FastQCFastQC Report
Thu 21 Sept 2023
SRR18584246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26464717
Total Bases767.2 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATACT364913113.788664356395724No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC20086587.589947022671733No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG18251456.896521886102164No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC9850923.722284277591179No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC7801302.9478116089433337No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC5684322.147886183706404No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA5626002.1258492958757125No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT4011471.515780425689041No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC2697841.0194101074271833No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACG2375850.8977424546047479No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC1932330.7301532829540553No Hit
CATAATGAGGAAAACCTAGATTAGTCTGATC1853180.7002455382387047No Hit
GCGACATGAGGAGACTGAAACATCTGAAA1794700.6781481925538823No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTG1680320.634928384082097No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT1502970.5679146313939424No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCA1382690.5224654395510824No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCG1367550.5167446151039514No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT1335140.5044981210265729No Hit
CGACATGAGGAGACTGAAACATCTGAAA1222030.4617581967719511No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA1161360.43883333420871273No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACT987880.37328190586734783No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT978680.36980557925482443No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTT947060.35785759583221693No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG901340.340581764014329No Hit
GGGTGTTTGGTCTAGTGGTATGATTCT878590.3319854128801No Hit
GCGTCTGTAGTCCAACGGTTAGGATA859720.3248551647085438No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTCG852020.3219456304784971No Hit
AGCAAGTGATGATCATACTCGGATT847140.32010166592750644No Hit
AGGATATGATGGCTCAGTAAAAGGTC807240.30502498855362786No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT803890.30375915223276334No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGT757270.28614324498538946No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGC752910.284495768460324No Hit
TTGCAAGATCAGCTTCTTGTTTGGACT749520.28321481767592677No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCG741670.28024860420763237No Hit
AGGATATGATGGCTCAGTAAAAGGTCTGAG717820.2712366053262538No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT692190.26155201281766965No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGG625160.23622395055273027No Hit
TGCCATGATGGACTTCTCGTTAACCTGAGA624030.23579696695793118No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC612600.2314780090034592No Hit
TGCAATGATGTCTTTCTCGTTAACCTGAG572960.21649957564254327No Hit
CAAAATGAGGAGTTCATATGATCTGAAG555540.2099172267740479No Hit
GTAAATGATGATCTTACTCGGATT535480.20233732331239362No Hit
GTTGCTTCACGACTGGAAGAATGGCCT524330.1981241666026506No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCG509570.19254692955908048No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTG501900.18964873117668327No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTGG501110.18935022052191225No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT498450.188345108696987No Hit
TACAATGATGTCTTTCTCGTTAACCTGAG492930.18625931272947296No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTC480320.18149447810078603No Hit
GTGCTGTAGATGATCGTTCCTGGTCAGG468560.1770508258221692No Hit
TGAGAATTTCTGGAAGGGCTCCCTC464910.17567163102480937No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCC453910.17151515355331404No Hit
TGTCCGGACATAGTCAGGTATCTGCCG448210.1693613424999028No Hit
TTGGATGATGAAATGCACTGGGCTCCGAGC422090.15949159781304292No Hit
TGCAATGATGTCTTTCTCGTTAACCTGAGA417450.15773832004324853No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATC416580.15740958046141207No Hit
GGAGAGATGGCTGAGTGGACTAAAGCGG391750.1480272772234821No Hit
TGCAATGATGTCTTTCTCGTTAACCTGAGG387250.1463269000760522No Hit
CATGATGAGGAAAGAAATACAAGTCTGATC379660.1434589306207204No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACGC379330.14333423629657555No Hit
GTTGCTTCACGACTGGAAGAATGGCCTG371350.14031890082179982No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTA367030.1386865387602671No Hit
GCAAGTGATGATCATACTCGGATT361570.13662341448805215No Hit
TACAATGATGTCTTTCTCGTTAACCTGAGA358070.13530089892893998No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC355310.13425800094518298No Hit
GTTGCTTCACGACTGGAAGAATGGCC346970.13110663529861286No Hit
AACGATGATGAAAAGCTCCGTGACG339060.1281177501350194No Hit
CTGCCATGATGGACTTCTCGTTAACCTGAGA337190.1274111489648652No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATT336880.12729401187248668No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA335490.126768784264725No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG330940.12504951403787917No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACC326730.12345871675106142No Hit
AGGATATGATGACCCTTTCCCCAGATG325670.1230581834674446No Hit
GCGTCTGTAGTCCAACGGTTAGGAT317950.1201410920056315No Hit
GTTGCTTCACGACTGGAAGAATGGC313920.11861830980471093No Hit
GGGGATGTAGTTCAGATGGTAGAAC293070.11073989568828564No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACC277460.10484147629464544No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCTG274370.10367388398674356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)