FastQCFastQC Report
Thu 21 Sept 2023
SRR18590154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18590154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30186713
Total Bases818 Mbp
Sequences flagged as poor quality0
Sequence length10-134
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGCGGGCATGCCTGTTCGAGCGTCATT6020671.9944768415163323No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCAT5875071.9462437000013881No Hit
TGGCGGGCATGCCTGTTCGAGCGTCATT4861551.610493331950385No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACA3922641.2994591362100274No Hit
TAGCCCGTTGCACAATACCCTTCGGTGGGCTGAGG2609940.8645989379499517No Hit
AAATACCCTTCGGTGGGCTGAGG2605480.8631214667194801No Hit
TACGATCTGCTGAGGGTAAGCCCTTC2302020.7625937941636772No Hit
CTGGCGGGCATGCCTGTTCGAGCGTCATT2298720.7615005979617588No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACAAAAGCTTCGGG2076880.688011311466737No Hit
TTCTGGCGGGCATGCCTGTTCGAGCGTCATT1926770.6382841351425046No Hit
AATACCCTTCGGTGGGCTGAGG1745710.5783041035305831No Hit
TTTCTGGCGGGCATGCCTGTTCGAGCGTCATT1619670.5365506340488281No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGAC1564600.5183075083398447No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCGC1466490.48580645398523514No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCAT1414840.4686962770673309No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCGC1254330.41552387634917387No Hit
TTACGATCTGCTGAGGGTAAGCCCTTC1167910.38689538672196605No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA1120040.3710374163626229No Hit
TCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTA1115290.3694638763750131No Hit
GTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCC1004230.3326728551068147No Hit
AGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCC998270.33069847651183487No Hit
AGCCCGTTGCACAATACCCTTCGGTGGGCTGAGG976920.3236258283569993No Hit
CCGGAGCTAGGGTTTAACGGCCGG826390.2737595179707045No Hit
GGCGGGCATGCCTGTTCGAGCGTCATT818440.2711259089388103No Hit
AGGTGCCAGAGTAGACGCT808750.26791588736408634No Hit
AAATGGCTCAGTGAGGCTTTCGGACTGGCC788420.26118113621711647No Hit
TGAGGGTAAGCCCTTCTTCGCCTCGATTTC729650.24171230567567922No Hit
TTGGCGCTCAAGCGTCTCACCCATACCTCGC728860.24145060113037148No Hit
ATGCCTGTTCGAGCGTCATT722460.23933046304180253No Hit
GGGGCCCGAGTTGTAAT720690.23874411235168266No Hit
TACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTTC717480.2376807305916348No Hit
AGGGCCCGAGTTGTAAT684810.22685808819264291No Hit
AGGGCCCGAGTTGTAATT676250.22402240349918193No Hit
AGGGCCCGAGTTGTAATTT665710.2205308010845699No Hit
CCGGAGCTAGGGTTTAACGGCCG663640.21984506892154834No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCA658980.2183013433758091No Hit
AGGGCCCGAGTTGTAATTTG656920.21761892392855095No Hit
CTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTA652810.21625739774979807No Hit
TTGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCG643650.21322295011053372No Hit
GCGGGCATGCCTGTTCGAGCGTCATT627450.20785635057384352No Hit
AAGCGGAGGAAAAGAAACCAACAGGGATTGCCC622170.20610723665077413No Hit
GGGCCCGAGTTGTAAT611720.2026454486780326No Hit
CGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA607350.2011977918894316No Hit
TGAATCATCGAATCTTTGAACGCACATTGCGCCCGC606390.20087977117614628No Hit
ACGACCAGCGAATACCTGGTGTTGTATGT582540.19297894408046348No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAAG569300.18859290840973642No Hit
TCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA539940.1788667749284263No Hit
CGCGAGGTTAAGGTGCCAGAGTAGACGCT537880.17818435548116815No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAG521030.17260242941985768No Hit
TCTGGCGGGCATGCCTGTTCGAGCGTCAT519210.17199951515092088No Hit
ATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCG511320.16938578241360694No Hit
GGCCAGCATCAGCTCGCTGTCGGGGACA505250.16737496394522983No Hit
