FastQCFastQC Report
Thu 21 Sept 2023
SRR18584245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13830652
Total Bases383.2 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATACT163055011.789393587518505No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA7775845.622178911015909No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT3406442.4629641465926553No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT2081891.5052724918536016No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT1850261.3377966562964638No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC1818391.3147536356203597No Hit
CGACATGAGGAGACTGAAACATCTGAAA1768201.2784646739719863No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC1664651.2035947401467408No Hit
GCGACATGAGGAGACTGAAACATCTGAAA1587871.1480803652640525No Hit
AGCAAGTGATGATCATACTCGGATT1181630.854355962394253No Hit
GCGTCTGTAGTCCAACGGTTAGGATA876290.6335854593116796No Hit
CAAAATGAGGAGTTCATATGATCTGAAG762690.5514490567762098No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT744920.5386007832457935No Hit
GGGTGTTTGGTCTAGTGGTATGATTCT740680.5355351287849626No Hit
TGCCATGATGGACTTCTCGTTAACCTGAGA719880.520496069165792No Hit
GTAAATGATGATCTTACTCGGATT706070.5105110012167178No Hit
TACAATGATGTCTTTCTCGTTAACCTGAG699150.5055076217664938No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC672610.48631836011780216No Hit
TTGCAAGATCAGCTTCTTGTTTGGACT668980.4836937550015719No Hit
TGTCCGGACATAGTCAGGTATCTGCCG654960.473556850392881No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCG629000.45478694713741624No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT613060.4432618216408019No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG599890.4337394939877021No Hit
AGGATATGATGACCCTTTCCCCAGATG597740.43218497580591286No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACT591640.4277744823599061No Hit
TGCAATGATGTCTTTCTCGTTAACCTGAG579850.4192499384700013No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACG573060.41434055314239704No Hit
AGGATATGATGGCTCAGTAAAAGGTC564910.40844784468584705No Hit
GTTGCTTCACGACTGGAAGAATGGCCT553190.3999739130158144No Hit
TGAGAATTTCTGGAAGGGCTCCCTC532930.3853252905213724No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT520960.37667060092322474No Hit
AACGATGATGAAAAGCTCCGTGACG475560.3438449611775352No Hit
GCAAGTGATGATCATACTCGGATT443390.32058503098769314No Hit
AGGATATGATGGCTCAGTAAAAGGTCTGAG427750.30927681500481685No Hit
GCCCGTCTAGCTCAGTTGGTAGAG402960.29135285885293044No Hit
GTTGCTTCACGACTGGAAGAATGGCCTG396970.2870218988952943No Hit
TCCCAATGAGGATCTCAAGACCGACCGAT380040.27478097200334445No Hit
GTTGCTTCACGACTGGAAGAATGGCC368100.2661479733565706No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA360510.26066016265899833No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT359060.2596117666759311No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC351240.253957658684493No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAC348410.2519114789382308No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA339590.24553433923433254No Hit
CCGACCTTAGCTCAGTTGGTAGAG327050.23646752156008263No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG317500.22956256870608846No Hit
GTGCTGTAGATGATCGTTCCTGGTCAGG311800.2254412879450658No Hit
GCCCGTCTAGCTCAGTTGGTAGAGC311360.2251231539915833No Hit
GCGACATGAGGAGACTGAAACATCTGAA301840.21823989208896297No Hit
CAACATGAGGAGGTCATACATTCTGAAG301650.21810251606359554No Hit
TGCGATGACGAGGTACACACAACCCTGATC296590.21444397559854736No Hit
TGGACGTCATAATTTGTGGTAGAGGG295710.21380770769158244No Hit
GTTGCTTCACGACTGGAAGAATGGC292180.2112554057465982No Hit
TGGTCTGTGATGAAGTCGCCCGATT283400.20490718731119834No Hit
AATCCGTGATGCAAGACAATTTCCTGAAC270100.19529086553547872No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATA263220.1903164073537531No Hit
CGACATGAGGAGACTGAAACATCTGAA253600.18336084227988672No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATC250640.18122066841100476No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACC249620.18048317606429545No Hit
ATAAATGATGATTTTACTCGGATT249030.18005658735394398No Hit
GGGGATGTAGTTCAGATGGTAGAAC248500.1796733805463401No Hit
TTGGGACTAATACGTTGCCCCGATGGCA245820.17773565555694698No Hit
TGGCGATGACGAAAAACTATGGTT240430.17383851462678693No Hit
ATGGGACTGAAGTTTGCCTCTCTGGCTT239030.17282627022934274No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC236070.1706860963604608No Hit
GCGTCTGTAGTCCAACGGTTAGGAT234000.16918942071566834No Hit
GTCCGGTTATAGTCAGGTATCTGTCTT228120.16493799424640285No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATT224990.16267490498640266No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACC218970.15832225407739273No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCC217000.1568978816038463No Hit
ATCCGTGATGCAAGACAATTTCCTGAAC213410.15430219775611445No Hit
TGCCGGTGATGACAGCGTATGGCATGAGT209550.1515112953460184No Hit
AATCCGTGATGCAAGACAATTTCCTGA202210.14620424257656112No Hit
TGCCGATGATGGTGTTAAATATACTTTG191210.13825089373949978No Hit
ATGGGGCTGGAGTTTGGCTCTTTGGCT185270.13395608536748665No Hit
CAGCAAACGCACTCTGTCCCAAAACC184200.13318244143515431No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAC183840.13292215001866867No Hit
TGTTGCTTCACGACTGGAAGAATGGCCT183640.13277754367617664No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT171930.12431084232326864No Hit
ATGGGGCTGGAGTTTGGCTCTTTGGCTT170260.12310337936346023No Hit
GGGGATGTAGCTCAGATGGTAGAGCGCTCGC169690.12269125128735797No Hit
TGGAGATGAGGATCCTTTCCCCAGCTGATC164900.11922792938467398No Hit
TTGCAAGATCAGCTTCTTGTTTGGACC163980.11856274020921066No Hit
GTAAATGATGATCTTACTCGGATTC162950.11781801754537674No Hit
GGGCATTTGGTCTAGTGGTATGATTCTCGC162540.11752157454326809No Hit
AACGATGATGAAAAGCTCCGTGACGC160750.11622734777796448No Hit
ACGGATGCGATCATACCAGCACTAAAGC158910.11489696942703785No Hit
TCCGTTGTAGTCTAGTTGGTCAGGAT156370.11306046887738916No Hit
GCACCAGTGGTCTAGTGGTAGAATAGT154440.11166501767234112No Hit
TGTCCGGTTATAGTCAGGTATCTGTCTT149840.10833907179502456No Hit
AACGAGGGACCTATGGATGGACTGCT149580.10815108354978492No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC146160.1056783150931713No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG145910.1054975571650563No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGA145870.10546863589655789Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGTCTGTAGTCCAACGGTTAGGATAA145270.10503481686908181No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATAT144650.10458653720735653No Hit
TGATGTGATGATAAACCTGGACAGAAA144220.10427563357099869No Hit
AGGATATGATGGCTCAGTAAAAGGTCTG143730.10392134803189322No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC142460.10300309775706885No Hit
GTCCGGACATAGTCAGGTATCTGCCG139550.1008990754738099No Hit
AAGCACATATCGAAGGCTTTGTAGGC139350.10075446913131787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)