FastQCFastQC Report
Thu 21 Sept 2023
SRR18590152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18590152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30496160
Total Bases856.7 Mbp
Sequences flagged as poor quality0
Sequence length10-134
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGCGGGCATGCCTGTTCGAGCGTCATT8626282.828644655589425No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCAT5570761.826708674141269No Hit
TAGCCCGTTGCACAATACCCTTCGGTGGGCTGAGG4260441.3970414635809887No Hit
TGGCGGGCATGCCTGTTCGAGCGTCATT4056871.3302887970157555No Hit
TACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTTC3795921.2447206467961869No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACA3771851.2368278498014176No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACAAAAGCTTCGGG3642581.1944389064065772No Hit
TACGATCTGCTGAGGGTAAGCCCTTC3531541.1580277648071102No Hit
CTGGCGGGCATGCCTGTTCGAGCGTCATT2822890.9256542463051086No Hit
TTCTGGCGGGCATGCCTGTTCGAGCGTCATT2604220.8539501366729451No Hit
TTTCTGGCGGGCATGCCTGTTCGAGCGTCATT2377790.7797014443785709No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCGC1808660.5930779481744587No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA1757270.5762266462400513No Hit
AATACCCTTCGGTGGGCTGAGG1643740.5389990083997461No Hit
AAATACCCTTCGGTGGGCTGAGG1622260.5319554986595034No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAG1510720.495380401991595No Hit
TCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTA1434140.4702690437091096No Hit
TTACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTTC1384160.45388009506770693No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAAG1234900.404936228036579No Hit
TTACGATCTGCTGAGGGTAAGCCCTTC1185770.3888260030115267No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGAC1165910.38231370769303413No Hit
AGCCCGTTGCACAATACCCTTCGGTGGGCTGAGG1120540.36743642478266114No Hit
TGAGGGTAAGCCCTTCTTCGCCTCGATTTC1071840.3514672011164684No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCGC894530.29332545474577787No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAG882220.2892888809607505No Hit
CGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA854060.2800549315061306No Hit
TAGCCCGTTGCACAATACCCTTCGGTGGGCTGAGGT839550.2752969554199611No Hit
GTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCC832450.2729687934480931No Hit
TACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCT779900.2557371157549016No Hit
AGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCC775980.254451708018321No Hit
GGCGGGCATGCCTGTTCGAGCGTCATT732250.2401121977324358No Hit
CAATACCCTTCGGTGGGCTGAGG713900.2340950467206363No Hit
ATGCCTGTTCGAGCGTCATT690000.22625799444913722No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCAT662660.21729293130676122No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACAAAAGCTTCGGGA649660.21303009952728474No Hit
GCGGGCATGCCTGTTCGAGCGTCATT638180.2092656911558701No Hit
AGGGCCCGAGTTGTAATTTG631270.2069998321100099No Hit
ACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTTC628350.2060423345103121No Hit
TCTTCTAAAGCTAAATACCG622590.20415357212186713No Hit
CCGGAGCTAGGGTTTAACGGCCGG611790.2006121426435328No Hit
CTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTA597120.195801700935462No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCA596430.1955754429410129No Hit
ATGGATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGTA584820.1917684062518035No Hit
TCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA579010.1898632483565144No Hit
AAATGGCTCAGTGAGGCTTTCGGACTGGCC578330.1896402694634341No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCCCGC571510.18740392232989334No Hit
AGGGCCCGAGTTGTAATT570790.18716782703133772No Hit
TCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAAC540200.17713705594409263No Hit
CCAGCGGAGGGATCATTA531360.17423833033404862No Hit
AGGGCCCGAGTTGTAATTT529090.17349397432332464No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATGGCC522830.17144125686643827No Hit
TCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA520320.1706182024228624No Hit
TACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTA519620.17038866532704447No Hit
AGGGCCCGAGTTGTAAT517920.16983121809434368No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAG517800.16979186887791775No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCC514520.16871632362894215No Hit
ACCAGCGGAGGGATCATTA496050.1626598234007167No Hit
CGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAAG494180.16204663144474585No Hit
ACGTCCCTTGAAAATCCGCGGGAA485640.15924627887576667No Hit
TTTCTTCTAAAGCTAAATACCG483960.15869538984580353No Hit
TCTTCTAAAGCTAAATACC481140.15777068325979401No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAAGG480160.15744933132564887No Hit
TACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTT461600.15136331918510396No Hit
AGGTGCCAGAGTAGACGCT461370.1512878998536209No Hit
ACGACCAGCGAATACCTGGTGTTGTATGT457420.14999265481293383No Hit
CCGGAGCTAGGGTTTAACGGCCG446030.14625775835383864No Hit
TTCGAGCGTCATT440440.14442474068866376No Hit
CTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAAC439490.1441132260586251No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGA437740.14353938331908017No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATGGC433000.14198508927025566No Hit
TCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAAG429680.14089642761580473No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCG429430.14081445008158405No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACAA424480.13919129490401416No Hit
ATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCG417950.1370500417101694No Hit
CGGGCATGCCTGTTCGAGCGTCATT411720.1350071615573895No Hit
GGCGCATCATCGACCGATCCTG405650.13301674702651087No Hit
ACGATCTGCTGAGGGTAAGCCCTTC398070.1305311881889392No Hit
TTGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCG386740.126815966338057No Hit
ATCCCGACTTCGGAAGGGATGTATTTATTAGATT384720.1261535878615537No Hit
TCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAA376600.12349095755006531No Hit
ATGGATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGTAG374440.12278267165439845No Hit
TCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAAG373580.12250066893667924No Hit
GGCCAGCATCAGCTCGCTGTCGGGGACA372590.12217603790116527No Hit
TTAGCCCGTTGCACAATACCCTTCGGTGGGCTGAGG371730.12189403518344603No Hit
AAGCGGAGGAAAAGAAACCAACAGGGATTGCCC368940.120979165901543No Hit
TCCGGAGCTAGGGTTTAA359930.11802469556822892No Hit
TCCCTTGAAAATCCGCGGGAA359200.11778532116830448No Hit
TCTGGCGGGCATGCCTGTTCGAGCGTCAT358190.11745413193005284No Hit
TTTCTTCTAAAGCTAAATACC357560.11724754854381668No Hit
TTGACCTCGGATCAGGTAGGAATACCCGCTGAACTT354940.11638842398518372No Hit
GGGCCCGAGTTGTAAT352000.11542436818274826No Hit
TTGACCTCGGATCAGGTAGGAATACCCGCTGAA351010.11509973714723426No Hit
ATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGT346770.11370939816685117No Hit
AAATACCCTTCGGTGGGCTGAGGT346430.11359790872031103No Hit
TTGGCGCTCAAGCGTCTCACCCATACCTCGC345610.11332902240806712No Hit
AGGCCAGCATCAGCTCGCTGTCGGGG343160.11252564257270424No Hit
CGCGAGGTTAAGGTGCCAGAGTAGACGCT339260.1112467930388613No Hit
ACAAAAGCTTCGGG331370.10865958205885594No Hit
AATACCCTTCGGTGGGCTGAGGT325610.10677081967041097No Hit
TGGCGGGCATGCCTGTTCGAGCGTCAT316980.10394095518911234No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGA314440.10310806344142999No Hit
GGGCCCGAGTTGTAATT311210.10204891369929854No Hit
ACGTCCCTTGAAAATCCGCGGGAAG310550.10183249300895589No Hit
TTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCC307860.10095041474074112No Hit

[OK]Adapter Content

Adapter graph