1386015342952084No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT195180.1364896441157355No Hit
AACGATGATGAAAAGCTCCGTGACGC185470.12969942767776138No Hit
TGACAAGACTGGGCAATACTGAGGCCT177750.12430082099381076No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT177570.12417494674470311No Hit
TGAGAATTTCTGGAAGGGCTCCCTC176420.12337075015318197No Hit
CGACATGAGGAGACTGAAACATCTGAAA165680.11586025328975846No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA160520.11225185814867232Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCATTGTCGTCTAGTCCGGTTAGGATAT151160.10570639719507417No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT143540.10037772064951672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)