TCCGGAGCTAGGGTTTA490450.16247214461541407No Hit
TGGCGCTCAAGCGTCTCACCCATACCTCGC489170.16204811699770028No Hit
CCGGAGCTAGGGTTTA480620.15921574502000266No Hit
ATGGATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGTA475900.15765214317968307No Hit
CAATACCCTTCGGTGGGCTGAGG465180.15410091188133004No Hit
AAGCGGCAACAGCTCAAATTTGAAATCTGGCC461900.15301434111093845No Hit
ACCAGCGGAGGGATCATTA461460.15286858161734931No Hit
TAGGTGGGAGCTTCGGCGCCGGTGAAATACCACTA459190.15211659513906003No Hit
GGGGCCCGAGTTGTAATT457090.15142092482874833No Hit
CCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGA442890.14671686844473592No Hit
AGGTGCCAGAGTAGACGC437160.14481868231231404No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCG433470.14359629019562348No Hit
TGGCGGGCATGCCTGTTCGAGCGTCAT433410.14357641390104317No Hit
AATGCTGCTCAAAATGGGAGGT431970.14309938283111515No Hit
GGGCCCGAGTTGTAATT429630.1423242073424821No Hit
ACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTTC428020.14179086010457648No Hit
TCTTCTAAAGCTAAATACC419800.13906780774707073No Hit
TTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCC419560.1389883025687494No Hit
ATGGCGCTCAAGCGTCTCACCCATACCTCGC413230.13689135349052411No Hit
ATCCCGACTTCGGAAGGGATGTATTTATTAGATT411340.1362652502112436No Hit
AAGGTGCCAGAGTAGACGCT409760.13574184112062815No Hit
TCTTCTAAAGCTAAATACCG407350.1349434766216514No Hit
ATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGT403490.13366476833698324No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGA401110.13287634198529663No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCG399780.1324357507887659No Hit
CCAGCGGAGGGATCATTA397010.13151812852230715No Hit
CGGGCATGCCTGTTCGAGCGTCATT390460.12934829969728734No Hit
TTGACCTCGGATCAGGTAGGAATACCCGCTGAACTT388550.12871557098648007No Hit
ACGATCTGCTGAGGGTAAGCCCTTC388420.128672505681556No Hit
TTGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGT388420.128672505681556No Hit
CGCGAGGTTAAGGTGCCAGAGTAGACGCTC387040.1282153509062083No Hit
ATGCTGCTCAAAATGGGAGGT386480.1280298388234585No Hit
GTGAAGCGGCAACAGCTCAAATTTGAAATCTGGC385410.12767537823677588No Hit
TCCGGAGCTAGGGTTTAA381820.12648611327771925No Hit
TTGGCGCTCAAGCGTCTCACCCATA373840.1238425660985348No Hit
AGCGGAGGAAAAGAAACCAACAGGGATTGCCC364180.12064248267110102No Hit
TTGGCGCTCAAGCGTCTCACCCAT357460.11841633767810361No Hit
CCGGAGCTAGGGTTTAA350090.11597486616048591No Hit
AGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGC343000.1136261506842431No Hit
ATGTTAGGGATAACAGG342880.11358639809508242No Hit
TGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCG341750.11321206121381946No Hit
CGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGA340180.11269196483896739No Hit
TTGGCGCTCAAGCGTCTCACCCATACCTCG339910.11260252151335588No Hit
TTGACCTCGGATCAGGTAGGAATACCCGCTGAA337080.11166502295231681No Hit
AGTGAGGCTTTCGGACTGGCC336880.11159876863704903No Hit
AGGTGCCAGAGTAGACGCTC330610.10952169585340411No Hit
TTACGATCTGCTGAGGGTAAGCCCTTCTTCGCCTCGATTTC330490.10948194326424343No Hit
AGGCCAGCATCAGCTCGCTGTCGGGGACAA329070.1090115376258422No Hit
AGGCCAGCATCAGCTCGCTGTCGGGG326190.10805747548598617No Hit
AAATACCCTTCGGTGGGCTGAGGT323990.10732867801804058No Hit
ATACCCTTCGGTGGGCTGAGG323360.10711997692494708No Hit
TCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCGGGAA322500.10683508336929563No Hit
TAGCCCGTTGCACAATACCCTTCGGTGGGCTGAGGT318810.10561269125260507No Hit
CTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAAC317880.10530460868660989No Hit
ATGGCGCTCAAGCGTCTCACCCATACCTCGCCCTCAGCGTA317150.10506278043588249No Hit
AGCGGCAACAGCTCAAATTTGAAATCTGGCC312510.10352568032167No Hit
TTAGCCCGTTGCACAATACCCTTCGGTGGGCTGAGG312180.10341636070147817No Hit
CTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAA309880.10265443607589868No Hit
GGGGCCCGAGTTGTAATTT304380.10083244240603473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)