FastQCFastQC Report
Thu 21 Sept 2023
SRR18584244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19289763
Total Bases510.4 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGATTTAGCTCAGCGGTAGAGCAGCTG3170721.6437319629069573No Hit
TGGACGTCATAATTTGTGGTAGAGGG3166121.641347278346551No Hit
AGCAAGTGATGATCATACTCGGATT2422721.2559615169973835No Hit
GGGGATGTAGTTCAGATGGTAGAAC1579810.8189888076903797No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT1555660.8064692137482457No Hit
AGAGTTCTACAGTCCGACGATCAG934940.4846819528057447Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGACGTCATAATTTGTGGTAGAGG882390.457439523751536No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA771340.3998701280052015No Hit
CAGAGTTCTACAGTCCGACGATCCAG720860.3737008070031757Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGGTGTAGTTCAATGGTAGAACAACGGG634130.3287391348457729No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGG596270.3091121440942535No Hit
AACGATGATGAAAAGCTCCGTGACG515580.26728166644660173No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTA463320.24018957620163608No Hit
TGATGTGATGATAAACCTGGACAGA461020.23899723392143282No Hit
AACGATGATGAAAAGCTCCGTGACGC417270.21631681011321913No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT409170.21211769164815553No Hit
TGGTCTGTGATGAAGTCGCCCGATT391630.20302478573738825No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT391530.20297294476868377No Hit
GGACGTCATAATTTGTGGTAGAGGG390200.20228345988491406No Hit
GCGTCTGTAGTCCAACGGTTAGGAT352110.18253723490537443No Hit
GCAAGTGATGATCATACTCGGATT332870.17256303252663083No Hit
GCGTCTGTAGTCCAACGGTTAGGATA319700.1657355769482497No Hit
TGGACGTCATAATTTGTGGTAGAG293980.15240207979745526No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT282930.14667365275560929No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC281090.1457197789314467No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA275750.14295147120262702Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACGGATGCGATCATACCAGCACTAAAGC263080.13638322046776832No Hit
TGCAAATCTAACGAAGTGCTCCTGGAC258880.1342058997821798No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCT258120.1338119084200257No Hit
TGGACGTCATAATTTGTGCTAGAGGG256940.13320018498931271No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT238920.1238584424287639No Hit
TGATGTGATGATAAACCTGGACAGAA234760.12170185813065718No Hit
TGATGTGATGATAAACCTGGACAGAAA225600.11695322539732604No Hit
CCGACCTTAGCTCAGTTGGTAGAG217200.112598584026149No Hit
TGGACGTCATAATTTGTGGCAGAGGG203700.10560005325104306No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT203050.10526308695446387No Hit
AGCAAGTGATGATCATACTCGGATC197740.10251033151625555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)