FastQCFastQC Report
Thu 21 Sept 2023
SRR18584249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8108889
Total Bases208.6 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAATGATGATCTTACTCGGATT1767552.179768399838745No Hit
AGCAAGTGATGATCATACTCGGATT1469711.812467774561965No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT955741.1786324859052824No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA862301.0634009171909986No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACT830451.0241230333773221No Hit
GCAAGTGATGATCATACTCGGATT794080.979271019741422No Hit
ATAAATGATGATTTTACTCGGATT604230.7451452350624111No Hit
GCGTCTGTAGTCCAACGGTTAGGATA562510.6936955235174634No Hit
TGGTCTGTGATGAAGTCGCCCGATT441880.5449328508504679No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT383650.47312276687965515No Hit
TGACAAGACTGGGCAATACTGAGGCCT356640.4398136415481825No Hit
GTGCTGTAGATGATCGTTCCTGGTCAGG277520.3422417053680227No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAT259700.32026582186536284No Hit
GTAAATGATGATCTTACTCGGAT258070.3182556821285875No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC255150.31465469560626613No Hit
AACGATGATGAAAAGCTCCGTGACG189760.23401479536839137No Hit
GTAAATGATGATCTTACTCGGATC185690.2289956120992654No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT180690.22282953928707125No Hit
GCGTCTGTAGTCCAACGGTTAGGAT162510.2004096985419334No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC160470.1978939408345582No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATT160350.19774595508706555No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGC158650.19564949033091958No Hit
GCGACATGAGGAGACTGAAACATCTGAAA155070.19123458219738856No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAT152210.18770758854881353No Hit
AGCAAGTGATGATCATACTCGGATC151760.18715264199571605No Hit
GTTGCTTCACGACTGGAAGAATGGCCT145690.1796670296017124No Hit
CGTAAATGATGATCTTACTCGGATT143620.17711427545746403No Hit
GTAAATGATGATCTTACTCGGATTC142320.17551109652629357No Hit
CCGACCTTAGCTCAGTTGGTAGA141050.17394491403199625No Hit
TGAGAATTTCTGGAAGGGCTCCCTC132160.1629816365719151No Hit
TGAACGACATACGCCTATCTGAGCTT131940.16271032936817856No Hit
AGCAAGTGATGATCATACTCGGAT129000.1590846785546084No Hit
ACTAAAGAATACCGTGTGTGCTCTGC110760.13659084493572424No Hit
TGATGTGATGATAAACCTGGACAGA106480.13131268660848608No Hit
CGACATGAGGAGACTGAAACATCTGAAA104930.12940120403670588No Hit
TGATGTGATGATAAACCTGGACAGAAA104470.12883392533798402No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC104450.12880926104673524No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC98080.12095368428399994No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATC95780.11811729079039064No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT90920.11212386801693795No Hit
ACGGATGCGATCATACCAGCACTAAAGC89900.11086598916325036No Hit
AGAACTCCGAAGTTAAGCGTGCTTGGGC89330.11016305686266023No Hit
TGACAAGACTGGGCAATACTGAGGCC87580.10800493137839227No Hit
TGGACGTCATAATTTGTGGTAGAGGG86880.1071416811846851No Hit
GCCGAGCTCTGTAGCGAGAGC85810.10582214160287555No Hit
GTTGCTTCACGACTGGAAGAATGGCC82220.10139490132372019No Hit
CCTGGGAAGTCCTCGTGTTGCATTCC81700.10075362975125199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)