FastQCFastQC Report
Thu 21 Sept 2023
SRR18584250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18584250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21148652
Total Bases631.8 Mbp
Sequences flagged as poor quality0
Sequence length20-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTTGTAGTCTAGCTGGTCAGGATACT19274889.113999322510011No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC5853852.7679541939599743No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGG5233242.4745028666602487No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTGG4352402.0580035077412973No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTC4171871.9726410931533604No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCG3391201.6035064551631943No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTC2984691.4112908945685996No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAT2959851.399545465119952No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACC2897521.3700731375219564No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGT2840911.3433054740321038No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATA2615101.2365327113992892No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG2364871.1182131135355577No Hit
GGGGATGTAGTTCAGATGGTAGAAC2102460.9941342833576344No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACA2045860.9673713483015371No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1751260.8280716898646779No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTT1588810.7512582835066746No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1576490.7454328531199057No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACT1460400.6905404656523735No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTC1450710.6859586133432997No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCTGG1417500.6702554848413034No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGA1229410.5813183743342129No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTC1169900.5531794650552668No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCTG1144500.5411692433163116No Hit
TCCATTGTCGTCCAGTCCGGTTAGGATACCT1138170.5381761447490837No Hit
TGTAGTCTAGCTGGTCAGGATATTCGG1117020.5281755073562135No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT914970.4326375033264531No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACT890680.4211521377343578No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATT877110.4147356531281521No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTG818290.38692300577833516No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTT778270.3679998138888474No Hit
AGCGGAGTAGAGCAGTTTGGTAGCTCACGAGA730670.345492469212695No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC711510.3364327901371681No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCA710020.33572825350759944No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC684550.3236849327323557No Hit
GGGGATGTAGTTCAGATGGTAGAAT681560.3222711310394629No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTG677920.3205499811524631No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGA670900.31723062065610613No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAT664960.3144219310053426No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT651850.3082229543518897No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCTGA623730.29492659863143994No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGT620430.2933662154921269No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATATCT598650.2830676867726605No Hit
TTGTAGTCTAGCTGGTCAGGATATTCGG584470.2763627677073697No Hit
ATCCACGGCCATAGGACTCCGAAAGCACCGCATCCCGT558730.2641917792207277No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTT554350.2621207252358212No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCT528540.24991663771289063No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATT520740.24622845938360513No Hit
GCCGGGATAGCTCAGCTGGGAGAGCAACAGACT484760.229215554731337No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC471830.223101689885483No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTA446940.21133261826805794No Hit
TCTCTGTTAGTATATCGGTTAGTACACGCGCCTG434300.20535587800111327No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTT433940.20518565438591546No Hit
TCCATTGTCGTCTAGTCCGGTTAGGAC411190.19442846759216617No Hit
GCGGAGTAGAGCAGTTTGGTAGCTCACGAGG401670.18992699865693566No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA394280.18643268611162547No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGTGG377100.17830923691968642No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACG369970.17493786365201905No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG356100.1683795260331486No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT334990.15839780237530032No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGA325050.1536977392223391No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC323630.15302630162905892No Hit
TCCATTGTCGTCCAGCCCGGTTAGGATACCTG308860.14604240497219398No Hit
GGGCCTGTAGCTCAGAGGTTAGAGCACGT307910.14559320376542204No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGC298860.14131397121669978No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG296480.14018860398289215No Hit
GCGGGGTAGAGGAATTGGTCGACTCATCAGG290840.1375217673447934No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGT281220.132973014072008No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC275220.13013595381871146No Hit
TGGGGCGTGGCCAAGTGGTAAGGCAGCGGG269500.12743128971056877No Hit
GCCCGTCTAGCTCAGTTGGTAGAG259110.12251844703861031No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC250620.11850400678019572No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGA248140.11733135520883317No Hit
GGGGATGTAGCTCAGATGGTAGAGCGCTCGC246800.11669774508559694No Hit
TTGTCGTCTAGTCCGGTTAGGATATCTGG231130.10928828939073754No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATA226410.10705646865814428No Hit
TGTCGTCTAGTCCGGTTAGGATATCTGG223840.10584126118298226No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC220650.10433289081497961No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACC216960.10258809875920223No Hit
TCTCTGTTAGTATATCGGTTAGTACACGCGCCT215170.10174170911696878No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCC213800.10109391369246608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)